Effect of resveratrol on the expression of autocrine growth modulators in human breast cancer cells

G. Serrero, R. Lu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The effect of resveratrol on the growth of human breast cancer cells was examined. Resveratrol inhibited the growth of estrogen receptor-positive MCF-7 cells cultivated in the presence of estradiol in a dose-dependent fashion. At 10-5 M, resveratrol maximally inhibited the growth stimulatory effect mediated by 10-9 M estradiol without affecting cell viability. At the molecular level, resveratrol in a dose-dependent fashion antagonized the stimulation by estradiol of an estrogen response element reporter gene construct and of progesterone receptor gene expression in MCF-7 cells. Resveratrol also inhibited the proliferation of the estrogen-receptor negative human breast carcinoma cell line MDA-MB-468. These later data suggest that resveratrol can also inhibit breast cancer cell proliferation by another mechanism besides estrogen receptor antagonism. We show here that resveratrol altered the expression of several autocrine growth modulators and their receptors in MCF-7 cells. Resveratrol at 10-5 M inhibited the expression of the autocrine growth stimulators transforming growth factor-α (TGF-α), PC cell-derived growth factor, and insulin-like growth factor I receptor mRNA. In addition, resveratrol significantly elevated the expression of the growth inhibitor TGF-β2 mRNA without changes in TGF-β1 and TGF-β3 expression. These data suggest that resveratrol inhibits proliferation by altering autocrine growth modulator pathways in breast cancer cells.

Original languageEnglish (US)
Pages (from-to)969-979
Number of pages11
JournalAntioxidants and Redox Signaling
Volume3
Issue number6
DOIs
StatePublished - Jan 1 2001

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Modulators
Cells
Breast Neoplasms
Growth
Transforming Growth Factors
MCF-7 Cells
Estrogen Receptors
Estradiol
resveratrol
Growth Inhibitors
IGF Type 1 Receptor
Messenger RNA
Cell proliferation
Response Elements
Progesterone Receptors
Reporter Genes
Gene expression
Cell Survival
Estrogens
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Effect of resveratrol on the expression of autocrine growth modulators in human breast cancer cells. / Serrero, G.; Lu, R.

In: Antioxidants and Redox Signaling, Vol. 3, No. 6, 01.01.2001, p. 969-979.

Research output: Contribution to journalArticle

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