Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates

Ramesh Agarwal, Nidhi Sharma, Rama Chaudhry, Ashok Deorari, Vinod K. Paul, Ira H. Gewolb, Pinaki Panigrahi

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Background: Colonization patterns, especially by anaerobic flora, may play an important role in neonatal gut function. Probiotics could affect disease risk either directly through colonization or indirectly by promoting changes in gut microbial ecology. Methods: To study the ability of Lactobacillus GG (LGG) to colonize the neonatal gut and modify its microbial ecology, a prospective, randomized study was performed in 71 preterm infants of less than 2000 g birth weight. Infants less than 1500 g (24 treated, 15 control) received 10 9 LGG orally twice daily for 21 days. Those infants weighing 1500 to 1999 g (23 treated, 9 control) were treated for 8 days. Stools were collected before treatment and on day 7 to 8 (and day 14 and 21, in the infants weighing less than 1500 g) for quantitative aerobic and anaerobic cultures. Results: Colonization with LGG occurred in 5 of 24 (21%) infants who weighed less than 1500 g versus 11 of 23 (47%) in larger infants. Colonization was limited to infants who were not on antibiotics within 7 days of treatment with LGG. There was a paucity of bacterial species at baseline, although larger infants had more bacterial species (1.59 ± 0.13 (SEM) vs 1.11 ± 0.12; P < 0.03) and higher mean log colony forming units (CFU) (8.79 ± 0.43 vs 7.22 ± 0.63; P < 0.05) compared with infants weighing less than 1500 g LGG. Treatment in infants weighing less than 1500 g resulted in a significant increase in species number by day 7, with further increases by day 21. This increase was mainly the result of increased Gram (+) and anaerobic species. No difference in species number was noted in controls. Mean log CFU of Gram (-) bacteria did not change in treated infants weighing less than 1500 g. However, Gram (+) mean log CFU showed a significant increase on day 21 (6.1 ± 0.9) compared with day 0 (3.5 ± 0.9) (P < 0.05). No significant changes in species number or quantitative counts were noted after LGG treatment in the infants weighing 1500 to 1999 g LGG was well tolerated in all infants. Conclusion: The neonatal response to a probiotic preparation is dependent on gestational and post-natal age and prior antibiotic exposure. Although LGG is a relatively poor colonizer in infants, especially those infants weighing less than 1500 g at birth, it does appear to affect neonatal intestinal colonization patterns.

Original languageEnglish (US)
Pages (from-to)397-402
Number of pages6
JournalJournal of pediatric gastroenterology and nutrition
Volume36
Issue number3
DOIs
StatePublished - Mar 1 2003

Fingerprint

Lactobacillus rhamnosus
low birth weight
Low Birth Weight Infant
Lactobacillus
mouth
neonates
Newborn Infant
microorganisms
microbial ecology
probiotics
digestive system
antibiotics
Stem Cells
Probiotics
Ecology
interspecific variation
intestinal microorganisms
birth weight
Anti-Bacterial Agents
flora

Keywords

  • Colonization
  • Gut microflora
  • Lactobacillus GG
  • Probiotics

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

Cite this

Agarwal, R., Sharma, N., Chaudhry, R., Deorari, A., Paul, V. K., Gewolb, I. H., & Panigrahi, P. (2003). Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates. Journal of pediatric gastroenterology and nutrition, 36(3), 397-402. https://doi.org/10.1097/00005176-200303000-00019

Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates. / Agarwal, Ramesh; Sharma, Nidhi; Chaudhry, Rama; Deorari, Ashok; Paul, Vinod K.; Gewolb, Ira H.; Panigrahi, Pinaki.

In: Journal of pediatric gastroenterology and nutrition, Vol. 36, No. 3, 01.03.2003, p. 397-402.

Research output: Contribution to journalArticle

Agarwal, R, Sharma, N, Chaudhry, R, Deorari, A, Paul, VK, Gewolb, IH & Panigrahi, P 2003, 'Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates', Journal of pediatric gastroenterology and nutrition, vol. 36, no. 3, pp. 397-402. https://doi.org/10.1097/00005176-200303000-00019
Agarwal, Ramesh ; Sharma, Nidhi ; Chaudhry, Rama ; Deorari, Ashok ; Paul, Vinod K. ; Gewolb, Ira H. ; Panigrahi, Pinaki. / Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates. In: Journal of pediatric gastroenterology and nutrition. 2003 ; Vol. 36, No. 3. pp. 397-402.
@article{d3fc4bd3a4184a559764e277cf8de9c6,
title = "Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates",
abstract = "Background: Colonization patterns, especially by anaerobic flora, may play an important role in neonatal gut function. Probiotics could affect disease risk either directly through colonization or indirectly by promoting changes in gut microbial ecology. Methods: To study the ability of Lactobacillus GG (LGG) to colonize the neonatal gut and modify its microbial ecology, a prospective, randomized study was performed in 71 preterm infants of less than 2000 g birth weight. Infants less than 1500 g (24 treated, 15 control) received 10 9 LGG orally twice daily for 21 days. Those infants weighing 1500 to 1999 g (23 treated, 9 control) were treated for 8 days. Stools were collected before treatment and on day 7 to 8 (and day 14 and 21, in the infants weighing less than 1500 g) for quantitative aerobic and anaerobic cultures. Results: Colonization with LGG occurred in 5 of 24 (21{\%}) infants who weighed less than 1500 g versus 11 of 23 (47{\%}) in larger infants. Colonization was limited to infants who were not on antibiotics within 7 days of treatment with LGG. There was a paucity of bacterial species at baseline, although larger infants had more bacterial species (1.59 ± 0.13 (SEM) vs 1.11 ± 0.12; P < 0.03) and higher mean log colony forming units (CFU) (8.79 ± 0.43 vs 7.22 ± 0.63; P < 0.05) compared with infants weighing less than 1500 g LGG. Treatment in infants weighing less than 1500 g resulted in a significant increase in species number by day 7, with further increases by day 21. This increase was mainly the result of increased Gram (+) and anaerobic species. No difference in species number was noted in controls. Mean log CFU of Gram (-) bacteria did not change in treated infants weighing less than 1500 g. However, Gram (+) mean log CFU showed a significant increase on day 21 (6.1 ± 0.9) compared with day 0 (3.5 ± 0.9) (P < 0.05). No significant changes in species number or quantitative counts were noted after LGG treatment in the infants weighing 1500 to 1999 g LGG was well tolerated in all infants. Conclusion: The neonatal response to a probiotic preparation is dependent on gestational and post-natal age and prior antibiotic exposure. Although LGG is a relatively poor colonizer in infants, especially those infants weighing less than 1500 g at birth, it does appear to affect neonatal intestinal colonization patterns.",
keywords = "Colonization, Gut microflora, Lactobacillus GG, Probiotics",
author = "Ramesh Agarwal and Nidhi Sharma and Rama Chaudhry and Ashok Deorari and Paul, {Vinod K.} and Gewolb, {Ira H.} and Pinaki Panigrahi",
year = "2003",
month = "3",
day = "1",
doi = "10.1097/00005176-200303000-00019",
language = "English (US)",
volume = "36",
pages = "397--402",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
issn = "0277-2116",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Effects of oral Lactobacillus GG on enteric microflora in low-birth-weight neonates

AU - Agarwal, Ramesh

AU - Sharma, Nidhi

AU - Chaudhry, Rama

AU - Deorari, Ashok

AU - Paul, Vinod K.

AU - Gewolb, Ira H.

AU - Panigrahi, Pinaki

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Background: Colonization patterns, especially by anaerobic flora, may play an important role in neonatal gut function. Probiotics could affect disease risk either directly through colonization or indirectly by promoting changes in gut microbial ecology. Methods: To study the ability of Lactobacillus GG (LGG) to colonize the neonatal gut and modify its microbial ecology, a prospective, randomized study was performed in 71 preterm infants of less than 2000 g birth weight. Infants less than 1500 g (24 treated, 15 control) received 10 9 LGG orally twice daily for 21 days. Those infants weighing 1500 to 1999 g (23 treated, 9 control) were treated for 8 days. Stools were collected before treatment and on day 7 to 8 (and day 14 and 21, in the infants weighing less than 1500 g) for quantitative aerobic and anaerobic cultures. Results: Colonization with LGG occurred in 5 of 24 (21%) infants who weighed less than 1500 g versus 11 of 23 (47%) in larger infants. Colonization was limited to infants who were not on antibiotics within 7 days of treatment with LGG. There was a paucity of bacterial species at baseline, although larger infants had more bacterial species (1.59 ± 0.13 (SEM) vs 1.11 ± 0.12; P < 0.03) and higher mean log colony forming units (CFU) (8.79 ± 0.43 vs 7.22 ± 0.63; P < 0.05) compared with infants weighing less than 1500 g LGG. Treatment in infants weighing less than 1500 g resulted in a significant increase in species number by day 7, with further increases by day 21. This increase was mainly the result of increased Gram (+) and anaerobic species. No difference in species number was noted in controls. Mean log CFU of Gram (-) bacteria did not change in treated infants weighing less than 1500 g. However, Gram (+) mean log CFU showed a significant increase on day 21 (6.1 ± 0.9) compared with day 0 (3.5 ± 0.9) (P < 0.05). No significant changes in species number or quantitative counts were noted after LGG treatment in the infants weighing 1500 to 1999 g LGG was well tolerated in all infants. Conclusion: The neonatal response to a probiotic preparation is dependent on gestational and post-natal age and prior antibiotic exposure. Although LGG is a relatively poor colonizer in infants, especially those infants weighing less than 1500 g at birth, it does appear to affect neonatal intestinal colonization patterns.

AB - Background: Colonization patterns, especially by anaerobic flora, may play an important role in neonatal gut function. Probiotics could affect disease risk either directly through colonization or indirectly by promoting changes in gut microbial ecology. Methods: To study the ability of Lactobacillus GG (LGG) to colonize the neonatal gut and modify its microbial ecology, a prospective, randomized study was performed in 71 preterm infants of less than 2000 g birth weight. Infants less than 1500 g (24 treated, 15 control) received 10 9 LGG orally twice daily for 21 days. Those infants weighing 1500 to 1999 g (23 treated, 9 control) were treated for 8 days. Stools were collected before treatment and on day 7 to 8 (and day 14 and 21, in the infants weighing less than 1500 g) for quantitative aerobic and anaerobic cultures. Results: Colonization with LGG occurred in 5 of 24 (21%) infants who weighed less than 1500 g versus 11 of 23 (47%) in larger infants. Colonization was limited to infants who were not on antibiotics within 7 days of treatment with LGG. There was a paucity of bacterial species at baseline, although larger infants had more bacterial species (1.59 ± 0.13 (SEM) vs 1.11 ± 0.12; P < 0.03) and higher mean log colony forming units (CFU) (8.79 ± 0.43 vs 7.22 ± 0.63; P < 0.05) compared with infants weighing less than 1500 g LGG. Treatment in infants weighing less than 1500 g resulted in a significant increase in species number by day 7, with further increases by day 21. This increase was mainly the result of increased Gram (+) and anaerobic species. No difference in species number was noted in controls. Mean log CFU of Gram (-) bacteria did not change in treated infants weighing less than 1500 g. However, Gram (+) mean log CFU showed a significant increase on day 21 (6.1 ± 0.9) compared with day 0 (3.5 ± 0.9) (P < 0.05). No significant changes in species number or quantitative counts were noted after LGG treatment in the infants weighing 1500 to 1999 g LGG was well tolerated in all infants. Conclusion: The neonatal response to a probiotic preparation is dependent on gestational and post-natal age and prior antibiotic exposure. Although LGG is a relatively poor colonizer in infants, especially those infants weighing less than 1500 g at birth, it does appear to affect neonatal intestinal colonization patterns.

KW - Colonization

KW - Gut microflora

KW - Lactobacillus GG

KW - Probiotics

UR - http://www.scopus.com/inward/record.url?scp=0043135175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0043135175&partnerID=8YFLogxK

U2 - 10.1097/00005176-200303000-00019

DO - 10.1097/00005176-200303000-00019

M3 - Article

C2 - 12604982

AN - SCOPUS:0043135175

VL - 36

SP - 397

EP - 402

JO - Journal of Pediatric Gastroenterology and Nutrition

JF - Journal of Pediatric Gastroenterology and Nutrition

SN - 0277-2116

IS - 3

ER -