Effects of human chorionic gonadotropin, prostaglandin F and protein kinase C activators on the cyclic AMP and inositol phosphate second messenger systems in cultured human granulosa-luteal cells

John S. Davis, Thomas A. Tedesco, Leigh A. West, George B. Maroulis, Laura L. Weakland

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effects of human chorionic gonadotropin (hCG) and prostaglandin F (PGF) on the adenylate cyclase-cAMP and inositol phospholipid-phospholipase C-inositol trisphosphate and diacylglycerol transmembrane signalling systems were evaluated in cultured human granulosa-luteal cells. Granulosa-luteal cells obtained from patients undergoing in vitro fertilization were cultured for 72 h prior to addition of hormones. During the last 24 h of culture granulosa-luteal cells were incubated with [3H]inositol. Neither hCG nor gonadotropin-releasing hormone (GnRH) stimulated the nositol pholipid-phospholipase C signalling system. PGF stimulated increases in inositol mono-, bis-, and trisphosphate accumulation in 30 min incubations. NaF (20 mM) mimicked the stimulatory effect of PGF on inositol phosphate accumulation suggesting the involvement of a guanine nucleotide regulatory protein in the activation of phospholipase C. In contrast, hCG but not PGF or NaF stimulated cAMP accumulation in 30 min incubations. Simultaneous treatment with hCG and PGF did not alter the stimulatory effect of PGF on inositol phosphate accumulation but reduced (37%) the stimulatory effect of hCG on cAMP accumulation. The protein kinase C activator, 12-O-tetradecanoylphorbol 13-acetate (TPA) inhibited the stimulatory effects of hCG (76%) and PGF (62%) on cAMP and inositol phosphate accumulation, respectively. Thus, cultures of human granulosa-luteal cells possess multiple transmembrane signalling systems which may be modulated by the activation of protein kinase C.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume65
Issue number1-2
DOIs
StatePublished - Aug 1989

Fingerprint

Luteal Cells
Inositol Phosphates
Dinoprost
Second Messenger Systems
Chorionic Gonadotropin
Cyclic AMP
Protein Kinase C
Type C Phospholipases
Inositol
Chemical activation
Diglycerides
Tetradecanoylphorbol Acetate
Fertilization in Vitro
Phosphatidylinositols
GTP-Binding Proteins
Adenylyl Cyclases
Gonadotropin-Releasing Hormone
Acetates
Hormones

Keywords

  • Adenylate cyclase
  • Calcium
  • Hormone action
  • Inositol trisphosphate
  • Ovary
  • Phospholipase C
  • Protein kinase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Effects of human chorionic gonadotropin, prostaglandin F and protein kinase C activators on the cyclic AMP and inositol phosphate second messenger systems in cultured human granulosa-luteal cells. / Davis, John S.; Tedesco, Thomas A.; West, Leigh A.; Maroulis, George B.; Weakland, Laura L.

In: Molecular and Cellular Endocrinology, Vol. 65, No. 1-2, 08.1989, p. 187-193.

Research output: Contribution to journalArticle

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abstract = "The effects of human chorionic gonadotropin (hCG) and prostaglandin F2α (PGF2α) on the adenylate cyclase-cAMP and inositol phospholipid-phospholipase C-inositol trisphosphate and diacylglycerol transmembrane signalling systems were evaluated in cultured human granulosa-luteal cells. Granulosa-luteal cells obtained from patients undergoing in vitro fertilization were cultured for 72 h prior to addition of hormones. During the last 24 h of culture granulosa-luteal cells were incubated with [3H]inositol. Neither hCG nor gonadotropin-releasing hormone (GnRH) stimulated the nositol pholipid-phospholipase C signalling system. PGF2α stimulated increases in inositol mono-, bis-, and trisphosphate accumulation in 30 min incubations. NaF (20 mM) mimicked the stimulatory effect of PGF2α on inositol phosphate accumulation suggesting the involvement of a guanine nucleotide regulatory protein in the activation of phospholipase C. In contrast, hCG but not PGF2α or NaF stimulated cAMP accumulation in 30 min incubations. Simultaneous treatment with hCG and PGF2α did not alter the stimulatory effect of PGF2α on inositol phosphate accumulation but reduced (37{\%}) the stimulatory effect of hCG on cAMP accumulation. The protein kinase C activator, 12-O-tetradecanoylphorbol 13-acetate (TPA) inhibited the stimulatory effects of hCG (76{\%}) and PGF2α (62{\%}) on cAMP and inositol phosphate accumulation, respectively. Thus, cultures of human granulosa-luteal cells possess multiple transmembrane signalling systems which may be modulated by the activation of protein kinase C.",
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KW - Hormone action

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