Effects of intravitreal indocyanine green injection in rabbits

Mauricio Maia, Eyal Margalit, Rohit Lakhanpal, Mark O.M. Tso, Rhonda Grebe, Gustavo Torres, Kah Guan Au Eong, Michel E. Farah, Gildo Y. Fujii, James Weiland, Eugene De Juan, Salvadori A. D'Anna, Mark S. Humayun

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Purpose: To report the clinical, electrophysiologic, and histologic findings of different concentrations of indocyanine green (ICG) injected into the vitreous cavity of rabbit eyes. Methods: Forty-two rabbits underwent intravitreal injection of 0.1 mL of ICG in three different concentrations: 0.5 mg/mL (250 mOsm), 5 mg/mL (270 mOsm), and 25 mg/mL (170 mOsm). Fellow eyes were injected with 0.1 mL of balanced salt solution. Biomicroscopy, ophthalmoscopy, electroretinography, fluorescein angiography, and histologic evaluation were performed. Results: Eyes injected with 0.5 mg/mL of ICG showed b-wave latency delay on the first day after injection. Eyes injected with 5 mg/mL of ICG showed b-wave latency delay and decreased b-wave amplitude on the first and seventh days after injection; delayed a-wave latency on the first day after injection was also observed. Eyes injected with 25 mg/mL of ICG showed b- and a-wave amplitude and latency abnormalities during the entire followup. Direct correlation of increasing retinal edema proportional to the progressively increasing ICG concentrations was shown on histologic evaluation. Conclusion: Intravitreal ICG injection in rabbit eyes may impair retinal function and morphology proportional to the progressively increasing ICG dosages.

Original languageEnglish (US)
Pages (from-to)69-79
Number of pages11
JournalRetina
Volume24
Issue number1
DOIs
Publication statusPublished - Feb 1 2004

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Keywords

  • Electroretinography
  • Indocyanine green
  • Internal limiting membrane
  • Intraocular toxicity
  • Retina
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Maia, M., Margalit, E., Lakhanpal, R., Tso, M. O. M., Grebe, R., Torres, G., ... Humayun, M. S. (2004). Effects of intravitreal indocyanine green injection in rabbits. Retina, 24(1), 69-79. https://doi.org/10.1097/00006982-200402000-00011