Effect of partially replacing a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows

E. Castillo-Lopez, J. Moats, N. D. Aluthge, H. A. Ramirez Ramirez, D. A. Christensen, T. Mutsvangwa, G. B. Penner, Samodha C Fernando

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: The effects of partial replacement of a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows were evaluated. Methods and Results: Treatments fed were CONT, a normal diet that included barley silage, alfalfa hay and a barley-based concentrate that contained no flaxseed or faba beans; FLAX, inclusion of a nonextruded flaxseed-based product containing 55·0% flaxseed, 37·8% field peas and 6·9% alfalfa; EXT, similar to FLAX, but the product was extruded and EXTT, similar to FLAX, but product was extruded and field peas were replaced by high-tannin faba beans. The rumen bacterial population was evaluated by utilizing 16S rRNA gene sequencing. Most abundant phyla, families and genera were unaffected. However, some taxa were affected; for example, unsaturated fatty acid content was negatively correlated with Clostridiaceae, and tannin content was negatively correlated with BS11 and Paraprevotellaceae. Conclusions: Predominant rumen bacterial taxa were not affected, but the abundance of some taxa found in lower proportions shifted, possibly due to sensitivity to unsaturated fatty acids or tannins. Significance and Impact of the Study: Flaxseed-based products were effective for partially replacing barley-based concentrate in rations of lactating dairy cows. No negative effects of these products were observed on the abundance of predominant rumen bacterial taxa, with only minor shifts in less abundant bacteria.

Original languageEnglish (US)
Pages (from-to)42-57
Number of pages16
JournalJournal of Applied Microbiology
Volume124
Issue number1
DOIs
StatePublished - Jan 2018

Fingerprint

Flax
Rumen
Hordeum
Tannins
Vicia faba
Medicago sativa
Population
Peas
Unsaturated Fatty Acids
Silage
rRNA Genes
Diet
Bacteria

Keywords

  • DNA sequencing
  • Holstein dairy cows
  • bacterial population
  • flaxseed
  • rumen

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Effect of partially replacing a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows. / Castillo-Lopez, E.; Moats, J.; Aluthge, N. D.; Ramirez Ramirez, H. A.; Christensen, D. A.; Mutsvangwa, T.; Penner, G. B.; Fernando, Samodha C.

In: Journal of Applied Microbiology, Vol. 124, No. 1, 01.2018, p. 42-57.

Research output: Contribution to journalArticle

Castillo-Lopez, E. ; Moats, J. ; Aluthge, N. D. ; Ramirez Ramirez, H. A. ; Christensen, D. A. ; Mutsvangwa, T. ; Penner, G. B. ; Fernando, Samodha C. / Effect of partially replacing a barley-based concentrate with flaxseed-based products on the rumen bacterial population of lactating Holstein dairy cows. In: Journal of Applied Microbiology. 2018 ; Vol. 124, No. 1. pp. 42-57.
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