Effect of long‐term application of snuff on the oral mucosa

an experimental study in the rat

J. ‐M Hirsch, Sonny Lennart Johansson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The long‐term effect of snuff exposure was studied in Sprague‐Dawley rats using a surgically‐created test canal in the lower lip to retain snuff. The rats received standard snuff (n = 42) and highly alkaline snuff (n = 10) for 9–22 months, whereupon they were killed. Untreated rats with identical test canals (n = 15) served as controls. A complete post‐mortem examination was performed. One rat exposed to standard snuff for 9 months developed a squamous cell carcinoma of the oral cavity. However, exposure to standard snuff usually resulted in a mild to moderate hyperplasia of the epithelium, hyperorthokeratosis and acanthosis. Rats exposed to snuff for 18–22 months showed vacuolated cells penetrating deeper into the epithelium with hyperplastic and atrophic lesions. In a few rats, severe dysplastic changes developed in the crevicular epithelium. Rats exposed to alkaline snuff differed little from the first group except that there was focally atrophic and ulcerated epithelium and less fibrosis. Pathological findings outside the oral cavity were rare. Squamous cell hyperplasia of the forestomach was found in rats exposed to snuff for 18–22 months, possibly caused by ingested snuff. In conclusion, this study has shown that exposure of rats to snuff for 10–16 hours per day 5 days a week for most of their life‐span resulted in lesions mainly restricted to the epithelium and the underlying connective tissue of the surgically created test canal.

Original languageEnglish (US)
Pages (from-to)187-198
Number of pages12
JournalJournal of Oral Pathology & Medicine
Volume12
Issue number3
DOIs
StatePublished - Jan 1 1983

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Smokeless Tobacco
Mouth Mucosa
Epithelium
Hyperplasia
Mouth
Lip
Connective Tissue
Squamous Cell Carcinoma
Autopsy
Fibrosis
Epithelial Cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Effect of long‐term application of snuff on the oral mucosa : an experimental study in the rat. / Hirsch, J. ‐M; Johansson, Sonny Lennart.

In: Journal of Oral Pathology & Medicine, Vol. 12, No. 3, 01.01.1983, p. 187-198.

Research output: Contribution to journalArticle

Hirsch, J. ‐M ; Johansson, Sonny Lennart. / Effect of long‐term application of snuff on the oral mucosa : an experimental study in the rat. In: Journal of Oral Pathology & Medicine. 1983 ; Vol. 12, No. 3. pp. 187-198.
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