Effect of papain digestion on the specificity of fluorescein-labeled immunoglobulins.

W. L. Mengeling, Prem S Paul

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During initial studies, we found that many fluorescein isothiocyanate-labeled anti-immunoglobulin conjugates were unstable and tended to aggregate and precipitate when used for indirect immunofluorescence microscopy. In some instances, the precipitate was extensive enough to interfere with interpretation of the test results. Attempts to resolve this problem resulted in a procedure by which such conjugates were digested with papain to Fab and Fc fragments before use. Aggregation and precipitation were prevented, while desired antibody activity was retained. Digestion with papain also reduced the diffuse background fluorescence (commonly referred to as nonspecific fluorescence or staining) that is often associated with conjugates before they are sorbed with tissue powders or chromatographed to remove highly labeled immunoglobulin molecules.

Original languageEnglish (US)
Pages (from-to)1235-1239
Number of pages5
JournalAmerican journal of veterinary research
Volume49
Issue number8
StatePublished - Jan 1 1988

Fingerprint

Papain
papain
fluorescein
Fluorescein
immunoglobulins
Immunoglobulins
Digestion
Fluorescence
digestion
fluorescence
Immunoglobulin Fc Fragments
Immunoglobulin Fab Fragments
isothiocyanates
protein aggregates
Indirect Fluorescent Antibody Technique
fluorescence microscopy
Fluorescence Microscopy
Powders
powders
Staining and Labeling

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Effect of papain digestion on the specificity of fluorescein-labeled immunoglobulins. / Mengeling, W. L.; Paul, Prem S.

In: American journal of veterinary research, Vol. 49, No. 8, 01.01.1988, p. 1235-1239.

Research output: Contribution to journalArticle

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