Effect of surrogate tolerogenesis on the vascular rejection of pig heart xenografts

Debra L. Sudan, Stanley J Radio, Aurelio Matamoros, Noel D. Johnson, C. Carson Shearon, Kimberly R. Glogowski, Arthur C. Hill, Timothy C. Goertzen, Ira J. Fox, Alan Norman Langnas, William E. Beschorner

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Abstract

Background. Organ xenografts are fulminantly rejected by antibody- mediated vascular rejection. Surrogate tolerogenesis (ST), the induction of tolerance within the donor, is effective with aorta xenografts. This preliminary study assesses the effect of ST on preformed antibodies and rejection of porcine heart xenografts. Methods. Tolerance to the donor pig was induced by infusing recipient marrow into fetal pigs. Later, pig splenocytes were transfused and heterotopic pig hearts transplanted using chimeric or nonchimeric pigs. Anti-pig antibodies were assessed. Results. With ST alone, xenografts developed cellular rejection at 4-6 days, whereas control grafts developed vascular rejection at 3-4 days (cellular vs. vascular, P<0.03). There was a reduction in preformed antibodies (P<0.03). ST combined with moderate cyclosporine prevented rejection at 9+ and 25 days in sensitized recipients compared with vascular rejection at 0.5-2 days for controls (P<0.07). Conclusions. ST seems to provide protection against vascular rejection. The cellular rejection seems sensitive to cyclosporine.

Original languageEnglish (US)
Pages (from-to)232-235
Number of pages4
JournalTransplantation
Volume69
Issue number2
DOIs
StatePublished - Jan 27 2000

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Heterografts
Blood Vessels
Swine
Cyclosporine
Antibodies
Aorta
Anti-Idiotypic Antibodies
Bone Marrow
Transplants

ASJC Scopus subject areas

  • Transplantation

Cite this

Sudan, D. L., Radio, S. J., Matamoros, A., Johnson, N. D., Shearon, C. C., Glogowski, K. R., ... Beschorner, W. E. (2000). Effect of surrogate tolerogenesis on the vascular rejection of pig heart xenografts. Transplantation, 69(2), 232-235. https://doi.org/10.1097/00007890-200001270-00007

Effect of surrogate tolerogenesis on the vascular rejection of pig heart xenografts. / Sudan, Debra L.; Radio, Stanley J; Matamoros, Aurelio; Johnson, Noel D.; Shearon, C. Carson; Glogowski, Kimberly R.; Hill, Arthur C.; Goertzen, Timothy C.; Fox, Ira J.; Langnas, Alan Norman; Beschorner, William E.

In: Transplantation, Vol. 69, No. 2, 27.01.2000, p. 232-235.

Research output: Contribution to journalArticle

Sudan, DL, Radio, SJ, Matamoros, A, Johnson, ND, Shearon, CC, Glogowski, KR, Hill, AC, Goertzen, TC, Fox, IJ, Langnas, AN & Beschorner, WE 2000, 'Effect of surrogate tolerogenesis on the vascular rejection of pig heart xenografts', Transplantation, vol. 69, no. 2, pp. 232-235. https://doi.org/10.1097/00007890-200001270-00007
Sudan, Debra L. ; Radio, Stanley J ; Matamoros, Aurelio ; Johnson, Noel D. ; Shearon, C. Carson ; Glogowski, Kimberly R. ; Hill, Arthur C. ; Goertzen, Timothy C. ; Fox, Ira J. ; Langnas, Alan Norman ; Beschorner, William E. / Effect of surrogate tolerogenesis on the vascular rejection of pig heart xenografts. In: Transplantation. 2000 ; Vol. 69, No. 2. pp. 232-235.
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