Effect of Loop Length and Sequence on the Stability of DNA Pyrimidine Triplexes with TAT Base Triplets

Hui Ting Lee, Carolyn E. Carr, Irine Khutsishvili, Luis A Marky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report the thermodynamic contributions of loop length and loop sequence to the overall stability of DNA intramolecular pyrimidine triplexes. Two sets of triplexes were designed: in the first set, the C5 loop closing the triplex stem was replaced with 5′-CTnC loops (n = 1-5), whereas in the second set, both the duplex and triplex loops were replaced with a 5′-GCAA or 5′-AACG tetraloop. For the triplexes with a 5′-CTnC loop, the triplex with five bases in the loop has the highest stability relative to the control. A loop length lower than five compromises the strength of the base-pair stacks without decreasing the thermal stability, leading to a decreased enthalpy, whereas an increase in the loop length leads to a decreased enthalpy and a higher entropic penalty. The incorporation of the GCAA loop yielded more stable triplexes, whereas the incorporation of AACG in the triplex loop yielded a less stable triplex due to an unfavorable enthalpy term. Thus, addition of the GCAA tetraloop can cause an increase in the thermodynamics of the triplex without affecting the sequence or melting behavior and may result in an additional layer of genetic regulation.

Original languageEnglish (US)
Pages (from-to)9175-9184
Number of pages10
JournalJournal of Physical Chemistry B
Volume121
Issue number39
DOIs
StatePublished - Oct 5 2017

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pyrimidines
Enthalpy
DNA
deoxyribonucleic acid
Thermodynamics
Melting
Thermodynamic stability
enthalpy
pyrimidine
thermodynamics
closing
penalties
stems
thermal stability
melting

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Effect of Loop Length and Sequence on the Stability of DNA Pyrimidine Triplexes with TAT Base Triplets. / Lee, Hui Ting; Carr, Carolyn E.; Khutsishvili, Irine; Marky, Luis A.

In: Journal of Physical Chemistry B, Vol. 121, No. 39, 05.10.2017, p. 9175-9184.

Research output: Contribution to journalArticle

Lee, Hui Ting ; Carr, Carolyn E. ; Khutsishvili, Irine ; Marky, Luis A. / Effect of Loop Length and Sequence on the Stability of DNA Pyrimidine Triplexes with TAT Base Triplets. In: Journal of Physical Chemistry B. 2017 ; Vol. 121, No. 39. pp. 9175-9184.
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