Effect of molecular crowding on the response of an electrochemical DNA sensor

Francesco Ricci, Rebecca Y. Lai, Alan J. Heeger, Kevin W. Plaxco, James J. Sumner

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

E-DNA sensors, the electrochemical equivalent of molecular beacons, appear to be a promising means of detecting oligonucleotides. E-DNA sensors are comprised of a redox-modified (here, methylene blue or ferrocene) DNA stemloop covalently attached to an interrogating electrode. Because E-DNA signaling arises due to binding-induced changes in the conformation of the stem-loop probe, it is likely sensitive to the nature of the molecular packing on the electrode surface. Here we detail the effects of probe density, target length, and other aspects of molecular crowding on the signaling properties, specificity, and response time of a model E-DNA sensor. We find that the highest signal suppression is obtained at the highest probe densities investigated, and that greater suppression is observed with longer and bulkier targets. In contrast, sensor equilibration time slows monotonically with increasing probe density, and the specificity of hybridization is not significantly affected. In addition to providing insight into the optimization of electrochemical DNA sensors, these results suggest that E-DNA signaling arises due to hybridization-linked changes in the rate, and thus efficiency, with which the redox moiety collides with the electrode and transfers electrons.

Original languageEnglish (US)
Pages (from-to)6827-6834
Number of pages8
JournalLangmuir
Volume23
Issue number12
DOIs
StatePublished - Jun 5 2007

Fingerprint

crowding
DNA
deoxyribonucleic acid
sensors
Sensors
probes
Electrodes
electrodes
retarding
beacons
oligonucleotides
Oligonucleotides
Methylene Blue
methylene blue
stems
Conformations
electron transfer
optimization
Electrons

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Ricci, F., Lai, R. Y., Heeger, A. J., Plaxco, K. W., & Sumner, J. J. (2007). Effect of molecular crowding on the response of an electrochemical DNA sensor. Langmuir, 23(12), 6827-6834. https://doi.org/10.1021/la700328r

Effect of molecular crowding on the response of an electrochemical DNA sensor. / Ricci, Francesco; Lai, Rebecca Y.; Heeger, Alan J.; Plaxco, Kevin W.; Sumner, James J.

In: Langmuir, Vol. 23, No. 12, 05.06.2007, p. 6827-6834.

Research output: Contribution to journalArticle

Ricci, F, Lai, RY, Heeger, AJ, Plaxco, KW & Sumner, JJ 2007, 'Effect of molecular crowding on the response of an electrochemical DNA sensor', Langmuir, vol. 23, no. 12, pp. 6827-6834. https://doi.org/10.1021/la700328r
Ricci, Francesco ; Lai, Rebecca Y. ; Heeger, Alan J. ; Plaxco, Kevin W. ; Sumner, James J. / Effect of molecular crowding on the response of an electrochemical DNA sensor. In: Langmuir. 2007 ; Vol. 23, No. 12. pp. 6827-6834.
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