Effects of turnip crinkle virus infection on the structure and function of mitochondria and expression of stress proteins in turnips

James A. Blake, Kit W. Lee, Thomas J Morris, Thomas E Elthon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have investigated the effect of turnip crinkle virus (TCV) infection on mitochondrial structure and function in turnips (Brassica rapa cultivar Just Right). TCV infection resulted in plants with small, mottled leaves with severely crinkled edges, and in a 46% reduction in storage root mass. TCV infection resulted in specific vesicularization of mitochondrial outer membranes where TCV replication is thought to occur, with no apparent affect on other cellular membrane systems. Immunoblot analysis of mitochondrial proteins from storage roots indicated that the TCV p28 protein, which is essential for viral replication, was associated with mitochondria and that mitochondrial heat shock protein 70 and cpn60 levels increased upon TCV infection. Isolation of mitochondrial outer membranes further showed TCV p28 protein enrichment in the outer membrane as compared with total mitochondrial proteins or total cellular proteins. Analysis of mitochondrial electron transport chain activities indicated that TCV infection resulted in a 54% decrease in exogenous NADH-dependent oxygen uptake and a 8% decrease in succinate-dependent oxygen uptake. Together these results indicate that TCV infection induces a stress response in mitochondria and a reduction in the ability of mitochondria to supply adenosine 5′-triphosphate to the cell.

Original languageEnglish (US)
Pages (from-to)698-706
Number of pages9
JournalPhysiologia Plantarum
Volume129
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

Carmovirus
Turnip crinkle virus
Brassica napus
turnips
Virus Diseases
Heat-Shock Proteins
Mitochondria
mitochondria
infection
proteins
Mitochondrial Proteins
Mitochondrial Membranes
virus replication
Oxygen
uptake mechanisms
oxygen
Brassica rapa
Proteins
HSP70 Heat-Shock Proteins
Aptitude

ASJC Scopus subject areas

  • Plant Science

Cite this

Effects of turnip crinkle virus infection on the structure and function of mitochondria and expression of stress proteins in turnips. / Blake, James A.; Lee, Kit W.; Morris, Thomas J; Elthon, Thomas E.

In: Physiologia Plantarum, Vol. 129, No. 4, 01.04.2007, p. 698-706.

Research output: Contribution to journalArticle

Blake, James A. ; Lee, Kit W. ; Morris, Thomas J ; Elthon, Thomas E. / Effects of turnip crinkle virus infection on the structure and function of mitochondria and expression of stress proteins in turnips. In: Physiologia Plantarum. 2007 ; Vol. 129, No. 4. pp. 698-706.
@article{4a69289fcdea432e83f8cc96977214c7,
title = "Effects of turnip crinkle virus infection on the structure and function of mitochondria and expression of stress proteins in turnips",
abstract = "We have investigated the effect of turnip crinkle virus (TCV) infection on mitochondrial structure and function in turnips (Brassica rapa cultivar Just Right). TCV infection resulted in plants with small, mottled leaves with severely crinkled edges, and in a 46{\%} reduction in storage root mass. TCV infection resulted in specific vesicularization of mitochondrial outer membranes where TCV replication is thought to occur, with no apparent affect on other cellular membrane systems. Immunoblot analysis of mitochondrial proteins from storage roots indicated that the TCV p28 protein, which is essential for viral replication, was associated with mitochondria and that mitochondrial heat shock protein 70 and cpn60 levels increased upon TCV infection. Isolation of mitochondrial outer membranes further showed TCV p28 protein enrichment in the outer membrane as compared with total mitochondrial proteins or total cellular proteins. Analysis of mitochondrial electron transport chain activities indicated that TCV infection resulted in a 54{\%} decrease in exogenous NADH-dependent oxygen uptake and a 8{\%} decrease in succinate-dependent oxygen uptake. Together these results indicate that TCV infection induces a stress response in mitochondria and a reduction in the ability of mitochondria to supply adenosine 5′-triphosphate to the cell.",
author = "Blake, {James A.} and Lee, {Kit W.} and Morris, {Thomas J} and Elthon, {Thomas E}",
year = "2007",
month = "4",
day = "1",
doi = "10.1111/j.1399-3054.2006.00852.x",
language = "English (US)",
volume = "129",
pages = "698--706",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Effects of turnip crinkle virus infection on the structure and function of mitochondria and expression of stress proteins in turnips

AU - Blake, James A.

AU - Lee, Kit W.

AU - Morris, Thomas J

AU - Elthon, Thomas E

PY - 2007/4/1

Y1 - 2007/4/1

N2 - We have investigated the effect of turnip crinkle virus (TCV) infection on mitochondrial structure and function in turnips (Brassica rapa cultivar Just Right). TCV infection resulted in plants with small, mottled leaves with severely crinkled edges, and in a 46% reduction in storage root mass. TCV infection resulted in specific vesicularization of mitochondrial outer membranes where TCV replication is thought to occur, with no apparent affect on other cellular membrane systems. Immunoblot analysis of mitochondrial proteins from storage roots indicated that the TCV p28 protein, which is essential for viral replication, was associated with mitochondria and that mitochondrial heat shock protein 70 and cpn60 levels increased upon TCV infection. Isolation of mitochondrial outer membranes further showed TCV p28 protein enrichment in the outer membrane as compared with total mitochondrial proteins or total cellular proteins. Analysis of mitochondrial electron transport chain activities indicated that TCV infection resulted in a 54% decrease in exogenous NADH-dependent oxygen uptake and a 8% decrease in succinate-dependent oxygen uptake. Together these results indicate that TCV infection induces a stress response in mitochondria and a reduction in the ability of mitochondria to supply adenosine 5′-triphosphate to the cell.

AB - We have investigated the effect of turnip crinkle virus (TCV) infection on mitochondrial structure and function in turnips (Brassica rapa cultivar Just Right). TCV infection resulted in plants with small, mottled leaves with severely crinkled edges, and in a 46% reduction in storage root mass. TCV infection resulted in specific vesicularization of mitochondrial outer membranes where TCV replication is thought to occur, with no apparent affect on other cellular membrane systems. Immunoblot analysis of mitochondrial proteins from storage roots indicated that the TCV p28 protein, which is essential for viral replication, was associated with mitochondria and that mitochondrial heat shock protein 70 and cpn60 levels increased upon TCV infection. Isolation of mitochondrial outer membranes further showed TCV p28 protein enrichment in the outer membrane as compared with total mitochondrial proteins or total cellular proteins. Analysis of mitochondrial electron transport chain activities indicated that TCV infection resulted in a 54% decrease in exogenous NADH-dependent oxygen uptake and a 8% decrease in succinate-dependent oxygen uptake. Together these results indicate that TCV infection induces a stress response in mitochondria and a reduction in the ability of mitochondria to supply adenosine 5′-triphosphate to the cell.

UR - http://www.scopus.com/inward/record.url?scp=33947244353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947244353&partnerID=8YFLogxK

U2 - 10.1111/j.1399-3054.2006.00852.x

DO - 10.1111/j.1399-3054.2006.00852.x

M3 - Article

VL - 129

SP - 698

EP - 706

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 4

ER -