Effects of substrate stiffness on adipogenic and osteogenic differentiation of human mesenchymal stem cells

Wen Zhao, Xiaowei Li, Xiaoyan Liu, Ning Zhang, Xuejun Wen

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Substrate mechanical properties, in addition to biochemical signals, have been shown to modulate cell phenotype. In this study, we inspected the effects of substrate stiffness on human mesenchymal stem cells (hMSCs) derived from adult human bone marrow differentiation into adipogenic and osteogenic cells. A chemically modified extracellular matrix derived and highly biocompatible hydrogel, based on thiol functionalized hyaluronic acid (HA-SH) and thiol functionalized recombinant human gelatin (Gtn-SH), which can be crosslinked by poly (ethylene glycol) tetra-acrylate (PEGTA), was used as a model system. The stiffness of the hydrogel was controlled by adjusting the crosslinking density. Human bone marrow MSCs were cultured on the hydrogels with different stiffness under adipogenic and osteogenic conditions. Oil Red O staining and F-actin staining were applied to assess the change of cell morphologies under adipogenic and osteogenic differentiation, respectively. Gene expression of cells was determined with reverse transcription polymerase chain reaction (RT-PCR) as a function of hydrogel stiffness. Results support the hypothesis that adipogenic and osteogenic differentiation of hMSCs are inclined to occur on substrate with stiffness similar to their in vivo microenvironments.

Original languageEnglish (US)
Pages (from-to)316-323
Number of pages8
JournalMaterials Science and Engineering C
Volume40
DOIs
StatePublished - Jul 1 2014

Fingerprint

stem cells
Stem cells
stiffness
Hydrogels
Stiffness
Hydrogel
Substrates
bone marrow
staining
Sulfhydryl Compounds
thiols
Bone
cells
Hyaluronic acid
polymerase chain reaction
phenotype
gene expression
Polymerase chain reaction
gelatins
Hyaluronic Acid

Keywords

  • Differentiation
  • Hydrogel
  • Mechanical property
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Effects of substrate stiffness on adipogenic and osteogenic differentiation of human mesenchymal stem cells. / Zhao, Wen; Li, Xiaowei; Liu, Xiaoyan; Zhang, Ning; Wen, Xuejun.

In: Materials Science and Engineering C, Vol. 40, 01.07.2014, p. 316-323.

Research output: Contribution to journalArticle

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