Effects of the antimicrobial tylosin on the microbial community structure of an anaerobic sequencing batch reactor

Toshio Shimada, Xu Li, Julie L. Zilles, Eberhard Morgenroth, Lutgarde Raskin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of the antimicrobial tylosin on a methanogenic microbial community were studied in a glucose-fed laboratory-scale anaerobic sequencing batch reactor (ASBR) exposed to stepwise increases of tylosin (0, 1.67, and 167 mg/L). The microbial community structure was determined using quantitative fluorescence in situ hybridization (FISH) and phylogenetic analyses of bacterial 16S ribosomal RNA (rRNA) gene clone libraries of biomass samples. During the periods without tylosin addition and with an influent tylosin concentration of 1.67 mg/L, 16S rRNA gene sequences related to Syntrophobacter were detected and the relative abundance of Methanosaeta species was high. During the highest tylosin dose of 167 mg/L, 16S rRNA gene sequences related to Syntrophobacter species were not detected and the relative abundance of Methanosaeta decreased considerably. Throughout the experimental period, Propionibacteriaceae and high GC Gram-positive bacteria were present, based on 16S rRNA gene sequences and FISH analyses, respectively. The accumulation of propionate and subsequent reactor failure after long-term exposure to tylosin are attributed to the direct inhibition of propionate-oxidizing syntrophic bacteria closely related to Syntrophobacter and the indirect inhibition of Methanosaeta by high propionate concentrations and low pH. Biotechnol.

Original languageEnglish (US)
Pages (from-to)296-305
Number of pages10
JournalBiotechnology and Bioengineering
Volume108
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Tylosin
Batch reactors
Genes
16S Ribosomal RNA
Propionates
rRNA Genes
Bacteria
Fluorescence
Propionibacteriaceae
Fluorescence In Situ Hybridization
Glucose
Biomass
Gram-Positive Bacteria
Gene Library
Clone Cells
Ribosomal RNA

Keywords

  • Anaerobic
  • Antimicrobial
  • Macrolide
  • Methanogenesis
  • Propionate
  • Tylosin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Effects of the antimicrobial tylosin on the microbial community structure of an anaerobic sequencing batch reactor. / Shimada, Toshio; Li, Xu; Zilles, Julie L.; Morgenroth, Eberhard; Raskin, Lutgarde.

In: Biotechnology and Bioengineering, Vol. 108, No. 2, 01.02.2011, p. 296-305.

Research output: Contribution to journalArticle

Shimada, Toshio ; Li, Xu ; Zilles, Julie L. ; Morgenroth, Eberhard ; Raskin, Lutgarde. / Effects of the antimicrobial tylosin on the microbial community structure of an anaerobic sequencing batch reactor. In: Biotechnology and Bioengineering. 2011 ; Vol. 108, No. 2. pp. 296-305.
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