Effects of exercise in a cold environment on gene expression for mitochondrial biogenesis and mitophagy

Megan Opichka, Robert Shute, Katherine Marshall, Dustin R Slivka

Research output: Contribution to journalArticle

Abstract

Cold exposure during cycling and recovery enhances PGC-1α transcription, but aspects of mitophagy and a more intense cold exposure without recovery occurring in the cold have not been explored. Purpose: Determine the expression of genes related to mitochondrial biogenesis and mitophagy following an acute cycling bout at a temperature below freezing compared to that of room temperature. Methods: Eleven male participants cycled at 65% Wmax for 1 h at −2 °C and 20 °C and then recovered at room temperature for 6 h. A muscle biopsy was taken from the vastus lateralis before exercise, 3 h, and 6 h post-exercise for gene expression analysis. Results: Exercising heart rate and skin temperature were lower in the cold (p < 0.001; p = 0.004), while core temperature was higher (p = 0.016). Temperature had no effect on gene expression (p > 0.05). BNIP3 and BNIP3L mRNA were not influenced by exercise (p = 0.329; p 0.233). PGC-1α and VEGF were higher after cycling (p < 0.001), but the extent of PGC-1α upregulation was reduced 6 h post-exercise (p 0.006). TFAM increased 6 h post-exercise (p = 0.001). NRF2, ERRα, PINK1, and PARK2 decreased 3 h post-exercise (p 0.035; p = 0.005; p = 0.002; p = 0.001), but this downregulation was diminished after 6 h of recovery (p = 0.017; p 0.006; p = 0.043; p = 0.047). NRF1 was marginally attenuated with exercise (p = 0.001). Conclusions: Exercise induced alterations in gene expression for mitochondrial biogenesis and mitophagy, but these effects were independent of temperature.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalCryobiology
Volume90
DOIs
StatePublished - Oct 1 2019

Fingerprint

Mitochondrial Degradation
Organelle Biogenesis
Gene expression
exercise
Gene Expression
gene expression
Temperature
Recovery
Skin Temperature
Quadriceps Muscle
Biopsy
Vascular Endothelial Growth Factor A
Freezing
Transcription
ambient temperature
Up-Regulation
Down-Regulation
Heart Rate
Muscle
Skin

Keywords

  • Cycling
  • Human
  • PGC-1α
  • Skeletal muscle
  • Temperature
  • mRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Effects of exercise in a cold environment on gene expression for mitochondrial biogenesis and mitophagy. / Opichka, Megan; Shute, Robert; Marshall, Katherine; Slivka, Dustin R.

In: Cryobiology, Vol. 90, 01.10.2019, p. 47-53.

Research output: Contribution to journalArticle

Opichka, Megan ; Shute, Robert ; Marshall, Katherine ; Slivka, Dustin R. / Effects of exercise in a cold environment on gene expression for mitochondrial biogenesis and mitophagy. In: Cryobiology. 2019 ; Vol. 90. pp. 47-53.
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abstract = "Cold exposure during cycling and recovery enhances PGC-1α transcription, but aspects of mitophagy and a more intense cold exposure without recovery occurring in the cold have not been explored. Purpose: Determine the expression of genes related to mitochondrial biogenesis and mitophagy following an acute cycling bout at a temperature below freezing compared to that of room temperature. Methods: Eleven male participants cycled at 65{\%} Wmax for 1 h at −2 °C and 20 °C and then recovered at room temperature for 6 h. A muscle biopsy was taken from the vastus lateralis before exercise, 3 h, and 6 h post-exercise for gene expression analysis. Results: Exercising heart rate and skin temperature were lower in the cold (p < 0.001; p = 0.004), while core temperature was higher (p = 0.016). Temperature had no effect on gene expression (p > 0.05). BNIP3 and BNIP3L mRNA were not influenced by exercise (p = 0.329; p 0.233). PGC-1α and VEGF were higher after cycling (p < 0.001), but the extent of PGC-1α upregulation was reduced 6 h post-exercise (p 0.006). TFAM increased 6 h post-exercise (p = 0.001). NRF2, ERRα, PINK1, and PARK2 decreased 3 h post-exercise (p 0.035; p = 0.005; p = 0.002; p = 0.001), but this downregulation was diminished after 6 h of recovery (p = 0.017; p 0.006; p = 0.043; p = 0.047). NRF1 was marginally attenuated with exercise (p = 0.001). Conclusions: Exercise induced alterations in gene expression for mitochondrial biogenesis and mitophagy, but these effects were independent of temperature.",
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AU - Opichka, Megan

AU - Shute, Robert

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AU - Slivka, Dustin R

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N2 - Cold exposure during cycling and recovery enhances PGC-1α transcription, but aspects of mitophagy and a more intense cold exposure without recovery occurring in the cold have not been explored. Purpose: Determine the expression of genes related to mitochondrial biogenesis and mitophagy following an acute cycling bout at a temperature below freezing compared to that of room temperature. Methods: Eleven male participants cycled at 65% Wmax for 1 h at −2 °C and 20 °C and then recovered at room temperature for 6 h. A muscle biopsy was taken from the vastus lateralis before exercise, 3 h, and 6 h post-exercise for gene expression analysis. Results: Exercising heart rate and skin temperature were lower in the cold (p < 0.001; p = 0.004), while core temperature was higher (p = 0.016). Temperature had no effect on gene expression (p > 0.05). BNIP3 and BNIP3L mRNA were not influenced by exercise (p = 0.329; p 0.233). PGC-1α and VEGF were higher after cycling (p < 0.001), but the extent of PGC-1α upregulation was reduced 6 h post-exercise (p 0.006). TFAM increased 6 h post-exercise (p = 0.001). NRF2, ERRα, PINK1, and PARK2 decreased 3 h post-exercise (p 0.035; p = 0.005; p = 0.002; p = 0.001), but this downregulation was diminished after 6 h of recovery (p = 0.017; p 0.006; p = 0.043; p = 0.047). NRF1 was marginally attenuated with exercise (p = 0.001). Conclusions: Exercise induced alterations in gene expression for mitochondrial biogenesis and mitophagy, but these effects were independent of temperature.

AB - Cold exposure during cycling and recovery enhances PGC-1α transcription, but aspects of mitophagy and a more intense cold exposure without recovery occurring in the cold have not been explored. Purpose: Determine the expression of genes related to mitochondrial biogenesis and mitophagy following an acute cycling bout at a temperature below freezing compared to that of room temperature. Methods: Eleven male participants cycled at 65% Wmax for 1 h at −2 °C and 20 °C and then recovered at room temperature for 6 h. A muscle biopsy was taken from the vastus lateralis before exercise, 3 h, and 6 h post-exercise for gene expression analysis. Results: Exercising heart rate and skin temperature were lower in the cold (p < 0.001; p = 0.004), while core temperature was higher (p = 0.016). Temperature had no effect on gene expression (p > 0.05). BNIP3 and BNIP3L mRNA were not influenced by exercise (p = 0.329; p 0.233). PGC-1α and VEGF were higher after cycling (p < 0.001), but the extent of PGC-1α upregulation was reduced 6 h post-exercise (p 0.006). TFAM increased 6 h post-exercise (p = 0.001). NRF2, ERRα, PINK1, and PARK2 decreased 3 h post-exercise (p 0.035; p = 0.005; p = 0.002; p = 0.001), but this downregulation was diminished after 6 h of recovery (p = 0.017; p 0.006; p = 0.043; p = 0.047). NRF1 was marginally attenuated with exercise (p = 0.001). Conclusions: Exercise induced alterations in gene expression for mitochondrial biogenesis and mitophagy, but these effects were independent of temperature.

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