Effect of Lactobacillus acidophilus strain NP51 on Escherichia coli O157:H7 fecal shedding and finishing performance in beef feedlot cattle

R. E. Peterson, T. J. Klopfenstein, G. E. Erickson, J. Folmer, S. Hinkley, Rodney A Moxley, D. R. Smith

Research output: Contribution to journalArticle

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Abstract

A 2-year study was conducted during the summer months (May to September) to test the effectiveness of feeding Lactobacillus acidophilus strain NP51 on the proportion of cattle shedding Escherichia coli O157:H7 in the feces and evaluate the effect of the treatment on finishing performance. Steers (n = 448) were assigned randomly to pens, and pens of cattle were assigned randomly to NP51 supplementation or no supplementation (control). NP51 products were mixed with water and applied as the feed was mixed daily in treatment-designated trucks at the rate of 109 CFU per steer. Fecal samples were collected (n = 3,360) from the rectum from each animal every 3 weeks, and E. coli O157:H7 was isolated by standard procedures, using selective enrichment, immunomagnetic separation, and PCR confirmation. The outcome variable was the recovery of E. coli O157:H7 from feces, and was modeled using logistic regression accounting for year, repeated measures of pens of cattle, and block. No significant differences were detected for gain, intakes, or feed efficiency of control or NP51-fed steers. The probability for cattle to shed E. coli O157:H7 varied significantly between 2002 and 2003 (P = 0.004). In 2002 and 2003, the probability for NP51-treated steers to shed E. coli O157:H7 over the test periods was 13 and 21%, respectively, compared with 21 and 28% among controls. Over the 2 years, NP51-treated steers were 35% less likely to shed E. coli O157: H7 than were steers in untreated pens (odds ratio = 0.58, P = 0.008). This study is consistent with previous reports that feeding NP51 is effective in reducing E. coli O157:H7 fecal shedding in feedlot cattle.

Original languageEnglish (US)
Pages (from-to)287-291
Number of pages5
JournalJournal of food protection
Volume70
Issue number2
DOIs
StatePublished - Jan 1 2007

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Lactobacillus acidophilus
Escherichia coli O157
feedlots
finishing
beef
cattle
Feces
feces
Immunomagnetic Separation
immunomagnetic separation
trucks
Motor Vehicles
rectum
Red Meat
Rectum
odds ratio
feed conversion
Logistic Models
Odds Ratio
testing

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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Effect of Lactobacillus acidophilus strain NP51 on Escherichia coli O157:H7 fecal shedding and finishing performance in beef feedlot cattle. / Peterson, R. E.; Klopfenstein, T. J.; Erickson, G. E.; Folmer, J.; Hinkley, S.; Moxley, Rodney A; Smith, D. R.

In: Journal of food protection, Vol. 70, No. 2, 01.01.2007, p. 287-291.

Research output: Contribution to journalArticle

Peterson, R. E. ; Klopfenstein, T. J. ; Erickson, G. E. ; Folmer, J. ; Hinkley, S. ; Moxley, Rodney A ; Smith, D. R. / Effect of Lactobacillus acidophilus strain NP51 on Escherichia coli O157:H7 fecal shedding and finishing performance in beef feedlot cattle. In: Journal of food protection. 2007 ; Vol. 70, No. 2. pp. 287-291.
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