Effects of spray-dried porcine plasma on fecal microbiota in nursery pigs

Huyen Tran, Christopher L. Anderson, Justin W. Bundy, Samodha C Fernando, Phillip S Miller, Thomas E Burkey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Spray-dried porcine plasma (SDPP) has been considered as an alternative for in-feed antibiotics to improve pig growth performance; however, the effect of SDPP on gut microbiota is unknown. The objective of this study was to evaluate effects of feeding SDPP on fecal microbial communities of nursery pigs. Ninety-six weaned pigs were assigned to 16 pens, which were allotted to two dietary treatments, including the control or the control + SDPP (5% and 2.5% SDPP inclusion in phase 1 and 2, re-spectively) diet. Fecal samples were collected at d 0, 7, 14, 21, and 28. Multiplex sequencing of V3 region of the 16S rRNA gene was used to characterize the bacterial community structure of fecal samples. Pearson’s correlation tests were performed in Calypso to identify bacterial taxa that were either positively or negatively associated with overall growth performance. Feeding SDPP altered microbial structure at family, genus, and operational taxonomic unit (OTU) classifications; however, fecal microbes shifted with time. At the family level, Clostridiaceae increased (P < 0.001) on d 14, but decreased (P < 0.05) on d 28 in SDPP-fed pigs compared with control pigs. Decreased Veillonellaceae (P < 0.05; d 14) and Lachnospiraceae (P = 0.001; overall) were observed in SDPP-fed pigs compared with control pigs. Feeding SDPP increased lactic acid–producing bacteria (Lactobacillus delbrueckii, d 7) and cellulolytic bacteria (Ruminococcus albus, d 7; Clostridium thermocel-lum, d 7 and 14; and Clostridium saccharoperbuty-lacetonicum/beijerinckii, d 14; and Megasphaera elsdenii, d 21). On d 28, feeding SDPP decreased (P < 0.05) Clostridium difficile compared with control pigs. In conclusion, feeding SDPP altered fecal microbial communities in nursery pigs. The results of this study may provide information to help explain the positive effects associated with feeding SDPP on nutrient digestibility and gut health of nursery pigs.

Original languageEnglish (US)
Pages (from-to)1017-1031
Number of pages15
JournalJournal of animal science
Volume96
Issue number3
DOIs
StatePublished - Apr 3 2018

Fingerprint

Nurseries
Microbiota
Swine
swine
microbiome
Clostridium
Veillonellaceae
microbial communities
growth performance
Clostridiaceae
Megasphaera elsdenii
Ruminococcus
Calypso
Ruminococcus albus
Lactobacillus delbrueckii
Bacteria
Bacterial Structures
Clostridium difficile

Keywords

  • Fecal microbiota
  • Pigs
  • Spray-dried porcine plasma

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Effects of spray-dried porcine plasma on fecal microbiota in nursery pigs. / Tran, Huyen; Anderson, Christopher L.; Bundy, Justin W.; Fernando, Samodha C; Miller, Phillip S; Burkey, Thomas E.

In: Journal of animal science, Vol. 96, No. 3, 03.04.2018, p. 1017-1031.

Research output: Contribution to journalArticle

Tran, Huyen ; Anderson, Christopher L. ; Bundy, Justin W. ; Fernando, Samodha C ; Miller, Phillip S ; Burkey, Thomas E. / Effects of spray-dried porcine plasma on fecal microbiota in nursery pigs. In: Journal of animal science. 2018 ; Vol. 96, No. 3. pp. 1017-1031.
@article{28af0c5ae712482cbc753e31394c6505,
title = "Effects of spray-dried porcine plasma on fecal microbiota in nursery pigs",
abstract = "Spray-dried porcine plasma (SDPP) has been considered as an alternative for in-feed antibiotics to improve pig growth performance; however, the effect of SDPP on gut microbiota is unknown. The objective of this study was to evaluate effects of feeding SDPP on fecal microbial communities of nursery pigs. Ninety-six weaned pigs were assigned to 16 pens, which were allotted to two dietary treatments, including the control or the control + SDPP (5{\%} and 2.5{\%} SDPP inclusion in phase 1 and 2, re-spectively) diet. Fecal samples were collected at d 0, 7, 14, 21, and 28. Multiplex sequencing of V3 region of the 16S rRNA gene was used to characterize the bacterial community structure of fecal samples. Pearson’s correlation tests were performed in Calypso to identify bacterial taxa that were either positively or negatively associated with overall growth performance. Feeding SDPP altered microbial structure at family, genus, and operational taxonomic unit (OTU) classifications; however, fecal microbes shifted with time. At the family level, Clostridiaceae increased (P < 0.001) on d 14, but decreased (P < 0.05) on d 28 in SDPP-fed pigs compared with control pigs. Decreased Veillonellaceae (P < 0.05; d 14) and Lachnospiraceae (P = 0.001; overall) were observed in SDPP-fed pigs compared with control pigs. Feeding SDPP increased lactic acid–producing bacteria (Lactobacillus delbrueckii, d 7) and cellulolytic bacteria (Ruminococcus albus, d 7; Clostridium thermocel-lum, d 7 and 14; and Clostridium saccharoperbuty-lacetonicum/beijerinckii, d 14; and Megasphaera elsdenii, d 21). On d 28, feeding SDPP decreased (P < 0.05) Clostridium difficile compared with control pigs. In conclusion, feeding SDPP altered fecal microbial communities in nursery pigs. The results of this study may provide information to help explain the positive effects associated with feeding SDPP on nutrient digestibility and gut health of nursery pigs.",
keywords = "Fecal microbiota, Pigs, Spray-dried porcine plasma",
author = "Huyen Tran and Anderson, {Christopher L.} and Bundy, {Justin W.} and Fernando, {Samodha C} and Miller, {Phillip S} and Burkey, {Thomas E}",
year = "2018",
month = "4",
day = "3",
doi = "10.1093/jas/skx034",
language = "English (US)",
volume = "96",
pages = "1017--1031",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "3",

}

TY - JOUR

T1 - Effects of spray-dried porcine plasma on fecal microbiota in nursery pigs

AU - Tran, Huyen

AU - Anderson, Christopher L.

AU - Bundy, Justin W.

AU - Fernando, Samodha C

AU - Miller, Phillip S

AU - Burkey, Thomas E

PY - 2018/4/3

Y1 - 2018/4/3

N2 - Spray-dried porcine plasma (SDPP) has been considered as an alternative for in-feed antibiotics to improve pig growth performance; however, the effect of SDPP on gut microbiota is unknown. The objective of this study was to evaluate effects of feeding SDPP on fecal microbial communities of nursery pigs. Ninety-six weaned pigs were assigned to 16 pens, which were allotted to two dietary treatments, including the control or the control + SDPP (5% and 2.5% SDPP inclusion in phase 1 and 2, re-spectively) diet. Fecal samples were collected at d 0, 7, 14, 21, and 28. Multiplex sequencing of V3 region of the 16S rRNA gene was used to characterize the bacterial community structure of fecal samples. Pearson’s correlation tests were performed in Calypso to identify bacterial taxa that were either positively or negatively associated with overall growth performance. Feeding SDPP altered microbial structure at family, genus, and operational taxonomic unit (OTU) classifications; however, fecal microbes shifted with time. At the family level, Clostridiaceae increased (P < 0.001) on d 14, but decreased (P < 0.05) on d 28 in SDPP-fed pigs compared with control pigs. Decreased Veillonellaceae (P < 0.05; d 14) and Lachnospiraceae (P = 0.001; overall) were observed in SDPP-fed pigs compared with control pigs. Feeding SDPP increased lactic acid–producing bacteria (Lactobacillus delbrueckii, d 7) and cellulolytic bacteria (Ruminococcus albus, d 7; Clostridium thermocel-lum, d 7 and 14; and Clostridium saccharoperbuty-lacetonicum/beijerinckii, d 14; and Megasphaera elsdenii, d 21). On d 28, feeding SDPP decreased (P < 0.05) Clostridium difficile compared with control pigs. In conclusion, feeding SDPP altered fecal microbial communities in nursery pigs. The results of this study may provide information to help explain the positive effects associated with feeding SDPP on nutrient digestibility and gut health of nursery pigs.

AB - Spray-dried porcine plasma (SDPP) has been considered as an alternative for in-feed antibiotics to improve pig growth performance; however, the effect of SDPP on gut microbiota is unknown. The objective of this study was to evaluate effects of feeding SDPP on fecal microbial communities of nursery pigs. Ninety-six weaned pigs were assigned to 16 pens, which were allotted to two dietary treatments, including the control or the control + SDPP (5% and 2.5% SDPP inclusion in phase 1 and 2, re-spectively) diet. Fecal samples were collected at d 0, 7, 14, 21, and 28. Multiplex sequencing of V3 region of the 16S rRNA gene was used to characterize the bacterial community structure of fecal samples. Pearson’s correlation tests were performed in Calypso to identify bacterial taxa that were either positively or negatively associated with overall growth performance. Feeding SDPP altered microbial structure at family, genus, and operational taxonomic unit (OTU) classifications; however, fecal microbes shifted with time. At the family level, Clostridiaceae increased (P < 0.001) on d 14, but decreased (P < 0.05) on d 28 in SDPP-fed pigs compared with control pigs. Decreased Veillonellaceae (P < 0.05; d 14) and Lachnospiraceae (P = 0.001; overall) were observed in SDPP-fed pigs compared with control pigs. Feeding SDPP increased lactic acid–producing bacteria (Lactobacillus delbrueckii, d 7) and cellulolytic bacteria (Ruminococcus albus, d 7; Clostridium thermocel-lum, d 7 and 14; and Clostridium saccharoperbuty-lacetonicum/beijerinckii, d 14; and Megasphaera elsdenii, d 21). On d 28, feeding SDPP decreased (P < 0.05) Clostridium difficile compared with control pigs. In conclusion, feeding SDPP altered fecal microbial communities in nursery pigs. The results of this study may provide information to help explain the positive effects associated with feeding SDPP on nutrient digestibility and gut health of nursery pigs.

KW - Fecal microbiota

KW - Pigs

KW - Spray-dried porcine plasma

UR - http://www.scopus.com/inward/record.url?scp=85045135870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045135870&partnerID=8YFLogxK

U2 - 10.1093/jas/skx034

DO - 10.1093/jas/skx034

M3 - Article

C2 - 29385463

AN - SCOPUS:85045135870

VL - 96

SP - 1017

EP - 1031

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 3

ER -