Effects of protein aggregation in isocratic nonlinear chromatography

Roger D. Whitley, Kevin E. Van Cott, James A. Berninger, N. ‐H Linda Wang

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A versatile reaction‐separation (VERSE) model was developed to quantitatively simulate the behavior of chromatographic separations coupled with reactions. Detailed mass transfer and reaction mechanisms are considered. Aggregation data of myoglobin and β‐lactoglobulin A verified the model. The effects of concentration, equilibrium distribution, reaction rate, convection rate, particle radius, and relative affinity are shown for a dimerizing system. When the aggregation rate is relatively slow compared with convection and mass transfer rates, the individual forms behave as separate species in frontal, elution, and displacement chromatography. For rapid aggregation rates, the individual forms behave as a single component with an average affinity. The wave asymmetry and increased spreading due to aggregation depend on relative affinity differences. Serious error may result if aggregation is overlooked in parameter estimation using frontal or pulse analysis. The dimensionless group principles developed here are useful in scaling and predicting when peak or wave splitting or merging will occur in reaction chromatography systems.

Original languageEnglish (US)
Pages (from-to)555-568
Number of pages14
JournalAIChE Journal
Volume37
Issue number4
DOIs
StatePublished - Apr 1991

Fingerprint

Convection
Chromatography
Agglomeration
Proteins
Lactoglobulins
Myoglobin
Mass transfer
Merging
Parameter estimation
Reaction rates

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Chemical Engineering(all)

Cite this

Effects of protein aggregation in isocratic nonlinear chromatography. / Whitley, Roger D.; Van Cott, Kevin E.; Berninger, James A.; Wang, N. ‐H Linda.

In: AIChE Journal, Vol. 37, No. 4, 04.1991, p. 555-568.

Research output: Contribution to journalArticle

Whitley, Roger D. ; Van Cott, Kevin E. ; Berninger, James A. ; Wang, N. ‐H Linda. / Effects of protein aggregation in isocratic nonlinear chromatography. In: AIChE Journal. 1991 ; Vol. 37, No. 4. pp. 555-568.
@article{edddf0854e64462198b05dae80050a33,
title = "Effects of protein aggregation in isocratic nonlinear chromatography",
abstract = "A versatile reaction‐separation (VERSE) model was developed to quantitatively simulate the behavior of chromatographic separations coupled with reactions. Detailed mass transfer and reaction mechanisms are considered. Aggregation data of myoglobin and β‐lactoglobulin A verified the model. The effects of concentration, equilibrium distribution, reaction rate, convection rate, particle radius, and relative affinity are shown for a dimerizing system. When the aggregation rate is relatively slow compared with convection and mass transfer rates, the individual forms behave as separate species in frontal, elution, and displacement chromatography. For rapid aggregation rates, the individual forms behave as a single component with an average affinity. The wave asymmetry and increased spreading due to aggregation depend on relative affinity differences. Serious error may result if aggregation is overlooked in parameter estimation using frontal or pulse analysis. The dimensionless group principles developed here are useful in scaling and predicting when peak or wave splitting or merging will occur in reaction chromatography systems.",
author = "Whitley, {Roger D.} and {Van Cott}, {Kevin E.} and Berninger, {James A.} and Wang, {N. ‐H Linda}",
year = "1991",
month = "4",
doi = "10.1002/aic.690370409",
language = "English (US)",
volume = "37",
pages = "555--568",
journal = "AICHE Journal",
issn = "0001-1541",
publisher = "American Institute of Chemical Engineers",
number = "4",

}

TY - JOUR

T1 - Effects of protein aggregation in isocratic nonlinear chromatography

AU - Whitley, Roger D.

AU - Van Cott, Kevin E.

AU - Berninger, James A.

AU - Wang, N. ‐H Linda

PY - 1991/4

Y1 - 1991/4

N2 - A versatile reaction‐separation (VERSE) model was developed to quantitatively simulate the behavior of chromatographic separations coupled with reactions. Detailed mass transfer and reaction mechanisms are considered. Aggregation data of myoglobin and β‐lactoglobulin A verified the model. The effects of concentration, equilibrium distribution, reaction rate, convection rate, particle radius, and relative affinity are shown for a dimerizing system. When the aggregation rate is relatively slow compared with convection and mass transfer rates, the individual forms behave as separate species in frontal, elution, and displacement chromatography. For rapid aggregation rates, the individual forms behave as a single component with an average affinity. The wave asymmetry and increased spreading due to aggregation depend on relative affinity differences. Serious error may result if aggregation is overlooked in parameter estimation using frontal or pulse analysis. The dimensionless group principles developed here are useful in scaling and predicting when peak or wave splitting or merging will occur in reaction chromatography systems.

AB - A versatile reaction‐separation (VERSE) model was developed to quantitatively simulate the behavior of chromatographic separations coupled with reactions. Detailed mass transfer and reaction mechanisms are considered. Aggregation data of myoglobin and β‐lactoglobulin A verified the model. The effects of concentration, equilibrium distribution, reaction rate, convection rate, particle radius, and relative affinity are shown for a dimerizing system. When the aggregation rate is relatively slow compared with convection and mass transfer rates, the individual forms behave as separate species in frontal, elution, and displacement chromatography. For rapid aggregation rates, the individual forms behave as a single component with an average affinity. The wave asymmetry and increased spreading due to aggregation depend on relative affinity differences. Serious error may result if aggregation is overlooked in parameter estimation using frontal or pulse analysis. The dimensionless group principles developed here are useful in scaling and predicting when peak or wave splitting or merging will occur in reaction chromatography systems.

UR - http://www.scopus.com/inward/record.url?scp=0026138816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026138816&partnerID=8YFLogxK

U2 - 10.1002/aic.690370409

DO - 10.1002/aic.690370409

M3 - Article

AN - SCOPUS:0026138816

VL - 37

SP - 555

EP - 568

JO - AICHE Journal

JF - AICHE Journal

SN - 0001-1541

IS - 4

ER -