Effects of botulinum neurotoxin and Lambert-Eaton myasthenic syndrome IgG at mouse nerve terminals

S. Lande, J. Black, J. O. Dolly, B. Lang, J. Newsom-Davis, D. W. -Wray

Research output: Contribution to journalArticle

Abstract

The interaction between two presynaptically acting agents, Lambert-Eaton myasthenic syndrome (LEMS) immunoglobulin G (IgG) and purified botulinum neurotoxin (BoNT) type A, was studied. Intracellular microelectrode recordings were carried out on mouse muscles after injection with LEMS IgG. BoNT was either injected before recordings were made or applied in vitro. The time course of the in vitro actions of BoNT on miniature end-plate potential and end-plate potential parameters were not affected by pretreatment with LEMS IgG. After in vivo injection of BoNT, end-plate potential quantal content was reduced to less than 2% of control values, whether or not LEMS IgG had also been previously given. Quantitative electron-microscope autoradiographical analysis showed that neither the binding of125I-BoNT to acceptors on the nerve terminal membrane nor the pattern of its internalisation were affected by pretreatment with LEMS IgG. We conclude that the effects of BoNT are not affected by LEMS IgG, suggesting different presynaptic binding sites for the two agents.

Original languageEnglish (US)
Pages (from-to)229-242
Number of pages14
JournalJournal of Neural Transmission - Parkinson's Disease and Dementia Section
Volume1
Issue number3
DOIs
StatePublished - Sep 1 1989

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Lambert-Eaton Myasthenic Syndrome
Neurotoxins
Immunoglobulin G
Excitatory Postsynaptic Potentials
Miniature Postsynaptic Potentials
Type A Botulinum Toxins
Injections
Microelectrodes
Binding Sites
Electrons
Muscles
Membranes

Keywords

  • Botulinum toxin
  • antibody
  • neuromuscular junction
  • transmitter release

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

Cite this

Effects of botulinum neurotoxin and Lambert-Eaton myasthenic syndrome IgG at mouse nerve terminals. / Lande, S.; Black, J.; Dolly, J. O.; Lang, B.; Newsom-Davis, J.; -Wray, D. W.

In: Journal of Neural Transmission - Parkinson's Disease and Dementia Section, Vol. 1, No. 3, 01.09.1989, p. 229-242.

Research output: Contribution to journalArticle

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AU - Newsom-Davis, J.

AU - -Wray, D. W.

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