Effects of lipopolysaccharide on gastric stasis: Role of cyclooxygenase

Sonlee D. West, James W. Suliburk, Gregory S. Smith, David W. Mercer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.

Original languageEnglish (US)
Pages (from-to)754-765
Number of pages12
JournalDigestive Diseases and Sciences
Volume51
Issue number4
DOIs
StatePublished - Apr 1 2006

Fingerprint

Gastroparesis
Prostaglandin-Endoperoxide Synthases
Lipopolysaccharides
Cyclooxygenase 2
Stomach
Cyclooxygenase 1
Salicylates
Prostaglandins
Lasers
Ethanol

Keywords

  • Gastroprotection
  • Lipopolysaccharide
  • NS-398
  • SC-560
  • Salicylate

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Effects of lipopolysaccharide on gastric stasis : Role of cyclooxygenase. / West, Sonlee D.; Suliburk, James W.; Smith, Gregory S.; Mercer, David W.

In: Digestive Diseases and Sciences, Vol. 51, No. 4, 01.04.2006, p. 754-765.

Research output: Contribution to journalArticle

West, Sonlee D. ; Suliburk, James W. ; Smith, Gregory S. ; Mercer, David W. / Effects of lipopolysaccharide on gastric stasis : Role of cyclooxygenase. In: Digestive Diseases and Sciences. 2006 ; Vol. 51, No. 4. pp. 754-765.
@article{fb1e2ad4711d4b4ca5dd9a35550cd605,
title = "Effects of lipopolysaccharide on gastric stasis: Role of cyclooxygenase",
abstract = "This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.",
keywords = "Gastroprotection, Lipopolysaccharide, NS-398, SC-560, Salicylate",
author = "West, {Sonlee D.} and Suliburk, {James W.} and Smith, {Gregory S.} and Mercer, {David W.}",
year = "2006",
month = "4",
day = "1",
doi = "10.1007/s10620-006-3203-2",
language = "English (US)",
volume = "51",
pages = "754--765",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Effects of lipopolysaccharide on gastric stasis

T2 - Role of cyclooxygenase

AU - West, Sonlee D.

AU - Suliburk, James W.

AU - Smith, Gregory S.

AU - Mercer, David W.

PY - 2006/4/1

Y1 - 2006/4/1

N2 - This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.

AB - This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.

KW - Gastroprotection

KW - Lipopolysaccharide

KW - NS-398

KW - SC-560

KW - Salicylate

UR - http://www.scopus.com/inward/record.url?scp=33645843917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645843917&partnerID=8YFLogxK

U2 - 10.1007/s10620-006-3203-2

DO - 10.1007/s10620-006-3203-2

M3 - Article

C2 - 16615000

AN - SCOPUS:33645843917

VL - 51

SP - 754

EP - 765

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 4

ER -