Effects of chitosan on the protein profile of grape cell culture subcellular fractions

Maura Ferri, Marina Franceschetti, Michael J. Naldrett, Gerhard Saalbach, Annalisa Tassoni

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Grapevine is a large source of healthy polyphenols for human diet, and red table-grapes and wines are the main source of stilbenes. These compounds are important both in the plant defence system and for human health. In the present study, Vitis vinifera cv. Barbera cell cultures were treated with 50 μg/mL chitosan and proteomic analyses on soluble and membrane subcellular fractions were performed against suitable controls. Three soluble stilbene synthase protein spots, four stilbene synthase spots in the microsomal fraction and four spots of membrane ATPase subunits were identified, the accumulation of which was modulated in response to chitosan treatment. Present proteomic and immunolocalisation data seem to provide evidence supporting the hypothesis that a stilbene biosynthetic multi-enzyme complex is associated with the intracellular membrane. In addition, proteomic analyses showed a general decrease in the accumulation of proteins belonging to different primary metabolism pathways, both in the soluble and membrane fractions. In particular, energy, sugar and amino acid metabolisms were down-regulated as a consequence of chitosan and acetic acid treatments. These metabolic modifications could lead to a consistent change in the profile and amount of metabolites stored in grape berries, with consequent effects on taste, flavour, organoleptic and nutraceutical properties of derived food products.

Original languageEnglish (US)
Pages (from-to)1685-1692
Number of pages8
JournalELECTROPHORESIS
Volume35
Issue number11
DOIs
StatePublished - Jun 2014

Fingerprint

Subcellular Fractions
Vitis
Chitosan
Cell culture
Proteomics
Stilbenes
Cell Culture Techniques
Membranes
Metabolism
Sugar Acids
Intracellular Membranes
Proteins
Polyphenols
Wine
Dietary Supplements
Acetic Acid
Adenosine Triphosphatases
Flavors
Fruit
Nutrition

Keywords

  • Chitosan
  • In vitro cell cultures
  • Stilbene synthase
  • Vitis vinifera (grape)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Effects of chitosan on the protein profile of grape cell culture subcellular fractions. / Ferri, Maura; Franceschetti, Marina; Naldrett, Michael J.; Saalbach, Gerhard; Tassoni, Annalisa.

In: ELECTROPHORESIS, Vol. 35, No. 11, 06.2014, p. 1685-1692.

Research output: Contribution to journalArticle

Ferri, Maura ; Franceschetti, Marina ; Naldrett, Michael J. ; Saalbach, Gerhard ; Tassoni, Annalisa. / Effects of chitosan on the protein profile of grape cell culture subcellular fractions. In: ELECTROPHORESIS. 2014 ; Vol. 35, No. 11. pp. 1685-1692.
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