Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection

Liqian Zhu, Jesse Thompson, Fangrui Ma, James D Eudy, Clinton Jones

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Sensory neurons are a primary site for life-long latency of bovine herpesvirus 1 (BoHV-1). The synthetic corticosteroid dexamethasone induces reactivation from latency and productive infection, in part because the BoHV-1 genome contains more than 100 glucocorticoid receptor (GR) responsive elements (GREs). Two GREs in the immediate early transcription unit 1 promoter are required for dexamethasone induction. Recent studies also demonstrated that the serum and glucocorticoid receptor protein kinase (SGK) family stimulated BoHV-1 replication. Consequently, we hypothesized that dexamethasone influences several aspects of productive infection. In this study, we demonstrated that dexamethasone increased expression of the immediate early protein bICP4, certain late transcripts, and UL23 (thymidine kinase) by four hours after infection. SGK1 expression and Akt phosphorylation were also stimulated during early stages of infection and dexamethasone treatment further increased this effect. These studies suggest that stress, as mimicked by dexamethasone treatment, has the potential to stimulate productive infection by multiple pathways.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalVirology
Volume505
DOIs
StatePublished - May 1 2017

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Bovine Herpesvirus 1
Dexamethasone
Adrenal Cortex Hormones
Infection
Glucocorticoid Receptors
Immediate-Early Proteins
Thymidine Kinase
Sensory Receptor Cells
Protein Kinases
Phosphorylation
Genome
Serum

ASJC Scopus subject areas

  • Virology

Cite this

Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection. / Zhu, Liqian; Thompson, Jesse; Ma, Fangrui; Eudy, James D; Jones, Clinton.

In: Virology, Vol. 505, 01.05.2017, p. 71-79.

Research output: Contribution to journalArticle

Zhu, Liqian ; Thompson, Jesse ; Ma, Fangrui ; Eudy, James D ; Jones, Clinton. / Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection. In: Virology. 2017 ; Vol. 505. pp. 71-79.
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