Effects of surface-modified scaffolds on the growth and differentiation of mouse adipose-derived stromal cells

Jing Lin, Merry L. Lindsey, Beili Zhu, C. Mauli Agrawal, Steven R. Bailey

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Purpose Adipose-derived stromal cells (ADSCs) have been shown to increase angiogenesis in ischemic tissue. Maintaining cell survival and facilitating angiogenesis in ischemic tissue, however, continues to be the major challenge of ADSCs implantation. Recently, bioengineered scaffolds were introduced to support and facilitate cell culture and differentiation. The effects of a surface modified three-dimensional (3D) scaffold on ADSC function have not been investigated. Accordingly, the objective of this study was to determine the influence of a gas-plasma treated scaffold on ADSC growth, differentiation into endothelial cell, and angiogenic gene expression. Methods Freshly isolated mouse ADSCs were characterized by flow cytometry and cultured into wells containing gas-plasma treated scaffolds, non-treated scaffolds, or control wells. Either endothelial growth media or differentiation media was used to alter cell environment. After 3 and 6 days, cell proliferation was analyzed. VEGF concentration in the medium was measured by ELISA. Gene expression was quantified by real-time PCR for VEGF receptor-2 (KDR), cyclooxygenase-2 (COX-2) and matrix metalloproteinases-2 (MMP-2). Results ADSCs expressed stem/endothelial progenitor markers CD34 and CD133 and endothelial cell marker CD31. ADSCs grew in the 3D scaffold. Cells grown on gas-plasma treated scaffolds displayed significantly increased expression of VEGF, COX-2, and MMP-2 when grown in differentiation but not growth media. When cultured in endothelial growth media, VEGF secretion and the expression of KDR, COX-2 and MMP-2 were lower in 3D scaffolds than controls.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume1
Issue number3
DOIs
StatePublished - May 1 2007

Fingerprint

Stromal Cells
Scaffolds
Plasma Gases
Matrix Metalloproteinase 2
Cyclooxygenase 2
Growth
Vascular Endothelial Growth Factor A
Endothelial cells
Scaffolds (biology)
Plasmas
Gene expression
Gases
Cells
Cell Differentiation
Tissue
Endothelial Cells
Vascular Endothelial Growth Factor Receptor
Flow cytometry
Oil and Gas Fields
Gene Expression

Keywords

  • Adipose-derived stromal cell
  • Angiogenesis
  • Differentiation
  • Gas plasma
  • Heart failure
  • Surface-modified scaffolds

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Effects of surface-modified scaffolds on the growth and differentiation of mouse adipose-derived stromal cells. / Lin, Jing; Lindsey, Merry L.; Zhu, Beili; Mauli Agrawal, C.; Bailey, Steven R.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 1, No. 3, 01.05.2007, p. 211-217.

Research output: Contribution to journalArticle

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N2 - Purpose Adipose-derived stromal cells (ADSCs) have been shown to increase angiogenesis in ischemic tissue. Maintaining cell survival and facilitating angiogenesis in ischemic tissue, however, continues to be the major challenge of ADSCs implantation. Recently, bioengineered scaffolds were introduced to support and facilitate cell culture and differentiation. The effects of a surface modified three-dimensional (3D) scaffold on ADSC function have not been investigated. Accordingly, the objective of this study was to determine the influence of a gas-plasma treated scaffold on ADSC growth, differentiation into endothelial cell, and angiogenic gene expression. Methods Freshly isolated mouse ADSCs were characterized by flow cytometry and cultured into wells containing gas-plasma treated scaffolds, non-treated scaffolds, or control wells. Either endothelial growth media or differentiation media was used to alter cell environment. After 3 and 6 days, cell proliferation was analyzed. VEGF concentration in the medium was measured by ELISA. Gene expression was quantified by real-time PCR for VEGF receptor-2 (KDR), cyclooxygenase-2 (COX-2) and matrix metalloproteinases-2 (MMP-2). Results ADSCs expressed stem/endothelial progenitor markers CD34 and CD133 and endothelial cell marker CD31. ADSCs grew in the 3D scaffold. Cells grown on gas-plasma treated scaffolds displayed significantly increased expression of VEGF, COX-2, and MMP-2 when grown in differentiation but not growth media. When cultured in endothelial growth media, VEGF secretion and the expression of KDR, COX-2 and MMP-2 were lower in 3D scaffolds than controls.

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