Effects of mitomycin on human colon carcinoma cells

Byron H. Long, J. K.V. Wiiison, D. E. Brattain, Steven Muslal, M. G. Brattain

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Subpopulations of malignant cells from primary cultures of human colon carcinoma were characterized with respect to their response to mitomycin (MMC). Growth inhibition assays indicated values of 2.06, 0.93, and 0.33 µM for the concentration of drug giving 50% inhibition of growth for sublines HCT 116b, HCT 116, and HCT 116a, respectively. Alkaline elution of filter-bound DNA from cells exposed to MMC in vitro showed a positive correlation between the amount of DNA cross-linking and growth inhibition as a function of drug concentration. Comparable DNA cross-linking was obtained at MMC concentrations of 10 µM for HCT 116b and 5 µM and HCT 116. The cross-linking of DNA from HCT 116a cells at 5 µM MMC was approximately equal to that from HCT 116 cells at doses between 10 and 20 µM MMC. Cross-link removal as a function of time after drug removal of MMC-treated cells was also measured. There was little difference in the rates of alkaline DNA elution after drug removal between HCT 116b and HCT 116a, suggesting that the ability to repair cross-links was not responsible for the differential sensitivities of the cells to MMC. The relative sensitivities of the subpopulations to MMC were reflected in vivo by MMC treatment of nude BALB/c mice bearing xenografts of the cultured sub-lines.

Original languageEnglish (US)
Pages (from-to)787-792
Number of pages6
JournalJournal of the National Cancer Institute
Volume73
Issue number4
DOIs
StatePublished - Jan 1 1984

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Mitomycin
Colon
Carcinoma
DNA
Pharmaceutical Preparations
Growth
HCT116 Cells
Primary Cell Culture
Heterografts

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Long, B. H., Wiiison, J. K. V., Brattain, D. E., Muslal, S., & Brattain, M. G. (1984). Effects of mitomycin on human colon carcinoma cells. Journal of the National Cancer Institute, 73(4), 787-792. https://doi.org/10.1093/jnci/73.4.787

Effects of mitomycin on human colon carcinoma cells. / Long, Byron H.; Wiiison, J. K.V.; Brattain, D. E.; Muslal, Steven; Brattain, M. G.

In: Journal of the National Cancer Institute, Vol. 73, No. 4, 01.01.1984, p. 787-792.

Research output: Contribution to journalArticle

Long, BH, Wiiison, JKV, Brattain, DE, Muslal, S & Brattain, MG 1984, 'Effects of mitomycin on human colon carcinoma cells', Journal of the National Cancer Institute, vol. 73, no. 4, pp. 787-792. https://doi.org/10.1093/jnci/73.4.787
Long BH, Wiiison JKV, Brattain DE, Muslal S, Brattain MG. Effects of mitomycin on human colon carcinoma cells. Journal of the National Cancer Institute. 1984 Jan 1;73(4):787-792. https://doi.org/10.1093/jnci/73.4.787
Long, Byron H. ; Wiiison, J. K.V. ; Brattain, D. E. ; Muslal, Steven ; Brattain, M. G. / Effects of mitomycin on human colon carcinoma cells. In: Journal of the National Cancer Institute. 1984 ; Vol. 73, No. 4. pp. 787-792.
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