Effect of Insulin on Glucose Uptake in Near-Term Fetal Lambs

William W. Hay, Huei Kang Meznarich, John W. Sparks, Frederick C. Battaglia, Giacomo Meschia

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Glucose clamp experiments were performed in 27 chronically catheterized, late-gestation fetal lambs in order to measure the effect of fetal insulin concentration on fetal glucose uptake at a constant glucose concentration. Fetal arterial blood glucose concentration was measured over a 30-min control period and then maintained at the control value by a variable glucose infusion into the fetus while insulin was infused at a constant rate into the fetus. Plasma insulin concentration increased from 21 ± 10 (SD) to 294 ± 179 (SD) μU·ml−1. The exogenous glucose infusion rate necessary to maintain constant glycemia during the plateau hyperinsulinemia averaged 4.3 ± 1.6 (SD) mg·min−1 ·kg−1. In a subset of 13 animals, total fetal exogenous glucose uptake (FGU; sum of glucose uptake from the placenta via the umbilical circulation plus the steady-state exogenous glucose infusion rate) was measured during the control and hyperinsulinemia period. FGU was directly related to insulin concentration (y = 4.24 + 0.07x) at insulin levels < 100 μU/ml and increased 132% above control at insulin levels above 100 μU/ml. Hyperinsulinemia did not affect fetal glucose uptake from the placenta via the umbilical circulation. These studies demonstrate that insulin concentration is a major factor controlling glucose uptake in the near-term fetal lamb, and that an increase of fetal insulin does not affect the transport of glucose to the fetus from the placenta.

Original languageEnglish (US)
Pages (from-to)557-564
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Volume178
Issue number4
DOIs
StatePublished - Apr 1985

Fingerprint

Insulin
Glucose
Hyperinsulinism
Placenta
Umbilicus
Fetus
Glucose Clamp Technique
Clamping devices
Fetal Blood
Blood Glucose
Animals
Pregnancy
Plasmas

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effect of Insulin on Glucose Uptake in Near-Term Fetal Lambs. / Hay, William W.; Meznarich, Huei Kang; Sparks, John W.; Battaglia, Frederick C.; Meschia, Giacomo.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 178, No. 4, 04.1985, p. 557-564.

Research output: Contribution to journalArticle

Hay, William W. ; Meznarich, Huei Kang ; Sparks, John W. ; Battaglia, Frederick C. ; Meschia, Giacomo. / Effect of Insulin on Glucose Uptake in Near-Term Fetal Lambs. In: Proceedings of the Society for Experimental Biology and Medicine. 1985 ; Vol. 178, No. 4. pp. 557-564.
@article{649b829de3f24ec9951ec78d17313862,
title = "Effect of Insulin on Glucose Uptake in Near-Term Fetal Lambs",
abstract = "Glucose clamp experiments were performed in 27 chronically catheterized, late-gestation fetal lambs in order to measure the effect of fetal insulin concentration on fetal glucose uptake at a constant glucose concentration. Fetal arterial blood glucose concentration was measured over a 30-min control period and then maintained at the control value by a variable glucose infusion into the fetus while insulin was infused at a constant rate into the fetus. Plasma insulin concentration increased from 21 ± 10 (SD) to 294 ± 179 (SD) μU·ml−1. The exogenous glucose infusion rate necessary to maintain constant glycemia during the plateau hyperinsulinemia averaged 4.3 ± 1.6 (SD) mg·min−1 ·kg−1. In a subset of 13 animals, total fetal exogenous glucose uptake (FGU; sum of glucose uptake from the placenta via the umbilical circulation plus the steady-state exogenous glucose infusion rate) was measured during the control and hyperinsulinemia period. FGU was directly related to insulin concentration (y = 4.24 + 0.07x) at insulin levels < 100 μU/ml and increased 132{\%} above control at insulin levels above 100 μU/ml. Hyperinsulinemia did not affect fetal glucose uptake from the placenta via the umbilical circulation. These studies demonstrate that insulin concentration is a major factor controlling glucose uptake in the near-term fetal lamb, and that an increase of fetal insulin does not affect the transport of glucose to the fetus from the placenta.",
author = "Hay, {William W.} and Meznarich, {Huei Kang} and Sparks, {John W.} and Battaglia, {Frederick C.} and Giacomo Meschia",
year = "1985",
month = "4",
doi = "10.3181/00379727-178-42042",
language = "English (US)",
volume = "178",
pages = "557--564",
journal = "Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)",
issn = "1535-3702",
publisher = "Society for Experimental Biology and Medicine",
number = "4",

}

TY - JOUR

T1 - Effect of Insulin on Glucose Uptake in Near-Term Fetal Lambs

AU - Hay, William W.

AU - Meznarich, Huei Kang

AU - Sparks, John W.

AU - Battaglia, Frederick C.

AU - Meschia, Giacomo

PY - 1985/4

Y1 - 1985/4

N2 - Glucose clamp experiments were performed in 27 chronically catheterized, late-gestation fetal lambs in order to measure the effect of fetal insulin concentration on fetal glucose uptake at a constant glucose concentration. Fetal arterial blood glucose concentration was measured over a 30-min control period and then maintained at the control value by a variable glucose infusion into the fetus while insulin was infused at a constant rate into the fetus. Plasma insulin concentration increased from 21 ± 10 (SD) to 294 ± 179 (SD) μU·ml−1. The exogenous glucose infusion rate necessary to maintain constant glycemia during the plateau hyperinsulinemia averaged 4.3 ± 1.6 (SD) mg·min−1 ·kg−1. In a subset of 13 animals, total fetal exogenous glucose uptake (FGU; sum of glucose uptake from the placenta via the umbilical circulation plus the steady-state exogenous glucose infusion rate) was measured during the control and hyperinsulinemia period. FGU was directly related to insulin concentration (y = 4.24 + 0.07x) at insulin levels < 100 μU/ml and increased 132% above control at insulin levels above 100 μU/ml. Hyperinsulinemia did not affect fetal glucose uptake from the placenta via the umbilical circulation. These studies demonstrate that insulin concentration is a major factor controlling glucose uptake in the near-term fetal lamb, and that an increase of fetal insulin does not affect the transport of glucose to the fetus from the placenta.

AB - Glucose clamp experiments were performed in 27 chronically catheterized, late-gestation fetal lambs in order to measure the effect of fetal insulin concentration on fetal glucose uptake at a constant glucose concentration. Fetal arterial blood glucose concentration was measured over a 30-min control period and then maintained at the control value by a variable glucose infusion into the fetus while insulin was infused at a constant rate into the fetus. Plasma insulin concentration increased from 21 ± 10 (SD) to 294 ± 179 (SD) μU·ml−1. The exogenous glucose infusion rate necessary to maintain constant glycemia during the plateau hyperinsulinemia averaged 4.3 ± 1.6 (SD) mg·min−1 ·kg−1. In a subset of 13 animals, total fetal exogenous glucose uptake (FGU; sum of glucose uptake from the placenta via the umbilical circulation plus the steady-state exogenous glucose infusion rate) was measured during the control and hyperinsulinemia period. FGU was directly related to insulin concentration (y = 4.24 + 0.07x) at insulin levels < 100 μU/ml and increased 132% above control at insulin levels above 100 μU/ml. Hyperinsulinemia did not affect fetal glucose uptake from the placenta via the umbilical circulation. These studies demonstrate that insulin concentration is a major factor controlling glucose uptake in the near-term fetal lamb, and that an increase of fetal insulin does not affect the transport of glucose to the fetus from the placenta.

UR - http://www.scopus.com/inward/record.url?scp=0021839999&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021839999&partnerID=8YFLogxK

U2 - 10.3181/00379727-178-42042

DO - 10.3181/00379727-178-42042

M3 - Article

C2 - 3885234

AN - SCOPUS:0021839999

VL - 178

SP - 557

EP - 564

JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

SN - 1535-3702

IS - 4

ER -