Effect of sodium saccharin on the neonatal rat bladder

Samuel Monroe Cohen, M. Cano, M. K. John St., E. M. Garland, M. Khachab, L. B. Ellwein, J. B. Reitan, C. Frith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In a two-generation bioassay, high doses of dietary sodium saccharin (NaSac) produce bladder carcinoma in rats, whereas acid saccharin (HSac) does not effect the urothelium. NaSac and HSac administered as 5% of the diet to F0 Sprague-Dawley (SD) and F344 rats, continued through to the weaned male rats for ten additional weeks. Control 3H-thymidine labeling index (LI) was high prior to and at birth (approximately 11%), declining rapidly by weaning (to < 0.2). Neither NaSac nor HSac increased proliferation through 7 days of age. NaSac increased the proliferation rate at later times, whereas HSac did not. The LI decreased to control levels in NaSac-fed rats switched to control diet after weaning and increased in control-fed rats switched to NaSac after birth or weaning. In a second experiment, 5 % NaSac did not affect urothelial morphology of SD rats through 7 days. By 21 days post-birth, urothelial hyperplasia occurred in NaSac-fed rat. The LI in treated versus control was similar through gestation, with a slight difference by 7 days. LI was significantly different by 21 days post-birth, but was similar between males and females. These results provide additional evidence for the increased cell proliferative effects of NaSac during the neonatal period, but not during gestation.

Original languageEnglish (US)
Pages (from-to)137-148
Number of pages12
JournalScanning Microscopy
Volume9
Issue number1
StatePublished - Jan 1 1995

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bladder
rats
Rats
Sodium
sodium
Labeling
marking
diets
Nutrition
thymidine
bioassay
Bioassay
Level control
cancer
dosage
acids
Acids
cells

Keywords

  • Bladder
  • neonatal development
  • proliferation
  • saccharin
  • scanning electron microscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

Cohen, S. M., Cano, M., John St., M. K., Garland, E. M., Khachab, M., Ellwein, L. B., ... Frith, C. (1995). Effect of sodium saccharin on the neonatal rat bladder. Scanning Microscopy, 9(1), 137-148.

Effect of sodium saccharin on the neonatal rat bladder. / Cohen, Samuel Monroe; Cano, M.; John St., M. K.; Garland, E. M.; Khachab, M.; Ellwein, L. B.; Reitan, J. B.; Frith, C.

In: Scanning Microscopy, Vol. 9, No. 1, 01.01.1995, p. 137-148.

Research output: Contribution to journalArticle

Cohen, SM, Cano, M, John St., MK, Garland, EM, Khachab, M, Ellwein, LB, Reitan, JB & Frith, C 1995, 'Effect of sodium saccharin on the neonatal rat bladder', Scanning Microscopy, vol. 9, no. 1, pp. 137-148.
Cohen SM, Cano M, John St. MK, Garland EM, Khachab M, Ellwein LB et al. Effect of sodium saccharin on the neonatal rat bladder. Scanning Microscopy. 1995 Jan 1;9(1):137-148.
Cohen, Samuel Monroe ; Cano, M. ; John St., M. K. ; Garland, E. M. ; Khachab, M. ; Ellwein, L. B. ; Reitan, J. B. ; Frith, C. / Effect of sodium saccharin on the neonatal rat bladder. In: Scanning Microscopy. 1995 ; Vol. 9, No. 1. pp. 137-148.
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