Effects of smokeless tobacco and nicotine on cytokine responses of murine memory T cells

Thomas M Petro, Shaobin Zhang

Research output: Contribution to journalArticle

Abstract

Smokeless tobacco (ST) is associated with oral cancer and periodontal disease. These effects are partially due to ST and nicotine's influence on naive T cell cytokine responses. It is unclear if the effect of ST or nicotine could be seen during secondary stimulation of memory T cells. To determine the influence of ST on memory T cells, splenic mononuclear cells (SPM) were exposed to 1:102 to 1:103 dilutions of ST, 10-100 ug/ml nicotine, or medium during 4 days of stimulation with anti-CD3. SPM were then washed extensively and restimulated with anti-CD3 plus anti-CD28 without ST or nicotine. Production and expression of IL-2, IL-4, IL-10 and IFN-g were evaluated using ELISA and RT-PCR. T cells exposed to 10 ug/ml nicotine during primary stimulation produced 2-fold more IFN-g during secondary responses compared with controls. Sustained IL-10 gene expression was noted even 4 days after primary stimulation in the presence of nicotine, which resulted in 30% more IL-10 accumulation during secondary stimulation compared with controls. Exposure to ST during the primary T cell response led to significantly higher production of IL-2 (+270%), IL-4 (+69%), IFN-g (+300%), and IL-10 (+73%) during secondary responses without ST. These results indicate that exposure to ST or nicotine during the primary immune response will significantly influence cytokine production during the response of memory T cells.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

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smokeless tobacco
Smokeless Tobacco
T-cells
nicotine
Nicotine
cytokines
T-lymphocytes
Cytokines
T-Lymphocytes
Data storage equipment
mice
interleukin-10
Interleukin-10
interleukin-4
interleukin-2
Interleukin-4
Interleukin-2
Mouth Diseases
Mouth Neoplasms
Periodontal Diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Effects of smokeless tobacco and nicotine on cytokine responses of murine memory T cells. / Petro, Thomas M; Zhang, Shaobin.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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abstract = "Smokeless tobacco (ST) is associated with oral cancer and periodontal disease. These effects are partially due to ST and nicotine's influence on naive T cell cytokine responses. It is unclear if the effect of ST or nicotine could be seen during secondary stimulation of memory T cells. To determine the influence of ST on memory T cells, splenic mononuclear cells (SPM) were exposed to 1:102 to 1:103 dilutions of ST, 10-100 ug/ml nicotine, or medium during 4 days of stimulation with anti-CD3. SPM were then washed extensively and restimulated with anti-CD3 plus anti-CD28 without ST or nicotine. Production and expression of IL-2, IL-4, IL-10 and IFN-g were evaluated using ELISA and RT-PCR. T cells exposed to 10 ug/ml nicotine during primary stimulation produced 2-fold more IFN-g during secondary responses compared with controls. Sustained IL-10 gene expression was noted even 4 days after primary stimulation in the presence of nicotine, which resulted in 30{\%} more IL-10 accumulation during secondary stimulation compared with controls. Exposure to ST during the primary T cell response led to significantly higher production of IL-2 (+270{\%}), IL-4 (+69{\%}), IFN-g (+300{\%}), and IL-10 (+73{\%}) during secondary responses without ST. These results indicate that exposure to ST or nicotine during the primary immune response will significantly influence cytokine production during the response of memory T cells.",
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