Effects of nicotine on bone mass, turnover, and strength in adult female rats

U. T. Iwaniec, Y. K. Fung, M. P. Akhter, M. C. Haven, S. Nespor, Gleb Haynatzki, D. M. Cullen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

This study investigated the effects of nicotine, the chemical responsible for tobacco addiction, on bone and on serum mineral and calcitropic hormone levels in adult, female rats to help resolve a current controversy regarding the impact of nicotine on bone health. Seven-month-old rats received either saline (n = 12), low-dose nicotine (4.5 mg/kg/day, n = 2), or high-dose nicotine (6.0 mg/kg/day, n = 12) administered subcutaneously via osmotic minipumps for 3 months. Blood, femora, tibiae, and lumbar vertebrae (3-5) were collected at necropsy for determination of serum mineral and hormonal concentrations, bone density (femora and vertebrae), bone turnover (tibiae), and bone strength (femora). The presence of nicotine in serum (111 ± 7 and 137 ± 10 ng/ml for the low- and high-dose nicotine groups, respectively) confirmed successful delivery of the drug via osmotic minipumps. Nicotine-induced treatment differences were not detected in serum calcium, 25-hydroxyvitamin D, and 1,25-dihydroxyvitamin D. However, serum phosphorus and parathyroid hormone (PTH) were higher in rats treated with high-dose nicotine, and serum calcitonin was lower in rats treated with both high- and low-dose nicotine than in control rats. Nicotine treatment had no effect on tibial cancellous or cortical bone turnover or femoral bone mineral content (BMC) and density (BMD). Femoral ultimate load and vertebral BMC were lower in rats treated with high-dose nicotine than in control rats. We conclude that nicotine at serum concentrations 2.5-fold greater than the average in smokers has limited detrimental effects on bone in normal, healthy female rats.

Original languageEnglish (US)
Pages (from-to)358-364
Number of pages7
JournalCalcified Tissue International
Volume68
Issue number6
DOIs
StatePublished - Jul 18 2001

Fingerprint

Bone Remodeling
Nicotine
Bone Density
Serum
Femur
Bone and Bones
Thigh
Tibia
Minerals
Lumbar Vertebrae
Calcitonin
Parathyroid Hormone
Phosphorus
Tobacco
Spine
Hormones

Keywords

  • Bone remodeling
  • Smoking
  • Tobacco

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Iwaniec, U. T., Fung, Y. K., Akhter, M. P., Haven, M. C., Nespor, S., Haynatzki, G., & Cullen, D. M. (2001). Effects of nicotine on bone mass, turnover, and strength in adult female rats. Calcified Tissue International, 68(6), 358-364. https://doi.org/10.1007/s00223-001-0011-8

Effects of nicotine on bone mass, turnover, and strength in adult female rats. / Iwaniec, U. T.; Fung, Y. K.; Akhter, M. P.; Haven, M. C.; Nespor, S.; Haynatzki, Gleb; Cullen, D. M.

In: Calcified Tissue International, Vol. 68, No. 6, 18.07.2001, p. 358-364.

Research output: Contribution to journalArticle

Iwaniec, UT, Fung, YK, Akhter, MP, Haven, MC, Nespor, S, Haynatzki, G & Cullen, DM 2001, 'Effects of nicotine on bone mass, turnover, and strength in adult female rats', Calcified Tissue International, vol. 68, no. 6, pp. 358-364. https://doi.org/10.1007/s00223-001-0011-8
Iwaniec, U. T. ; Fung, Y. K. ; Akhter, M. P. ; Haven, M. C. ; Nespor, S. ; Haynatzki, Gleb ; Cullen, D. M. / Effects of nicotine on bone mass, turnover, and strength in adult female rats. In: Calcified Tissue International. 2001 ; Vol. 68, No. 6. pp. 358-364.
@article{40a9f2d02c0147f4a1a7b32e3e2c4d93,
title = "Effects of nicotine on bone mass, turnover, and strength in adult female rats",
abstract = "This study investigated the effects of nicotine, the chemical responsible for tobacco addiction, on bone and on serum mineral and calcitropic hormone levels in adult, female rats to help resolve a current controversy regarding the impact of nicotine on bone health. Seven-month-old rats received either saline (n = 12), low-dose nicotine (4.5 mg/kg/day, n = 2), or high-dose nicotine (6.0 mg/kg/day, n = 12) administered subcutaneously via osmotic minipumps for 3 months. Blood, femora, tibiae, and lumbar vertebrae (3-5) were collected at necropsy for determination of serum mineral and hormonal concentrations, bone density (femora and vertebrae), bone turnover (tibiae), and bone strength (femora). The presence of nicotine in serum (111 ± 7 and 137 ± 10 ng/ml for the low- and high-dose nicotine groups, respectively) confirmed successful delivery of the drug via osmotic minipumps. Nicotine-induced treatment differences were not detected in serum calcium, 25-hydroxyvitamin D, and 1,25-dihydroxyvitamin D. However, serum phosphorus and parathyroid hormone (PTH) were higher in rats treated with high-dose nicotine, and serum calcitonin was lower in rats treated with both high- and low-dose nicotine than in control rats. Nicotine treatment had no effect on tibial cancellous or cortical bone turnover or femoral bone mineral content (BMC) and density (BMD). Femoral ultimate load and vertebral BMC were lower in rats treated with high-dose nicotine than in control rats. We conclude that nicotine at serum concentrations 2.5-fold greater than the average in smokers has limited detrimental effects on bone in normal, healthy female rats.",
keywords = "Bone remodeling, Smoking, Tobacco",
author = "Iwaniec, {U. T.} and Fung, {Y. K.} and Akhter, {M. P.} and Haven, {M. C.} and S. Nespor and Gleb Haynatzki and Cullen, {D. M.}",
year = "2001",
month = "7",
day = "18",
doi = "10.1007/s00223-001-0011-8",
language = "English (US)",
volume = "68",
pages = "358--364",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Effects of nicotine on bone mass, turnover, and strength in adult female rats

AU - Iwaniec, U. T.

AU - Fung, Y. K.

AU - Akhter, M. P.

AU - Haven, M. C.

AU - Nespor, S.

AU - Haynatzki, Gleb

AU - Cullen, D. M.

PY - 2001/7/18

Y1 - 2001/7/18

N2 - This study investigated the effects of nicotine, the chemical responsible for tobacco addiction, on bone and on serum mineral and calcitropic hormone levels in adult, female rats to help resolve a current controversy regarding the impact of nicotine on bone health. Seven-month-old rats received either saline (n = 12), low-dose nicotine (4.5 mg/kg/day, n = 2), or high-dose nicotine (6.0 mg/kg/day, n = 12) administered subcutaneously via osmotic minipumps for 3 months. Blood, femora, tibiae, and lumbar vertebrae (3-5) were collected at necropsy for determination of serum mineral and hormonal concentrations, bone density (femora and vertebrae), bone turnover (tibiae), and bone strength (femora). The presence of nicotine in serum (111 ± 7 and 137 ± 10 ng/ml for the low- and high-dose nicotine groups, respectively) confirmed successful delivery of the drug via osmotic minipumps. Nicotine-induced treatment differences were not detected in serum calcium, 25-hydroxyvitamin D, and 1,25-dihydroxyvitamin D. However, serum phosphorus and parathyroid hormone (PTH) were higher in rats treated with high-dose nicotine, and serum calcitonin was lower in rats treated with both high- and low-dose nicotine than in control rats. Nicotine treatment had no effect on tibial cancellous or cortical bone turnover or femoral bone mineral content (BMC) and density (BMD). Femoral ultimate load and vertebral BMC were lower in rats treated with high-dose nicotine than in control rats. We conclude that nicotine at serum concentrations 2.5-fold greater than the average in smokers has limited detrimental effects on bone in normal, healthy female rats.

AB - This study investigated the effects of nicotine, the chemical responsible for tobacco addiction, on bone and on serum mineral and calcitropic hormone levels in adult, female rats to help resolve a current controversy regarding the impact of nicotine on bone health. Seven-month-old rats received either saline (n = 12), low-dose nicotine (4.5 mg/kg/day, n = 2), or high-dose nicotine (6.0 mg/kg/day, n = 12) administered subcutaneously via osmotic minipumps for 3 months. Blood, femora, tibiae, and lumbar vertebrae (3-5) were collected at necropsy for determination of serum mineral and hormonal concentrations, bone density (femora and vertebrae), bone turnover (tibiae), and bone strength (femora). The presence of nicotine in serum (111 ± 7 and 137 ± 10 ng/ml for the low- and high-dose nicotine groups, respectively) confirmed successful delivery of the drug via osmotic minipumps. Nicotine-induced treatment differences were not detected in serum calcium, 25-hydroxyvitamin D, and 1,25-dihydroxyvitamin D. However, serum phosphorus and parathyroid hormone (PTH) were higher in rats treated with high-dose nicotine, and serum calcitonin was lower in rats treated with both high- and low-dose nicotine than in control rats. Nicotine treatment had no effect on tibial cancellous or cortical bone turnover or femoral bone mineral content (BMC) and density (BMD). Femoral ultimate load and vertebral BMC were lower in rats treated with high-dose nicotine than in control rats. We conclude that nicotine at serum concentrations 2.5-fold greater than the average in smokers has limited detrimental effects on bone in normal, healthy female rats.

KW - Bone remodeling

KW - Smoking

KW - Tobacco

UR - http://www.scopus.com/inward/record.url?scp=0034940342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034940342&partnerID=8YFLogxK

U2 - 10.1007/s00223-001-0011-8

DO - 10.1007/s00223-001-0011-8

M3 - Article

VL - 68

SP - 358

EP - 364

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 6

ER -