Effect of sodium hydrosulphide after acute compression injury of spinal cord

V. Kesherwani, K. S. Nelson, S. K. Agrawal

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background Early treatment of spinal cord white matter injury has been found beneficial. H2S, a neurotransmitter is neuroprotective at lower doses. Purpose In the present study the effect of NaHS after clip compression injury of spinal cord white matter in vivo was studied. Methods The injury was induced in 8-10 weeks old Wistar rats by exposing the spinal cord at T8-T10 level by laminectomy and applying 35 g clip for 1 min. A dose of 50 μM NaHS was given intraperitoneally after 1 h of injury. 0.5 mm Spinal cord tissues were collected 8 h after injury from both sides including epicenter and dorsal column was microdissected and used for further study. Results NaHS treatment decreases nitric oxide (NO) by 27% and lipid peroxide (LPO) by 18% as compared to injury, which are hallmark of attenuation in oxidative stress. Western blots shows significant changes in Myeloperoxidase (MPO) level went down by 10%. GSH contents increased 44% in treated group as compared to the injury group. NaHS treatment increased Nrf-2 expression 1.8 times. We found NaHS treatment reduced the GFAP expression 8%, there was no significant changes in NF-200 after treatment and no evident morphological changes with H and E staining. Conclusions With the above data we conclude that NaHS at 50 μM dose at 1 h after injury reduces the NO, LPO, GFAP and MPO level at injury site by increasing the expression of Nrf-2. We expect that a decrease in these parameters during acute phase of spinal cord injury would be helpful in neuroprotection and regeneration.

Original languageEnglish (US)
Pages (from-to)222-229
Number of pages8
JournalBrain Research
Volume1527
DOIs
StatePublished - Aug 21 2013

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Spinal Cord Compression
Spinal Cord Injuries
Wounds and Injuries
Spinal Cord
Lipid Peroxides
Surgical Instruments
Peroxidase
Nitric Oxide
Laminectomy
sodium bisulfide
Neurotransmitter Agents
Wistar Rats
Regeneration
Oxidative Stress
Western Blotting
Staining and Labeling

Keywords

  • Hydrogen sulphide
  • Neuroprotection
  • Nrf-2
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Effect of sodium hydrosulphide after acute compression injury of spinal cord. / Kesherwani, V.; Nelson, K. S.; Agrawal, S. K.

In: Brain Research, Vol. 1527, 21.08.2013, p. 222-229.

Research output: Contribution to journalArticle

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abstract = "Background Early treatment of spinal cord white matter injury has been found beneficial. H2S, a neurotransmitter is neuroprotective at lower doses. Purpose In the present study the effect of NaHS after clip compression injury of spinal cord white matter in vivo was studied. Methods The injury was induced in 8-10 weeks old Wistar rats by exposing the spinal cord at T8-T10 level by laminectomy and applying 35 g clip for 1 min. A dose of 50 μM NaHS was given intraperitoneally after 1 h of injury. 0.5 mm Spinal cord tissues were collected 8 h after injury from both sides including epicenter and dorsal column was microdissected and used for further study. Results NaHS treatment decreases nitric oxide (NO) by 27{\%} and lipid peroxide (LPO) by 18{\%} as compared to injury, which are hallmark of attenuation in oxidative stress. Western blots shows significant changes in Myeloperoxidase (MPO) level went down by 10{\%}. GSH contents increased 44{\%} in treated group as compared to the injury group. NaHS treatment increased Nrf-2 expression 1.8 times. We found NaHS treatment reduced the GFAP expression 8{\%}, there was no significant changes in NF-200 after treatment and no evident morphological changes with H and E staining. Conclusions With the above data we conclude that NaHS at 50 μM dose at 1 h after injury reduces the NO, LPO, GFAP and MPO level at injury site by increasing the expression of Nrf-2. We expect that a decrease in these parameters during acute phase of spinal cord injury would be helpful in neuroprotection and regeneration.",
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AB - Background Early treatment of spinal cord white matter injury has been found beneficial. H2S, a neurotransmitter is neuroprotective at lower doses. Purpose In the present study the effect of NaHS after clip compression injury of spinal cord white matter in vivo was studied. Methods The injury was induced in 8-10 weeks old Wistar rats by exposing the spinal cord at T8-T10 level by laminectomy and applying 35 g clip for 1 min. A dose of 50 μM NaHS was given intraperitoneally after 1 h of injury. 0.5 mm Spinal cord tissues were collected 8 h after injury from both sides including epicenter and dorsal column was microdissected and used for further study. Results NaHS treatment decreases nitric oxide (NO) by 27% and lipid peroxide (LPO) by 18% as compared to injury, which are hallmark of attenuation in oxidative stress. Western blots shows significant changes in Myeloperoxidase (MPO) level went down by 10%. GSH contents increased 44% in treated group as compared to the injury group. NaHS treatment increased Nrf-2 expression 1.8 times. We found NaHS treatment reduced the GFAP expression 8%, there was no significant changes in NF-200 after treatment and no evident morphological changes with H and E staining. Conclusions With the above data we conclude that NaHS at 50 μM dose at 1 h after injury reduces the NO, LPO, GFAP and MPO level at injury site by increasing the expression of Nrf-2. We expect that a decrease in these parameters during acute phase of spinal cord injury would be helpful in neuroprotection and regeneration.

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