Effect of snuff and herpes simplex virus‐1 on rat oral mucosa: possible associations with the development of squamous cell carcinoma

J. ‐M Hirsch, S. L. Johansson, A. Vahlne

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The purpose of this investigation was to study the effect of snuff and experimentally induced herpes simplex virus type 1 (HSV‐1) infection in Sprague‐Dawley rats. It was demonstrated that it was possible to obtain 100% development of acute HSV‐1 infection in the rat oral mucosa, but only 10% of latent reactive infection of the trigeminal ganglia. The rats were, therefore, acutely infected monthly with virus to simulate recurrence of latent infection. Virus was applied topically to the mucous membrane twice with an interval of one month. Snuff was administered between the virus applications and afterwards to half the virus‐exposed animals. Sham‐infected rats were given snuff during the same period (18 months). A fourth group of rats were left untreated. A complete post‐mortem examination was performed. Two rats exposed to snuff and HSV‐1 in combination developed squamous cell carcinoma of the oral cavity. It was also found that rats exposed to snuff alone or in combination with HSV‐1 had a higher incidence of tumours or tumour‐like conditions than control rats exposed to HSV‐1 only. The incidence of malignant tumours was significantly higher in rats exposed to snuff or HSV‐1 and snuff in combination than in control animals (p < 0.05). The results of the study indicate that HSV‐1 in combination with snuff exposure may be associated with the development of squamous cell carcinomas of the oral cavity.

Original languageEnglish (US)
Pages (from-to)52-62
Number of pages11
JournalJournal of Oral Pathology & Medicine
Volume13
Issue number1
DOIs
StatePublished - Feb 1984

Fingerprint

Smokeless Tobacco
Herpes Simplex
Mouth Mucosa
Squamous Cell Carcinoma
Human Herpesvirus 1
Virus Diseases
Viruses
Mouth
Trigeminal Ganglion
Incidence
Infection
Autopsy
Neoplasms
Mucous Membrane
Recurrence

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Effect of snuff and herpes simplex virus‐1 on rat oral mucosa : possible associations with the development of squamous cell carcinoma. / Hirsch, J. ‐M; Johansson, S. L.; Vahlne, A.

In: Journal of Oral Pathology & Medicine, Vol. 13, No. 1, 02.1984, p. 52-62.

Research output: Contribution to journalArticle

@article{f352e578c9e7492dab3e5b0ca38d561f,
title = "Effect of snuff and herpes simplex virus‐1 on rat oral mucosa: possible associations with the development of squamous cell carcinoma",
abstract = "The purpose of this investigation was to study the effect of snuff and experimentally induced herpes simplex virus type 1 (HSV‐1) infection in Sprague‐Dawley rats. It was demonstrated that it was possible to obtain 100{\%} development of acute HSV‐1 infection in the rat oral mucosa, but only 10{\%} of latent reactive infection of the trigeminal ganglia. The rats were, therefore, acutely infected monthly with virus to simulate recurrence of latent infection. Virus was applied topically to the mucous membrane twice with an interval of one month. Snuff was administered between the virus applications and afterwards to half the virus‐exposed animals. Sham‐infected rats were given snuff during the same period (18 months). A fourth group of rats were left untreated. A complete post‐mortem examination was performed. Two rats exposed to snuff and HSV‐1 in combination developed squamous cell carcinoma of the oral cavity. It was also found that rats exposed to snuff alone or in combination with HSV‐1 had a higher incidence of tumours or tumour‐like conditions than control rats exposed to HSV‐1 only. The incidence of malignant tumours was significantly higher in rats exposed to snuff or HSV‐1 and snuff in combination than in control animals (p < 0.05). The results of the study indicate that HSV‐1 in combination with snuff exposure may be associated with the development of squamous cell carcinomas of the oral cavity.",
author = "Hirsch, {J. ‐M} and Johansson, {S. L.} and A. Vahlne",
year = "1984",
month = "2",
doi = "10.1111/j.1600-0714.1984.tb01400.x",
language = "English (US)",
volume = "13",
pages = "52--62",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Effect of snuff and herpes simplex virus‐1 on rat oral mucosa

T2 - possible associations with the development of squamous cell carcinoma

AU - Hirsch, J. ‐M

AU - Johansson, S. L.

AU - Vahlne, A.

PY - 1984/2

Y1 - 1984/2

N2 - The purpose of this investigation was to study the effect of snuff and experimentally induced herpes simplex virus type 1 (HSV‐1) infection in Sprague‐Dawley rats. It was demonstrated that it was possible to obtain 100% development of acute HSV‐1 infection in the rat oral mucosa, but only 10% of latent reactive infection of the trigeminal ganglia. The rats were, therefore, acutely infected monthly with virus to simulate recurrence of latent infection. Virus was applied topically to the mucous membrane twice with an interval of one month. Snuff was administered between the virus applications and afterwards to half the virus‐exposed animals. Sham‐infected rats were given snuff during the same period (18 months). A fourth group of rats were left untreated. A complete post‐mortem examination was performed. Two rats exposed to snuff and HSV‐1 in combination developed squamous cell carcinoma of the oral cavity. It was also found that rats exposed to snuff alone or in combination with HSV‐1 had a higher incidence of tumours or tumour‐like conditions than control rats exposed to HSV‐1 only. The incidence of malignant tumours was significantly higher in rats exposed to snuff or HSV‐1 and snuff in combination than in control animals (p < 0.05). The results of the study indicate that HSV‐1 in combination with snuff exposure may be associated with the development of squamous cell carcinomas of the oral cavity.

AB - The purpose of this investigation was to study the effect of snuff and experimentally induced herpes simplex virus type 1 (HSV‐1) infection in Sprague‐Dawley rats. It was demonstrated that it was possible to obtain 100% development of acute HSV‐1 infection in the rat oral mucosa, but only 10% of latent reactive infection of the trigeminal ganglia. The rats were, therefore, acutely infected monthly with virus to simulate recurrence of latent infection. Virus was applied topically to the mucous membrane twice with an interval of one month. Snuff was administered between the virus applications and afterwards to half the virus‐exposed animals. Sham‐infected rats were given snuff during the same period (18 months). A fourth group of rats were left untreated. A complete post‐mortem examination was performed. Two rats exposed to snuff and HSV‐1 in combination developed squamous cell carcinoma of the oral cavity. It was also found that rats exposed to snuff alone or in combination with HSV‐1 had a higher incidence of tumours or tumour‐like conditions than control rats exposed to HSV‐1 only. The incidence of malignant tumours was significantly higher in rats exposed to snuff or HSV‐1 and snuff in combination than in control animals (p < 0.05). The results of the study indicate that HSV‐1 in combination with snuff exposure may be associated with the development of squamous cell carcinomas of the oral cavity.

UR - http://www.scopus.com/inward/record.url?scp=0021382878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021382878&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0714.1984.tb01400.x

DO - 10.1111/j.1600-0714.1984.tb01400.x

M3 - Article

C2 - 6321709

AN - SCOPUS:0021382878

VL - 13

SP - 52

EP - 62

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 1

ER -