Effects of warm acclimation on Na+,K+-ATPase α-subunit expression in chloride cells of antarctic fish

Philip R. Brauer, Jennifer N Sanmann, David H. Petzel

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The teleosts Trematomus bernacchii thrive in southern oceanic waters with temperatures below 0°C. These fish have serum osmolalities almost double those found in fish of temperate waters, thereby lowering their serum's freezing point and the energy needed for ionic homeostasis. Upon warm acclimation to 4°C, T. bernacchii decrease their serum osmolality and increase the Na +,K+-ATPase activity in their gills. Na+,K +-ATPase α-, α2-, and α3-subunit isoforms are expressed in the gills of T. bernacchii and it is thought that Na +,K+-ATPase subunit composition in chloride cells changes with warm acclimation. Using immunohistochemistry, we compared the number of chloride cells expressing various α-isoforms of the Na+,K +-ATPase in the gills of cold- and warm-acclimated T. bernacchii. We found no change in the number of α2- or α3-immunopositive cells in warm-acclimated fish gills or in the number of cells immunopositive for the Na+,K+,2Cl- cotransporter. However, the number of pan-α-immunopositive (recognizing all three α-isoforms) and α1-immunopositive cells both increased in warm-acclimated fish. This suggests that changes in the number of α1-isoform-expressing chloride cells could contribute to the increased Na+,K+-ATPase activity that occurs with warm-acclimation.

Original languageEnglish (US)
Pages (from-to)600-609
Number of pages10
JournalAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume285
Issue number1
DOIs
StatePublished - Jul 1 2005

Fingerprint

sodium-potassium-exchanging ATPase
Acclimatization
Chlorides
acclimation
chlorides
Fishes
Protein Isoforms
gills
fish
Osmolar Concentration
cells
osmolality
Cell Count
Serum
Water
Freezing
freezing point
Homeostasis
Immunohistochemistry
immunohistochemistry

Keywords

  • Acclimation
  • Chloride cell
  • Na,K-ATPase
  • Teleost

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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title = "Effects of warm acclimation on Na+,K+-ATPase α-subunit expression in chloride cells of antarctic fish",
abstract = "The teleosts Trematomus bernacchii thrive in southern oceanic waters with temperatures below 0°C. These fish have serum osmolalities almost double those found in fish of temperate waters, thereby lowering their serum's freezing point and the energy needed for ionic homeostasis. Upon warm acclimation to 4°C, T. bernacchii decrease their serum osmolality and increase the Na +,K+-ATPase activity in their gills. Na+,K +-ATPase α-, α2-, and α3-subunit isoforms are expressed in the gills of T. bernacchii and it is thought that Na +,K+-ATPase subunit composition in chloride cells changes with warm acclimation. Using immunohistochemistry, we compared the number of chloride cells expressing various α-isoforms of the Na+,K +-ATPase in the gills of cold- and warm-acclimated T. bernacchii. We found no change in the number of α2- or α3-immunopositive cells in warm-acclimated fish gills or in the number of cells immunopositive for the Na+,K+,2Cl- cotransporter. However, the number of pan-α-immunopositive (recognizing all three α-isoforms) and α1-immunopositive cells both increased in warm-acclimated fish. This suggests that changes in the number of α1-isoform-expressing chloride cells could contribute to the increased Na+,K+-ATPase activity that occurs with warm-acclimation.",
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N2 - The teleosts Trematomus bernacchii thrive in southern oceanic waters with temperatures below 0°C. These fish have serum osmolalities almost double those found in fish of temperate waters, thereby lowering their serum's freezing point and the energy needed for ionic homeostasis. Upon warm acclimation to 4°C, T. bernacchii decrease their serum osmolality and increase the Na +,K+-ATPase activity in their gills. Na+,K +-ATPase α-, α2-, and α3-subunit isoforms are expressed in the gills of T. bernacchii and it is thought that Na +,K+-ATPase subunit composition in chloride cells changes with warm acclimation. Using immunohistochemistry, we compared the number of chloride cells expressing various α-isoforms of the Na+,K +-ATPase in the gills of cold- and warm-acclimated T. bernacchii. We found no change in the number of α2- or α3-immunopositive cells in warm-acclimated fish gills or in the number of cells immunopositive for the Na+,K+,2Cl- cotransporter. However, the number of pan-α-immunopositive (recognizing all three α-isoforms) and α1-immunopositive cells both increased in warm-acclimated fish. This suggests that changes in the number of α1-isoform-expressing chloride cells could contribute to the increased Na+,K+-ATPase activity that occurs with warm-acclimation.

AB - The teleosts Trematomus bernacchii thrive in southern oceanic waters with temperatures below 0°C. These fish have serum osmolalities almost double those found in fish of temperate waters, thereby lowering their serum's freezing point and the energy needed for ionic homeostasis. Upon warm acclimation to 4°C, T. bernacchii decrease their serum osmolality and increase the Na +,K+-ATPase activity in their gills. Na+,K +-ATPase α-, α2-, and α3-subunit isoforms are expressed in the gills of T. bernacchii and it is thought that Na +,K+-ATPase subunit composition in chloride cells changes with warm acclimation. Using immunohistochemistry, we compared the number of chloride cells expressing various α-isoforms of the Na+,K +-ATPase in the gills of cold- and warm-acclimated T. bernacchii. We found no change in the number of α2- or α3-immunopositive cells in warm-acclimated fish gills or in the number of cells immunopositive for the Na+,K+,2Cl- cotransporter. However, the number of pan-α-immunopositive (recognizing all three α-isoforms) and α1-immunopositive cells both increased in warm-acclimated fish. This suggests that changes in the number of α1-isoform-expressing chloride cells could contribute to the increased Na+,K+-ATPase activity that occurs with warm-acclimation.

KW - Acclimation

KW - Chloride cell

KW - Na,K-ATPase

KW - Teleost

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