Abstract
This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 754-765 |
| Number of pages | 12 |
| Journal | Digestive Diseases and Sciences |
| Volume | 51 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2006 |
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Keywords
- Gastroprotection
- Lipopolysaccharide
- NS-398
- SC-560
- Salicylate
ASJC Scopus subject areas
- Physiology
- Gastroenterology
Cite this
Effects of lipopolysaccharide on gastric stasis : Role of cyclooxygenase. / West, Sonlee D.; Suliburk, James W.; Smith, Gregory S.; Mercer, David W.
In: Digestive Diseases and Sciences, Vol. 51, No. 4, 01.04.2006, p. 754-765.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of lipopolysaccharide on gastric stasis
T2 - Role of cyclooxygenase
AU - West, Sonlee D.
AU - Suliburk, James W.
AU - Smith, Gregory S.
AU - Mercer, David W.
PY - 2006/4/1
Y1 - 2006/4/1
N2 - This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.
AB - This study was done to examine the role of cyclooxygenase (COX) in lipopolysaccharide (LPS)-induced gastroprotection and gastric stasis. In conscious rats, LPS dose and time dependently increased gastric luminal fluid accumulation. LPS decreased blood flow (laser Doppler) and prevented gastric injury from acidified ethanol at time points before significant fluid accumulation occurred. LPS increased COX-2 but not COX-1 expression. In contrast, LPS decreased gastric mucosal prostaglandin synthesis. LPS-induced gastric luminal fluid accumulation was negated by both nonselective COX inhibition with salicylate and selective COX-2 inhibition with NS-398 but not by selective COX-1 inhibition with SC-560. Neither salicylate nor NS-398 blocked LPS-induced gastroprotection. LPS-induced gastroprotection does not depend entirely on accumulation of luminal fluid and is independent of COX-1 and COX-2. However, the ability of LPS to cause gastric stasis and increase gastric luminal fluid accumulation involves COX-2.
KW - Gastroprotection
KW - Lipopolysaccharide
KW - NS-398
KW - SC-560
KW - Salicylate
UR - http://www.scopus.com/inward/record.url?scp=33645843917&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645843917&partnerID=8YFLogxK
U2 - 10.1007/s10620-006-3203-2
DO - 10.1007/s10620-006-3203-2
M3 - Article
C2 - 16615000
AN - SCOPUS:33645843917
VL - 51
SP - 754
EP - 765
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 4
ER -