Effects of 8-phenyltheophylline and glibenclamide on cardiovascular responses to adenosine and R-N6 (phenylisopropyl)-adenosine in anesthetized rats

Yulong Li, R. R. He, S. Y. Ho

Research output: Contribution to journalArticle

Abstract

The hemodynamic effects of R-N6-(phenylisopropyl)-adenosine (R-PIA, a A1 selective adenosine receptor agonist) and the antagonistic effects of 8-phenyltheophylline (a nonselective adenosine receptor antagonist) and glibenclamide (ATP sensitive potassium channel antagonist) were investigated in 72 anesthetized Sprague-Dawley rats. The results were as follows: 1) Following the injection of R-PIA (0.001-0.05 μmol/kg), MAP, HR and CI were remarkably decreased without initial pressor response but TPRI was slightly decreased only in high dose of R-PIA; 2) 8-phenyltheophylline (0.05-0.2 μmol/kg) could inhibit the effects of R-PIA; 3) Glibenclamide (5 and 10 mg/kg) could attenuate the initial pressor and delayed depressor effects with bradycardia induced by adenosine (1 μmol/kg). From these results obtained, it is hypothesized that adenosine receptors in carotid body are A2-receptor subtype mediating the pressor effect of adenosine and its analogue, the negative chronotropic or/and inotropic effects of adenosine and its analogue are involved in their hypotensive action, and ATP-sensitive K+ channels coupled by adenosine receptors may be activated by adenosine to mediate its effects.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalChinese Journal of Physiological Sciences
Volume10
Issue number2
StatePublished - Jan 1 1994

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Glyburide
Adenosine
Purinergic P1 Receptors
Adenosine A1 Receptor Agonists
Purinergic P1 Receptor Antagonists
Carotid Body
KATP Channels
Bradycardia
varespladib methyl
Sprague Dawley Rats
Adenosine Triphosphate
Hemodynamics
8-phenyltheophylline
Injections
N-(1-methyl-2-phenylethyl)adenosine

Keywords

  • 8-phenyltheo phylline
  • ATP-sensitive K channel
  • R-N-(phenylisopropyl)-adenosine
  • adenosine
  • adenosine receptor
  • glibenclamide
  • hemodynamics

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Effects of 8-phenyltheophylline and glibenclamide on cardiovascular responses to adenosine and R-N6 (phenylisopropyl)-adenosine in anesthetized rats",
abstract = "The hemodynamic effects of R-N6-(phenylisopropyl)-adenosine (R-PIA, a A1 selective adenosine receptor agonist) and the antagonistic effects of 8-phenyltheophylline (a nonselective adenosine receptor antagonist) and glibenclamide (ATP sensitive potassium channel antagonist) were investigated in 72 anesthetized Sprague-Dawley rats. The results were as follows: 1) Following the injection of R-PIA (0.001-0.05 μmol/kg), MAP, HR and CI were remarkably decreased without initial pressor response but TPRI was slightly decreased only in high dose of R-PIA; 2) 8-phenyltheophylline (0.05-0.2 μmol/kg) could inhibit the effects of R-PIA; 3) Glibenclamide (5 and 10 mg/kg) could attenuate the initial pressor and delayed depressor effects with bradycardia induced by adenosine (1 μmol/kg). From these results obtained, it is hypothesized that adenosine receptors in carotid body are A2-receptor subtype mediating the pressor effect of adenosine and its analogue, the negative chronotropic or/and inotropic effects of adenosine and its analogue are involved in their hypotensive action, and ATP-sensitive K+ channels coupled by adenosine receptors may be activated by adenosine to mediate its effects.",
keywords = "8-phenyltheo phylline, ATP-sensitive K channel, R-N-(phenylisopropyl)-adenosine, adenosine, adenosine receptor, glibenclamide, hemodynamics",
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T1 - Effects of 8-phenyltheophylline and glibenclamide on cardiovascular responses to adenosine and R-N6 (phenylisopropyl)-adenosine in anesthetized rats

AU - Li, Yulong

AU - He, R. R.

AU - Ho, S. Y.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The hemodynamic effects of R-N6-(phenylisopropyl)-adenosine (R-PIA, a A1 selective adenosine receptor agonist) and the antagonistic effects of 8-phenyltheophylline (a nonselective adenosine receptor antagonist) and glibenclamide (ATP sensitive potassium channel antagonist) were investigated in 72 anesthetized Sprague-Dawley rats. The results were as follows: 1) Following the injection of R-PIA (0.001-0.05 μmol/kg), MAP, HR and CI were remarkably decreased without initial pressor response but TPRI was slightly decreased only in high dose of R-PIA; 2) 8-phenyltheophylline (0.05-0.2 μmol/kg) could inhibit the effects of R-PIA; 3) Glibenclamide (5 and 10 mg/kg) could attenuate the initial pressor and delayed depressor effects with bradycardia induced by adenosine (1 μmol/kg). From these results obtained, it is hypothesized that adenosine receptors in carotid body are A2-receptor subtype mediating the pressor effect of adenosine and its analogue, the negative chronotropic or/and inotropic effects of adenosine and its analogue are involved in their hypotensive action, and ATP-sensitive K+ channels coupled by adenosine receptors may be activated by adenosine to mediate its effects.

AB - The hemodynamic effects of R-N6-(phenylisopropyl)-adenosine (R-PIA, a A1 selective adenosine receptor agonist) and the antagonistic effects of 8-phenyltheophylline (a nonselective adenosine receptor antagonist) and glibenclamide (ATP sensitive potassium channel antagonist) were investigated in 72 anesthetized Sprague-Dawley rats. The results were as follows: 1) Following the injection of R-PIA (0.001-0.05 μmol/kg), MAP, HR and CI were remarkably decreased without initial pressor response but TPRI was slightly decreased only in high dose of R-PIA; 2) 8-phenyltheophylline (0.05-0.2 μmol/kg) could inhibit the effects of R-PIA; 3) Glibenclamide (5 and 10 mg/kg) could attenuate the initial pressor and delayed depressor effects with bradycardia induced by adenosine (1 μmol/kg). From these results obtained, it is hypothesized that adenosine receptors in carotid body are A2-receptor subtype mediating the pressor effect of adenosine and its analogue, the negative chronotropic or/and inotropic effects of adenosine and its analogue are involved in their hypotensive action, and ATP-sensitive K+ channels coupled by adenosine receptors may be activated by adenosine to mediate its effects.

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