Effects of early and late chronic pressure overload on extracellular matrix remodeling

Jing Lin, Harrison B. Davis, Quixia Dai, Youn Min Chou, Teresa Craig, Carmen Hinojosa-Laborde, Merry L. Lindsey

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The left ventricle (LV) remodels with age and in response to pressure overload. While aging and pressure overload are superimposed in the clinical context, the structural and functional consequences of the individual processes are not well-understood. Accordingly, the objective of this study was to compare the effects of both early and late chronic hypertension on extracellular matrix (ECM) remodeling. The following groups of Dahl rats were studied: 1) young salt-resistant (control, n=6); 2) young salt-sensitive (early phase of chronic hypertension, n=6) 3) middle-aged salt-resistant (aging, n=5); and 4) middle-aged salt-sensitive (late phase of chronic hypertension, n=6). We measured LV mass (LVM) and body weight (BW) and Immunoblotted a panel of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and ECM proteins. Total collagen Increased, several MMP-8 decreased and TIMP-1 increased in the early phase of hypertension, consistent with fibrosis. Active MMP-8 decreased from 8,010±81 U in young salt-resistant to 5,260±313 U in young salt-sensitive (p<0.05) rats. During the late phase, chronic hypertension decreased total collagen levels and increased MMP-8 and MMP-14 (all p<0.05). Based on good-fit modeling analysis, MMP-14 (45 kDa) correlated positively with changes in LVM/BW during the early phase. In conclusion, this is the first study to evaluate MMP levels during both early and late chronic phases of hypertension. Our results highlight that ECM remodeling in response to pressure overload is a dynamic process involving excessive ECM accumulation and degradation.

Original languageEnglish (US)
Pages (from-to)1225-1231
Number of pages7
JournalHypertension Research
Volume31
Issue number6
DOIs
StatePublished - Jun 1 2008

Fingerprint

Extracellular Matrix
Salts
Matrix Metalloproteinase 8
Hypertension
Pressure
Matrix Metalloproteinase 14
Heart Ventricles
Collagen
Body Weight
Inbred Dahl Rats
Tissue Inhibitor of Metalloproteinases
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Extracellular Matrix Proteins
Matrix Metalloproteinases
Fibrosis

Keywords

  • Aging
  • Hypertension
  • Hypertrophy
  • Matrix metalloproteinases
  • Tissue inhibitor of metalloproteinase

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Effects of early and late chronic pressure overload on extracellular matrix remodeling. / Lin, Jing; Davis, Harrison B.; Dai, Quixia; Chou, Youn Min; Craig, Teresa; Hinojosa-Laborde, Carmen; Lindsey, Merry L.

In: Hypertension Research, Vol. 31, No. 6, 01.06.2008, p. 1225-1231.

Research output: Contribution to journalArticle

Lin, J, Davis, HB, Dai, Q, Chou, YM, Craig, T, Hinojosa-Laborde, C & Lindsey, ML 2008, 'Effects of early and late chronic pressure overload on extracellular matrix remodeling', Hypertension Research, vol. 31, no. 6, pp. 1225-1231. https://doi.org/10.1291/hypres.31.1225
Lin J, Davis HB, Dai Q, Chou YM, Craig T, Hinojosa-Laborde C et al. Effects of early and late chronic pressure overload on extracellular matrix remodeling. Hypertension Research. 2008 Jun 1;31(6):1225-1231. https://doi.org/10.1291/hypres.31.1225
Lin, Jing ; Davis, Harrison B. ; Dai, Quixia ; Chou, Youn Min ; Craig, Teresa ; Hinojosa-Laborde, Carmen ; Lindsey, Merry L. / Effects of early and late chronic pressure overload on extracellular matrix remodeling. In: Hypertension Research. 2008 ; Vol. 31, No. 6. pp. 1225-1231.
@article{304c17a8c14c43cc8250c439bae8889e,
title = "Effects of early and late chronic pressure overload on extracellular matrix remodeling",
abstract = "The left ventricle (LV) remodels with age and in response to pressure overload. While aging and pressure overload are superimposed in the clinical context, the structural and functional consequences of the individual processes are not well-understood. Accordingly, the objective of this study was to compare the effects of both early and late chronic hypertension on extracellular matrix (ECM) remodeling. The following groups of Dahl rats were studied: 1) young salt-resistant (control, n=6); 2) young salt-sensitive (early phase of chronic hypertension, n=6) 3) middle-aged salt-resistant (aging, n=5); and 4) middle-aged salt-sensitive (late phase of chronic hypertension, n=6). We measured LV mass (LVM) and body weight (BW) and Immunoblotted a panel of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and ECM proteins. Total collagen Increased, several MMP-8 decreased and TIMP-1 increased in the early phase of hypertension, consistent with fibrosis. Active MMP-8 decreased from 8,010±81 U in young salt-resistant to 5,260±313 U in young salt-sensitive (p<0.05) rats. During the late phase, chronic hypertension decreased total collagen levels and increased MMP-8 and MMP-14 (all p<0.05). Based on good-fit modeling analysis, MMP-14 (45 kDa) correlated positively with changes in LVM/BW during the early phase. In conclusion, this is the first study to evaluate MMP levels during both early and late chronic phases of hypertension. Our results highlight that ECM remodeling in response to pressure overload is a dynamic process involving excessive ECM accumulation and degradation.",
keywords = "Aging, Hypertension, Hypertrophy, Matrix metalloproteinases, Tissue inhibitor of metalloproteinase",
author = "Jing Lin and Davis, {Harrison B.} and Quixia Dai and Chou, {Youn Min} and Teresa Craig and Carmen Hinojosa-Laborde and Lindsey, {Merry L.}",
year = "2008",
month = "6",
day = "1",
doi = "10.1291/hypres.31.1225",
language = "English (US)",
volume = "31",
pages = "1225--1231",
journal = "Hypertension Research",
issn = "0916-9636",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Effects of early and late chronic pressure overload on extracellular matrix remodeling

AU - Lin, Jing

AU - Davis, Harrison B.

AU - Dai, Quixia

AU - Chou, Youn Min

AU - Craig, Teresa

AU - Hinojosa-Laborde, Carmen

AU - Lindsey, Merry L.

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The left ventricle (LV) remodels with age and in response to pressure overload. While aging and pressure overload are superimposed in the clinical context, the structural and functional consequences of the individual processes are not well-understood. Accordingly, the objective of this study was to compare the effects of both early and late chronic hypertension on extracellular matrix (ECM) remodeling. The following groups of Dahl rats were studied: 1) young salt-resistant (control, n=6); 2) young salt-sensitive (early phase of chronic hypertension, n=6) 3) middle-aged salt-resistant (aging, n=5); and 4) middle-aged salt-sensitive (late phase of chronic hypertension, n=6). We measured LV mass (LVM) and body weight (BW) and Immunoblotted a panel of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and ECM proteins. Total collagen Increased, several MMP-8 decreased and TIMP-1 increased in the early phase of hypertension, consistent with fibrosis. Active MMP-8 decreased from 8,010±81 U in young salt-resistant to 5,260±313 U in young salt-sensitive (p<0.05) rats. During the late phase, chronic hypertension decreased total collagen levels and increased MMP-8 and MMP-14 (all p<0.05). Based on good-fit modeling analysis, MMP-14 (45 kDa) correlated positively with changes in LVM/BW during the early phase. In conclusion, this is the first study to evaluate MMP levels during both early and late chronic phases of hypertension. Our results highlight that ECM remodeling in response to pressure overload is a dynamic process involving excessive ECM accumulation and degradation.

AB - The left ventricle (LV) remodels with age and in response to pressure overload. While aging and pressure overload are superimposed in the clinical context, the structural and functional consequences of the individual processes are not well-understood. Accordingly, the objective of this study was to compare the effects of both early and late chronic hypertension on extracellular matrix (ECM) remodeling. The following groups of Dahl rats were studied: 1) young salt-resistant (control, n=6); 2) young salt-sensitive (early phase of chronic hypertension, n=6) 3) middle-aged salt-resistant (aging, n=5); and 4) middle-aged salt-sensitive (late phase of chronic hypertension, n=6). We measured LV mass (LVM) and body weight (BW) and Immunoblotted a panel of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and ECM proteins. Total collagen Increased, several MMP-8 decreased and TIMP-1 increased in the early phase of hypertension, consistent with fibrosis. Active MMP-8 decreased from 8,010±81 U in young salt-resistant to 5,260±313 U in young salt-sensitive (p<0.05) rats. During the late phase, chronic hypertension decreased total collagen levels and increased MMP-8 and MMP-14 (all p<0.05). Based on good-fit modeling analysis, MMP-14 (45 kDa) correlated positively with changes in LVM/BW during the early phase. In conclusion, this is the first study to evaluate MMP levels during both early and late chronic phases of hypertension. Our results highlight that ECM remodeling in response to pressure overload is a dynamic process involving excessive ECM accumulation and degradation.

KW - Aging

KW - Hypertension

KW - Hypertrophy

KW - Matrix metalloproteinases

KW - Tissue inhibitor of metalloproteinase

UR - http://www.scopus.com/inward/record.url?scp=48349145875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48349145875&partnerID=8YFLogxK

U2 - 10.1291/hypres.31.1225

DO - 10.1291/hypres.31.1225

M3 - Article

C2 - 18716372

AN - SCOPUS:48349145875

VL - 31

SP - 1225

EP - 1231

JO - Hypertension Research

JF - Hypertension Research

SN - 0916-9636

IS - 6

ER -