Effects of lipid-binding proteins APO A-I, APO A-II, β2-glycoprotein I, and C-reactive protein on activation of factor X by tissue factor - factor VIIa

Steven D. Carson, Sherman E. Ross

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tissue factor is the membrane-associated protein which mediates activation of factors IX and X by factor VII. In a purified, reconstituted bovine system, factor X activation by the tissue factor-factor VIIa complex is inhibited by the mixed apoproteins from human high density lipoprotein (HDL) and by isolated apolipoprotein A-II (apo A-II). Other proteins found associated with plasma lipoproteins, apolipoprotein A-I (apo A-I), C-reactive protein (CRP), and β2-glycoprotein I (β2GPI), have been examined for effects on the activation of factor X by tissue factor-factor VIIa. In these experiments, bovine tissue factor, reconstituted into phosphatidylserine-phosphatidylcholine (PS/PC; 30 70) vesicles, was used at a single concentration while factor X (the substrate), factor VIIa (the enzyme), and the potentially inhibitory proteins were varied in a continuous chromogenic assay. Apo A-II and CRP clearly inhibit tissue factor-factor VIIa activation of factor X, while apo A-I and β2GPI have little or no effect. These results demonstrate that different lipid binding proteins vary in their effects on tissue factor activity.

Original languageEnglish (US)
Pages (from-to)669-678
Number of pages10
JournalThrombosis Research
Volume50
Issue number5
DOIs
StatePublished - Jun 1 1988

Fingerprint

Factor VIIa
Factor X
Thromboplastin
C-Reactive Protein
Carrier Proteins
Apolipoprotein A-II
Lipids
Apolipoprotein A-I
Glycoproteins
Apolipoproteins C
Factor VII
Factor IX
Apoproteins
Phosphatidylserines
HDL Lipoproteins
Phosphatidylcholines
glycoprotein gC, herpes simplex virus type 1
hexachlorocyclohexane x-factor
Membrane Proteins
Proteins

Keywords

  • Tissue factor
  • apolipoproteins
  • inhibitors

ASJC Scopus subject areas

  • Hematology

Cite this

@article{636555815631406b9b27b86a0e076944,
title = "Effects of lipid-binding proteins APO A-I, APO A-II, β2-glycoprotein I, and C-reactive protein on activation of factor X by tissue factor - factor VIIa",
abstract = "Tissue factor is the membrane-associated protein which mediates activation of factors IX and X by factor VII. In a purified, reconstituted bovine system, factor X activation by the tissue factor-factor VIIa complex is inhibited by the mixed apoproteins from human high density lipoprotein (HDL) and by isolated apolipoprotein A-II (apo A-II). Other proteins found associated with plasma lipoproteins, apolipoprotein A-I (apo A-I), C-reactive protein (CRP), and β2-glycoprotein I (β2GPI), have been examined for effects on the activation of factor X by tissue factor-factor VIIa. In these experiments, bovine tissue factor, reconstituted into phosphatidylserine-phosphatidylcholine (PS/PC; 30 70) vesicles, was used at a single concentration while factor X (the substrate), factor VIIa (the enzyme), and the potentially inhibitory proteins were varied in a continuous chromogenic assay. Apo A-II and CRP clearly inhibit tissue factor-factor VIIa activation of factor X, while apo A-I and β2GPI have little or no effect. These results demonstrate that different lipid binding proteins vary in their effects on tissue factor activity.",
keywords = "Tissue factor, apolipoproteins, inhibitors",
author = "Carson, {Steven D.} and Ross, {Sherman E.}",
year = "1988",
month = "6",
day = "1",
doi = "10.1016/0049-3848(88)90325-8",
language = "English (US)",
volume = "50",
pages = "669--678",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Effects of lipid-binding proteins APO A-I, APO A-II, β2-glycoprotein I, and C-reactive protein on activation of factor X by tissue factor - factor VIIa

AU - Carson, Steven D.

AU - Ross, Sherman E.

PY - 1988/6/1

Y1 - 1988/6/1

N2 - Tissue factor is the membrane-associated protein which mediates activation of factors IX and X by factor VII. In a purified, reconstituted bovine system, factor X activation by the tissue factor-factor VIIa complex is inhibited by the mixed apoproteins from human high density lipoprotein (HDL) and by isolated apolipoprotein A-II (apo A-II). Other proteins found associated with plasma lipoproteins, apolipoprotein A-I (apo A-I), C-reactive protein (CRP), and β2-glycoprotein I (β2GPI), have been examined for effects on the activation of factor X by tissue factor-factor VIIa. In these experiments, bovine tissue factor, reconstituted into phosphatidylserine-phosphatidylcholine (PS/PC; 30 70) vesicles, was used at a single concentration while factor X (the substrate), factor VIIa (the enzyme), and the potentially inhibitory proteins were varied in a continuous chromogenic assay. Apo A-II and CRP clearly inhibit tissue factor-factor VIIa activation of factor X, while apo A-I and β2GPI have little or no effect. These results demonstrate that different lipid binding proteins vary in their effects on tissue factor activity.

AB - Tissue factor is the membrane-associated protein which mediates activation of factors IX and X by factor VII. In a purified, reconstituted bovine system, factor X activation by the tissue factor-factor VIIa complex is inhibited by the mixed apoproteins from human high density lipoprotein (HDL) and by isolated apolipoprotein A-II (apo A-II). Other proteins found associated with plasma lipoproteins, apolipoprotein A-I (apo A-I), C-reactive protein (CRP), and β2-glycoprotein I (β2GPI), have been examined for effects on the activation of factor X by tissue factor-factor VIIa. In these experiments, bovine tissue factor, reconstituted into phosphatidylserine-phosphatidylcholine (PS/PC; 30 70) vesicles, was used at a single concentration while factor X (the substrate), factor VIIa (the enzyme), and the potentially inhibitory proteins were varied in a continuous chromogenic assay. Apo A-II and CRP clearly inhibit tissue factor-factor VIIa activation of factor X, while apo A-I and β2GPI have little or no effect. These results demonstrate that different lipid binding proteins vary in their effects on tissue factor activity.

KW - Tissue factor

KW - apolipoproteins

KW - inhibitors

UR - http://www.scopus.com/inward/record.url?scp=0023924115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023924115&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(88)90325-8

DO - 10.1016/0049-3848(88)90325-8

M3 - Article

C2 - 3137684

AN - SCOPUS:0023924115

VL - 50

SP - 669

EP - 678

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 5

ER -