Effect of milk on fibronectin and collagen type I binding to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis

J. Miedzobrodzki, A. S. Naidu, J. L. Watts, P. Ciborowski, K. Palm, T. Wadstrom

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Abstract

Tryptic soy broth (TSB)-grown cells of Staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly 125I-fibronectin (125I-Fn), whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with 125I-collagen (125I-Cn) type I. A particle agglutination assay (PAA) was used to examine the interaction-negative staphylococci with 125I-Fn and 125I-Cn immobilized on latex. All 368 coagulase-negative staphylococci demonstrated high 125I-Cn and moderate to low 125I-Fn interactions in the PAA. Cn-PAA reactivity was high among strains of Staphylococcus xylosus (84.2%), Staphylococcus simulans (77.8%), Staphylococcus epidermidis (76.7%), and Staphylococcus hyicus (74.3%), whereas all six Staphylococcus capitis strains clumped Cn-PAA reagent. Incubating TSB-grown cells in 10% skim milk for 1 h decreased the 125I-Fn- and 125I-Cn-binding affinity in most of the S. aureus and coagulase-negative staphylococci, while growth in 10% skim milk for 18 h resulted in more than 90% decrease or complete loss of interaction with these proteins. Decreased 125I-Fn binding in the presence of milk was correlated with protease production but not with 125I-Cn binding.

Original languageEnglish (US)
Pages (from-to)540-544
Number of pages5
JournalJournal of clinical microbiology
Volume27
Issue number3
StatePublished - Jan 1 1989

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Bovine Mastitis
Coagulase
Collagen Type I
Staphylococcus
Fibronectins
Staphylococcus aureus
Milk
Agglutination
Collagen
Staphylococcus hyicus
Staphylococcus epidermidis
Latex
Peptide Hydrolases
Growth

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Effect of milk on fibronectin and collagen type I binding to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis. / Miedzobrodzki, J.; Naidu, A. S.; Watts, J. L.; Ciborowski, P.; Palm, K.; Wadstrom, T.

In: Journal of clinical microbiology, Vol. 27, No. 3, 01.01.1989, p. 540-544.

Research output: Contribution to journalArticle

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abstract = "Tryptic soy broth (TSB)-grown cells of Staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly 125I-fibronectin (125I-Fn), whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with 125I-collagen (125I-Cn) type I. A particle agglutination assay (PAA) was used to examine the interaction-negative staphylococci with 125I-Fn and 125I-Cn immobilized on latex. All 368 coagulase-negative staphylococci demonstrated high 125I-Cn and moderate to low 125I-Fn interactions in the PAA. Cn-PAA reactivity was high among strains of Staphylococcus xylosus (84.2{\%}), Staphylococcus simulans (77.8{\%}), Staphylococcus epidermidis (76.7{\%}), and Staphylococcus hyicus (74.3{\%}), whereas all six Staphylococcus capitis strains clumped Cn-PAA reagent. Incubating TSB-grown cells in 10{\%} skim milk for 1 h decreased the 125I-Fn- and 125I-Cn-binding affinity in most of the S. aureus and coagulase-negative staphylococci, while growth in 10{\%} skim milk for 18 h resulted in more than 90{\%} decrease or complete loss of interaction with these proteins. Decreased 125I-Fn binding in the presence of milk was correlated with protease production but not with 125I-Cn binding.",
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