Effects of N,N-dimethylformamide and extracellular matrix on transforming growth factor-β binding to a human colon carcinoma cell line

A. E. Levine, B. Black, M. G. Brattain

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Alterations in the binding of transforming growth factor-β (TGF-β) to the MOSER human colon carcinoma cell line caused by N,N-dimethylformamide (DMF) or extracellular matrix (ECM) were examined. DMF induced a more differentiated phenotype in the MOSER cells and resulted in a twofold increase in TGF-β binding to the cells. This was due to an increase in receptor number with no significant alteration in the K(D). The extent of increased TGF-β binding was dependent on the dose and time of exposure to DMF. Upon removal of DMF, the receptor level returned to that of untreated cells within 6 hr. The binding of TGF-β1 and TGF-β2 to the cells was increased equally. Despite this increase in TGF-β binding in the presence of DMF, the sensitivity of the MOSER cells to the growth inhibitory effects of TGF-β was unaltered. Growth of the MOSER cells on ECM derived from a well-differentiated colon cell line increased the TGF-β receptor number twofold without altering the K(D). No change was observed if the MOSER cells were grown on ECM derived from a poorly differentiated cell line. While no alteration in sensitivity to TGF-β was observed on cells grown in the presence of DMF, MOSER cells grown on the ECDM derived from well-differentiated colon carcinoma cell lines were twofold more sensitive to the growth inhibitory effects of TGF-β. These results indicated that growth conditions which resulted in a more differentiated phenotype resulted in an increase in the cellular receptors for TGF-β.

Original languageEnglish (US)
Pages (from-to)459-466
Number of pages8
JournalJournal of Cellular Physiology
Volume138
Issue number3
StatePublished - 1989
Externally publishedYes

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Dimethylformamide
Transforming Growth Factors
Extracellular Matrix
Colon
Cells
Carcinoma
Cell Line
Growth
Phenotype
Growth Factor Receptors

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Effects of N,N-dimethylformamide and extracellular matrix on transforming growth factor-β binding to a human colon carcinoma cell line. / Levine, A. E.; Black, B.; Brattain, M. G.

In: Journal of Cellular Physiology, Vol. 138, No. 3, 1989, p. 459-466.

Research output: Contribution to journalArticle

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