Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat

Louis R. Lucas, Tina Dragisic, Caroline C. Duwaerts, Michael Swiatkowski, Hideo Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Previously, we have reported that brain regions that are thought to be involved in motivated behavior are altered in animals undergoing repeated exposures to immobilization stress. The goal of the present study was to determine the effects of recovery from this type of stress on these same mesolimbic brain regions. For this purpose, adult male Sprague-Dawley rats were initially exposed to immobilization stress either once (2. h) or repeatedly (2. h × 10. days). Rats were then either allowed to recover from the stressor for a shorter (2. days) or longer period of time (9. days) in their home cages. At the end of this recovery period, rats were euthanized and trunk blood and brains were processed for serum corticosterone (CORT) and neurochemistry, respectively. Brain mRNA levels were determined via in situ hybridization for the opioid preprodynorphin (DYN) and its cognate receptor (kappa, KOR), in striatal and accumbal subregions. A pattern of selective transcriptional activation emerged in the four resultant treatment conditions where a short recovery from either a single or repeated exposure to immobilization produced increases in KOR-mRNA levels in striatal and nucleus accumbens (Acb) subregions. Relative to controls, these differences were diminished after a longer recovery period. Interestingly, DYN-mRNA levels were unchanged after the shorter recovery period and after single or repeated immobilizations but appeared to be induced after a longer recovery period after repeated immobilizations. A relative amount of weight loss occurred after immobilization following repeated but not single exposure to stress. In addition, only those rats recovering from repeated stress exposures had higher CORT levels compared with non-immobilized controls. These results suggest that recovery from immobilization stress may alter the motivational system after as little as a single immobilization and that a possible dysphoric effect on appetitive behavior may be reflected by an altered striatal dynorphin system.

Original languageEnglish (US)
Pages (from-to)972-980
Number of pages9
JournalPhysiology and Behavior
Volume104
Issue number5
DOIs
StatePublished - Oct 24 2011

Fingerprint

Corpus Striatum
kappa Opioid Receptor
Immobilization
Messenger RNA
Brain
Corticosterone
Appetitive Behavior
Neurochemistry
Dynorphins
pre-prodynorphin
Nucleus Accumbens
Opioid Analgesics
Transcriptional Activation
In Situ Hybridization
Sprague Dawley Rats
Weight Loss

Keywords

  • Dopamine
  • Dysphoria
  • Nucleus accumbens
  • Opioids
  • Recovery
  • Stress

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat. / Lucas, Louis R.; Dragisic, Tina; Duwaerts, Caroline C.; Swiatkowski, Michael; Suzuki, Hideo.

In: Physiology and Behavior, Vol. 104, No. 5, 24.10.2011, p. 972-980.

Research output: Contribution to journalArticle

Lucas, Louis R. ; Dragisic, Tina ; Duwaerts, Caroline C. ; Swiatkowski, Michael ; Suzuki, Hideo. / Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat. In: Physiology and Behavior. 2011 ; Vol. 104, No. 5. pp. 972-980.
@article{7001f26c2186497ea34db17164deb86a,
title = "Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat",
abstract = "Previously, we have reported that brain regions that are thought to be involved in motivated behavior are altered in animals undergoing repeated exposures to immobilization stress. The goal of the present study was to determine the effects of recovery from this type of stress on these same mesolimbic brain regions. For this purpose, adult male Sprague-Dawley rats were initially exposed to immobilization stress either once (2. h) or repeatedly (2. h × 10. days). Rats were then either allowed to recover from the stressor for a shorter (2. days) or longer period of time (9. days) in their home cages. At the end of this recovery period, rats were euthanized and trunk blood and brains were processed for serum corticosterone (CORT) and neurochemistry, respectively. Brain mRNA levels were determined via in situ hybridization for the opioid preprodynorphin (DYN) and its cognate receptor (kappa, KOR), in striatal and accumbal subregions. A pattern of selective transcriptional activation emerged in the four resultant treatment conditions where a short recovery from either a single or repeated exposure to immobilization produced increases in KOR-mRNA levels in striatal and nucleus accumbens (Acb) subregions. Relative to controls, these differences were diminished after a longer recovery period. Interestingly, DYN-mRNA levels were unchanged after the shorter recovery period and after single or repeated immobilizations but appeared to be induced after a longer recovery period after repeated immobilizations. A relative amount of weight loss occurred after immobilization following repeated but not single exposure to stress. In addition, only those rats recovering from repeated stress exposures had higher CORT levels compared with non-immobilized controls. These results suggest that recovery from immobilization stress may alter the motivational system after as little as a single immobilization and that a possible dysphoric effect on appetitive behavior may be reflected by an altered striatal dynorphin system.",
keywords = "Dopamine, Dysphoria, Nucleus accumbens, Opioids, Recovery, Stress",
author = "Lucas, {Louis R.} and Tina Dragisic and Duwaerts, {Caroline C.} and Michael Swiatkowski and Hideo Suzuki",
year = "2011",
month = "10",
day = "24",
doi = "10.1016/j.physbeh.2011.06.017",
language = "English (US)",
volume = "104",
pages = "972--980",
journal = "Physiology and Behavior",
issn = "0031-9384",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Effects of recovery from immobilization stress on striatal preprodynorphin- and kappa opioid receptor-mRNA levels of the male rat

AU - Lucas, Louis R.

AU - Dragisic, Tina

AU - Duwaerts, Caroline C.

AU - Swiatkowski, Michael

AU - Suzuki, Hideo

PY - 2011/10/24

Y1 - 2011/10/24

N2 - Previously, we have reported that brain regions that are thought to be involved in motivated behavior are altered in animals undergoing repeated exposures to immobilization stress. The goal of the present study was to determine the effects of recovery from this type of stress on these same mesolimbic brain regions. For this purpose, adult male Sprague-Dawley rats were initially exposed to immobilization stress either once (2. h) or repeatedly (2. h × 10. days). Rats were then either allowed to recover from the stressor for a shorter (2. days) or longer period of time (9. days) in their home cages. At the end of this recovery period, rats were euthanized and trunk blood and brains were processed for serum corticosterone (CORT) and neurochemistry, respectively. Brain mRNA levels were determined via in situ hybridization for the opioid preprodynorphin (DYN) and its cognate receptor (kappa, KOR), in striatal and accumbal subregions. A pattern of selective transcriptional activation emerged in the four resultant treatment conditions where a short recovery from either a single or repeated exposure to immobilization produced increases in KOR-mRNA levels in striatal and nucleus accumbens (Acb) subregions. Relative to controls, these differences were diminished after a longer recovery period. Interestingly, DYN-mRNA levels were unchanged after the shorter recovery period and after single or repeated immobilizations but appeared to be induced after a longer recovery period after repeated immobilizations. A relative amount of weight loss occurred after immobilization following repeated but not single exposure to stress. In addition, only those rats recovering from repeated stress exposures had higher CORT levels compared with non-immobilized controls. These results suggest that recovery from immobilization stress may alter the motivational system after as little as a single immobilization and that a possible dysphoric effect on appetitive behavior may be reflected by an altered striatal dynorphin system.

AB - Previously, we have reported that brain regions that are thought to be involved in motivated behavior are altered in animals undergoing repeated exposures to immobilization stress. The goal of the present study was to determine the effects of recovery from this type of stress on these same mesolimbic brain regions. For this purpose, adult male Sprague-Dawley rats were initially exposed to immobilization stress either once (2. h) or repeatedly (2. h × 10. days). Rats were then either allowed to recover from the stressor for a shorter (2. days) or longer period of time (9. days) in their home cages. At the end of this recovery period, rats were euthanized and trunk blood and brains were processed for serum corticosterone (CORT) and neurochemistry, respectively. Brain mRNA levels were determined via in situ hybridization for the opioid preprodynorphin (DYN) and its cognate receptor (kappa, KOR), in striatal and accumbal subregions. A pattern of selective transcriptional activation emerged in the four resultant treatment conditions where a short recovery from either a single or repeated exposure to immobilization produced increases in KOR-mRNA levels in striatal and nucleus accumbens (Acb) subregions. Relative to controls, these differences were diminished after a longer recovery period. Interestingly, DYN-mRNA levels were unchanged after the shorter recovery period and after single or repeated immobilizations but appeared to be induced after a longer recovery period after repeated immobilizations. A relative amount of weight loss occurred after immobilization following repeated but not single exposure to stress. In addition, only those rats recovering from repeated stress exposures had higher CORT levels compared with non-immobilized controls. These results suggest that recovery from immobilization stress may alter the motivational system after as little as a single immobilization and that a possible dysphoric effect on appetitive behavior may be reflected by an altered striatal dynorphin system.

KW - Dopamine

KW - Dysphoria

KW - Nucleus accumbens

KW - Opioids

KW - Recovery

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=80053051700&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053051700&partnerID=8YFLogxK

U2 - 10.1016/j.physbeh.2011.06.017

DO - 10.1016/j.physbeh.2011.06.017

M3 - Article

VL - 104

SP - 972

EP - 980

JO - Physiology and Behavior

JF - Physiology and Behavior

SN - 0031-9384

IS - 5

ER -