Effects of Moraxella (Branhamella) ovis culture filtrates on bovine erythrocytes, peripheral mononuclear cells, and corneal epithelial cells

Henry E. Cerny, Douglas G. Rogers, Jeffrey T. Gray, David R. Smith, Susanne Hinkley

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Infectious bovine keratoconjunctivitis (IBK) is a highly contagious ocular disease that affects cattle of all ages and that occurs worldwide. Piliated hemolytic Moraxella bovis is recognized as the etiologic agent of IBK. According to data from the Nebraska Veterinary Diagnostic Laboratory System, however, Moraxella (Branhamella) ovis has been isolated with increasing frequency from cattle affected with IBK. The objective of this study was, therefore, to examine M. ovis field isolates for the presence of the putative virulence factors of M. bovis. Culture filtrates from selected M. ovis field isolates demonstrated hemolytic activity on bovine erythrocytes and cytotoxic activity on bovine peripheral blood mononuclear cells and corneal epithelial cells. The hemolytic activity of the culture filtrates was attenuated after heat treatment. Polyclonal antibodies raised against the M. bovis hemolysin-cytotoxin also recognized a protein of approximately 98 kDa in a Western blot assay. These data indicate that the M. ovis field isolates examined produce one or more heat-labile exotoxins and may suggest that M. ovis plays a role in the pathogenesis of IBK.

Original languageEnglish (US)
Pages (from-to)772-776
Number of pages5
JournalJournal of clinical microbiology
Volume44
Issue number3
DOIs
StatePublished - Mar 1 2006

Fingerprint

Moraxella
Sheep
Infectious Keratoconjunctivitis
Erythrocytes
Epithelial Cells
Moraxella (Moraxella) bovis
Hot Temperature
Exotoxins
Hemolysin Proteins
Eye Diseases
Cytotoxins
Virulence Factors
Blood Cells
Western Blotting

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Effects of Moraxella (Branhamella) ovis culture filtrates on bovine erythrocytes, peripheral mononuclear cells, and corneal epithelial cells. / Cerny, Henry E.; Rogers, Douglas G.; Gray, Jeffrey T.; Smith, David R.; Hinkley, Susanne.

In: Journal of clinical microbiology, Vol. 44, No. 3, 01.03.2006, p. 772-776.

Research output: Contribution to journalArticle

Cerny, Henry E. ; Rogers, Douglas G. ; Gray, Jeffrey T. ; Smith, David R. ; Hinkley, Susanne. / Effects of Moraxella (Branhamella) ovis culture filtrates on bovine erythrocytes, peripheral mononuclear cells, and corneal epithelial cells. In: Journal of clinical microbiology. 2006 ; Vol. 44, No. 3. pp. 772-776.
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