Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice

Joshua McWhirt, Monica Sathyanesan, Dayalan Sampath, Samuel Sathyanesan

Research output: Contribution to journalArticle

Abstract

The two strains of inbred mice, BALB/c and C57BL/6, are widely used in pre-clinical psychiatry research due to their differences in stress susceptibility. Gene profiling studies in these strains have implicated the inflammation pathway as the main contributor to these differences. We focused our attention on female mice and tested their response to 5- or 10-day exposure to restraint stress. We examined the stress induced changes in the regulation of 11 inflammatory cytokine genes and 12 glutamate receptor genes in the hippocampus of female BALB/c and C57BL/6 mice using quantitative PCR. Elevated proinflammatory cytokine genes include Tumor Necrosis Factor alpha (TNFa), nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), Interleukin 1 alpha (IL1a), Interleukin 1 receptor (IL1R), Interleukin 10 receptor alpha subunit (IL10Ra), Interleukin 10 receptor beta subunit (IL10Rb), and tumor necrosis factor (TNF)super family members. Our results show that BALB/c and C57BL/6 mice differ in the genes induced in response to stress exposure and the level of gene regulation change. Our results show that the gene regulation in female BALB/c and C57BL/6 mice differs between strains in the genes regulated and the magnitude of the changes.

Original languageEnglish (US)
Article number100169
JournalNeurobiology of Stress
Volume10
DOIs
StatePublished - Feb 1 2019

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Glutamate Receptors
Genes
Inflammation
Inbred C57BL Mouse
Gene expression
Interleukin-10 Receptor beta Subunit
Interleukin-10 Receptor alpha Subunit
Tumor Necrosis Factor-alpha
Cytokines
Interleukin-1alpha
Interleukin-1 Receptors
Inbred Strains Mice
Cells
Psychiatry
Hippocampus
B-Lymphocytes
Light
Polymerase Chain Reaction
Research

Keywords

  • Female
  • Gene expression
  • Glutamate receptors
  • Hippocampus
  • Proinflammatory genes
  • Restraint stress

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice. / McWhirt, Joshua; Sathyanesan, Monica; Sampath, Dayalan; Sathyanesan, Samuel.

In: Neurobiology of Stress, Vol. 10, 100169, 01.02.2019.

Research output: Contribution to journalArticle

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