Effects of travoprost on aqueous humor dynamics in monkeys

Carol B Toris, Gui Lin Zhan, Carl B. Camras, Marsha A. McLaughlin

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Purpose: To determine the mechanism by which travoprost, a prodrug of a prostaglandin F analog, reduces intraocular pressure (IOP) in cynomolgus monkey eyes. Methods: One eye each of 12 monkeys was treated with laser burns to the trabecular meshwork to elevate IOP. At least 4 months later (Baseline Day), IOP was measured by pneumatonometry (9:00 AM and 11:45 AM), and aqueous flow and outflow facility were determined by a fluorophotometric method. Uveoscleral outflow was calculated. Both eyes were treated with travoprost 0.004% at 9:00 AM and 5:00 PM for two days and at 9:30 AM on the third day (Treatment Day), when measurements were repeated as on Baseline Day. Statistical analyses were performed using two-tailed, paired t tests. Results: On Treatment Day compared with Baseline Day, IOP in hypertensive eyes was reduced at 2.25 hours (25.8 ± 11.2 vs 33.7 ± 13.2 mm Hg; mean ± standard error of the mean [SEM]; P = 0.02) and 16 hours (26.3 ± 10.2 vs 35.1 ± 13.6 mm Hg; P = 0.02) after treatment. The increase in uveoscleral outflow was not significant. In normotensive eyes, IOP was reduced at 2.25 hours (19.0 ± 3.7 vs 23.0 ± 4.0 mm Hg; P = 0.03) and 16 hours (20.7 ± 5.4 vs 23.4 ± 5.3 mm Hg; P = 0.01) after treatment, and uveoscleral outflow was significantly (P = 0.02) increased (1.02 ± 0.43 vs 0.35 ± 0.72 μL/min). Conclusion: Travoprost reduces IOP in normotensive monkey eyes by increasing uveoscleral outflow. The IOP reduction in hypertensive eyes is probably via the same mechanism, although the increased uveoscleral drainage did not reach statistical significance. Travoprost had no effect on aqueous flow or outflow facility.

Original languageEnglish (US)
Pages (from-to)70-73
Number of pages4
JournalJournal of Glaucoma
Volume14
Issue number1
DOIs
StatePublished - Feb 1 2005

Fingerprint

Aqueous Humor
Intraocular Pressure
Haplorhini
Synthetic Prostaglandins
Trabecular Meshwork
Dinoprost
Macaca fascicularis
Prodrugs
Therapeutics
Travoprost
Burns
Drainage
Lasers

Keywords

  • Aqueous humor dynamics
  • Intraocular pressure
  • Monkey
  • Travoprost

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effects of travoprost on aqueous humor dynamics in monkeys. / Toris, Carol B; Zhan, Gui Lin; Camras, Carl B.; McLaughlin, Marsha A.

In: Journal of Glaucoma, Vol. 14, No. 1, 01.02.2005, p. 70-73.

Research output: Contribution to journalArticle

Toris, Carol B ; Zhan, Gui Lin ; Camras, Carl B. ; McLaughlin, Marsha A. / Effects of travoprost on aqueous humor dynamics in monkeys. In: Journal of Glaucoma. 2005 ; Vol. 14, No. 1. pp. 70-73.
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N2 - Purpose: To determine the mechanism by which travoprost, a prodrug of a prostaglandin F2α analog, reduces intraocular pressure (IOP) in cynomolgus monkey eyes. Methods: One eye each of 12 monkeys was treated with laser burns to the trabecular meshwork to elevate IOP. At least 4 months later (Baseline Day), IOP was measured by pneumatonometry (9:00 AM and 11:45 AM), and aqueous flow and outflow facility were determined by a fluorophotometric method. Uveoscleral outflow was calculated. Both eyes were treated with travoprost 0.004% at 9:00 AM and 5:00 PM for two days and at 9:30 AM on the third day (Treatment Day), when measurements were repeated as on Baseline Day. Statistical analyses were performed using two-tailed, paired t tests. Results: On Treatment Day compared with Baseline Day, IOP in hypertensive eyes was reduced at 2.25 hours (25.8 ± 11.2 vs 33.7 ± 13.2 mm Hg; mean ± standard error of the mean [SEM]; P = 0.02) and 16 hours (26.3 ± 10.2 vs 35.1 ± 13.6 mm Hg; P = 0.02) after treatment. The increase in uveoscleral outflow was not significant. In normotensive eyes, IOP was reduced at 2.25 hours (19.0 ± 3.7 vs 23.0 ± 4.0 mm Hg; P = 0.03) and 16 hours (20.7 ± 5.4 vs 23.4 ± 5.3 mm Hg; P = 0.01) after treatment, and uveoscleral outflow was significantly (P = 0.02) increased (1.02 ± 0.43 vs 0.35 ± 0.72 μL/min). Conclusion: Travoprost reduces IOP in normotensive monkey eyes by increasing uveoscleral outflow. The IOP reduction in hypertensive eyes is probably via the same mechanism, although the increased uveoscleral drainage did not reach statistical significance. Travoprost had no effect on aqueous flow or outflow facility.

AB - Purpose: To determine the mechanism by which travoprost, a prodrug of a prostaglandin F2α analog, reduces intraocular pressure (IOP) in cynomolgus monkey eyes. Methods: One eye each of 12 monkeys was treated with laser burns to the trabecular meshwork to elevate IOP. At least 4 months later (Baseline Day), IOP was measured by pneumatonometry (9:00 AM and 11:45 AM), and aqueous flow and outflow facility were determined by a fluorophotometric method. Uveoscleral outflow was calculated. Both eyes were treated with travoprost 0.004% at 9:00 AM and 5:00 PM for two days and at 9:30 AM on the third day (Treatment Day), when measurements were repeated as on Baseline Day. Statistical analyses were performed using two-tailed, paired t tests. Results: On Treatment Day compared with Baseline Day, IOP in hypertensive eyes was reduced at 2.25 hours (25.8 ± 11.2 vs 33.7 ± 13.2 mm Hg; mean ± standard error of the mean [SEM]; P = 0.02) and 16 hours (26.3 ± 10.2 vs 35.1 ± 13.6 mm Hg; P = 0.02) after treatment. The increase in uveoscleral outflow was not significant. In normotensive eyes, IOP was reduced at 2.25 hours (19.0 ± 3.7 vs 23.0 ± 4.0 mm Hg; P = 0.03) and 16 hours (20.7 ± 5.4 vs 23.4 ± 5.3 mm Hg; P = 0.01) after treatment, and uveoscleral outflow was significantly (P = 0.02) increased (1.02 ± 0.43 vs 0.35 ± 0.72 μL/min). Conclusion: Travoprost reduces IOP in normotensive monkey eyes by increasing uveoscleral outflow. The IOP reduction in hypertensive eyes is probably via the same mechanism, although the increased uveoscleral drainage did not reach statistical significance. Travoprost had no effect on aqueous flow or outflow facility.

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