Effects of temperature and flow rate on frontal and elution chromatography of aggregating systems

Kevin E. Van Cott, Roger D. Whitley, N. H.Linda Wang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

During chromatography, β-lactoglobulin A can aggregate into tetramers, octamers, and dodecamers. Increasing temperature changes relative peak heights and results in peak merging. In the literature, temperature-induced shifts in equilibrium distributions were hypothesized to cause these changes. Using a reaction-separation model, we have shown that an increase in reaction rates in accordance with the Arrhenius relationship also explains the literature data well. When aggregation rates are fast relative to mass transfer rates, there is a loss of resolution between individual aggregates; breakthrough times and retention times of concentration wave fronts and peaks are averaged, resulting in a single front or peak. Conversely, when reaction rates are relatively slow, wave fronts and peaks of individual aggregates are distinct. Scaling up a complex reacting system is difficult because changes in apparently unrelated parameters, such as particle size, flow rate, concentration, and temperature, can all alter effective reaction rates. This study shows that a dimensionless group approach provides a simple method to predict surprising results. Most surprising is that at low temperatures (slow reaction rates) a decrease in flow rate results in a loss of resolution between the aggregates, whereas at high temperatures (fast reaction rates) an increase in flow rate can enhance resolution.

Original languageEnglish (US)
Pages (from-to)142-152
Number of pages11
JournalSeparations Technology
Volume1
Issue number3
DOIs
StatePublished - 1991

Fingerprint

Chromatography
Reaction rates
Flow rate
Temperature
Lactoglobulins
Merging
Large scale systems
Mass transfer
Agglomeration
Particle size

Keywords

  • aggregation
  • chromatography
  • reaction
  • β-lactoglobulin A

ASJC Scopus subject areas

  • Engineering(all)
  • Filtration and Separation

Cite this

Effects of temperature and flow rate on frontal and elution chromatography of aggregating systems. / Van Cott, Kevin E.; Whitley, Roger D.; Wang, N. H.Linda.

In: Separations Technology, Vol. 1, No. 3, 1991, p. 142-152.

Research output: Contribution to journalArticle

@article{f1e5b718f9dd43edb301d8dd70e357e7,
title = "Effects of temperature and flow rate on frontal and elution chromatography of aggregating systems",
abstract = "During chromatography, β-lactoglobulin A can aggregate into tetramers, octamers, and dodecamers. Increasing temperature changes relative peak heights and results in peak merging. In the literature, temperature-induced shifts in equilibrium distributions were hypothesized to cause these changes. Using a reaction-separation model, we have shown that an increase in reaction rates in accordance with the Arrhenius relationship also explains the literature data well. When aggregation rates are fast relative to mass transfer rates, there is a loss of resolution between individual aggregates; breakthrough times and retention times of concentration wave fronts and peaks are averaged, resulting in a single front or peak. Conversely, when reaction rates are relatively slow, wave fronts and peaks of individual aggregates are distinct. Scaling up a complex reacting system is difficult because changes in apparently unrelated parameters, such as particle size, flow rate, concentration, and temperature, can all alter effective reaction rates. This study shows that a dimensionless group approach provides a simple method to predict surprising results. Most surprising is that at low temperatures (slow reaction rates) a decrease in flow rate results in a loss of resolution between the aggregates, whereas at high temperatures (fast reaction rates) an increase in flow rate can enhance resolution.",
keywords = "aggregation, chromatography, reaction, β-lactoglobulin A",
author = "{Van Cott}, {Kevin E.} and Whitley, {Roger D.} and Wang, {N. H.Linda}",
year = "1991",
doi = "10.1016/0956-9618(91)80009-O",
language = "English (US)",
volume = "1",
pages = "142--152",
journal = "Separation and Purification Technology",
issn = "1383-5866",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Effects of temperature and flow rate on frontal and elution chromatography of aggregating systems

AU - Van Cott, Kevin E.

AU - Whitley, Roger D.

AU - Wang, N. H.Linda

PY - 1991

Y1 - 1991

N2 - During chromatography, β-lactoglobulin A can aggregate into tetramers, octamers, and dodecamers. Increasing temperature changes relative peak heights and results in peak merging. In the literature, temperature-induced shifts in equilibrium distributions were hypothesized to cause these changes. Using a reaction-separation model, we have shown that an increase in reaction rates in accordance with the Arrhenius relationship also explains the literature data well. When aggregation rates are fast relative to mass transfer rates, there is a loss of resolution between individual aggregates; breakthrough times and retention times of concentration wave fronts and peaks are averaged, resulting in a single front or peak. Conversely, when reaction rates are relatively slow, wave fronts and peaks of individual aggregates are distinct. Scaling up a complex reacting system is difficult because changes in apparently unrelated parameters, such as particle size, flow rate, concentration, and temperature, can all alter effective reaction rates. This study shows that a dimensionless group approach provides a simple method to predict surprising results. Most surprising is that at low temperatures (slow reaction rates) a decrease in flow rate results in a loss of resolution between the aggregates, whereas at high temperatures (fast reaction rates) an increase in flow rate can enhance resolution.

AB - During chromatography, β-lactoglobulin A can aggregate into tetramers, octamers, and dodecamers. Increasing temperature changes relative peak heights and results in peak merging. In the literature, temperature-induced shifts in equilibrium distributions were hypothesized to cause these changes. Using a reaction-separation model, we have shown that an increase in reaction rates in accordance with the Arrhenius relationship also explains the literature data well. When aggregation rates are fast relative to mass transfer rates, there is a loss of resolution between individual aggregates; breakthrough times and retention times of concentration wave fronts and peaks are averaged, resulting in a single front or peak. Conversely, when reaction rates are relatively slow, wave fronts and peaks of individual aggregates are distinct. Scaling up a complex reacting system is difficult because changes in apparently unrelated parameters, such as particle size, flow rate, concentration, and temperature, can all alter effective reaction rates. This study shows that a dimensionless group approach provides a simple method to predict surprising results. Most surprising is that at low temperatures (slow reaction rates) a decrease in flow rate results in a loss of resolution between the aggregates, whereas at high temperatures (fast reaction rates) an increase in flow rate can enhance resolution.

KW - aggregation

KW - chromatography

KW - reaction

KW - β-lactoglobulin A

UR - http://www.scopus.com/inward/record.url?scp=0025846880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025846880&partnerID=8YFLogxK

U2 - 10.1016/0956-9618(91)80009-O

DO - 10.1016/0956-9618(91)80009-O

M3 - Article

AN - SCOPUS:0025846880

VL - 1

SP - 142

EP - 152

JO - Separation and Purification Technology

JF - Separation and Purification Technology

SN - 1383-5866

IS - 3

ER -