Effects of high-pressure processing and enzymatic dephosphorylation on phosvitin properties

Stephanie P. Volk, Dong U. Ahn, Michael Zeece, Stephanie Jung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Egg phosvitin could be a good source of functional peptides. Enzymatic dephosphorylation and high-pressure processing combined with thermal treatment applied before proteolysis could produce phosvitin hydrolysates with different properties compared to its native form. Results: Phosvitin structure was maintained overall during high-pressure treatment of 600 MPa applied at an initial temperature of 65 °C regardless of the pH and duration of treatment, confirming the high structural stability of this phosphoprotein. Treatment of phosvitin with phosphatase increased the degree of dephosphorylation from 24% to 63%, after 2 and 18 h, respectively. Moderate dephosphorylation of phosvitin prior to proteolytic digestion improved its hydrolysis, allowing formation of peptides with a molecular weight lower than 17,000 kDa as determined by size exclusion chromatography. Angiotensin-converting enzyme (ACE) inhibition and antioxidant activity of dephosphorylated and protease-treated phosvitin was increased by 52% and 39%, respectively, as compared to protease-digested native phosvitin. Conclusion: Enzymatic dephosphorylation before proteolysis mimicking in vivo gut conditions improved ACE inhibition and antioxidant activity of phosvitin hydrolysates.

Original languageEnglish (US)
Pages (from-to)3095-3098
Number of pages4
JournalJournal of the Science of Food and Agriculture
Volume92
Issue number15
DOIs
StatePublished - Dec 1 2012

Fingerprint

Phosvitin
dephosphorylation
high pressure treatment
Pressure
enzyme inhibition
peptidyl-dipeptidase A
hydrolysates
proteolysis
proteinases
antioxidant activity
peptides
phosphoproteins
Peptidyl-Dipeptidase A
Proteolysis
Peptide Hydrolases
digestive system
Antioxidants
hydrolysis
digestion
heat treatment

Keywords

  • Enzymatic dephosphorylation
  • High-pressure processing
  • Phosvitin

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Cite this

Effects of high-pressure processing and enzymatic dephosphorylation on phosvitin properties. / Volk, Stephanie P.; Ahn, Dong U.; Zeece, Michael; Jung, Stephanie.

In: Journal of the Science of Food and Agriculture, Vol. 92, No. 15, 01.12.2012, p. 3095-3098.

Research output: Contribution to journalArticle

Volk, Stephanie P. ; Ahn, Dong U. ; Zeece, Michael ; Jung, Stephanie. / Effects of high-pressure processing and enzymatic dephosphorylation on phosvitin properties. In: Journal of the Science of Food and Agriculture. 2012 ; Vol. 92, No. 15. pp. 3095-3098.
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