Effect of elevated carbon dioxide on bronchial epithelial innate immune receptor response to organic dust from swine confinement barns

D. Schneberger, D. Cloonan, J. M. Devasure, Kristina L Bailey, Debra Romberger, Todd A Wyatt

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Abstract Hypercapnia is known to have immunoregulatory effects within the lung. Cell culture systems demonstrate this in both macrophages and alveolar cell lines, suggesting that the alveoli are affected by changes in CO2 levels. We hypothesized that hypercapnia would also modulate human bronchial epithelial cell immune responses. Innate immune responses to Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand) and a complex innate immune stimulus, an extract from the organic dust of swine confinement barns (barn dust extract or BDE), were tested in a human bronchial epithelial cell line, BEAS-2B. Both TLR ligands showed a decrease in IL-6 and IL-8 production, and an increase in MCP-1 in response to elevated CO2 indicating an enhancement in cytokine production to hypercapnia. This change was not reflected in expression levels of TLR receptor RNA which remained unchanged in response to elevated CO2. Interestingly, barn dust showed an increase in IL-6, IL-8 and MCP-1 response at 9% CO2, suggesting that elevated CO2 exerts different effects on different stimuli. Our results show that airway epithelial cell immune responses to barn dust respond differently to hypercapnic conditions than individual TLR ligands.

Original languageEnglish (US)
Article number3667
Pages (from-to)76-84
Number of pages9
JournalInternational Immunopharmacology
Volume27
Issue number1
DOIs
StatePublished - Apr 25 2015

Fingerprint

Dust
Innate Immunity
Carbon Dioxide
Hypercapnia
Swine
Ligands
Epithelial Cells
Interleukin-8
Interleukin-6
Alveolar Epithelial Cells
Cell Line
Antigen-Antibody Complex
Cell Culture Techniques
Macrophages
RNA
Cytokines
Lung

Keywords

  • Barn dust
  • Bronchial epithelium
  • Carbon dioxide
  • Hypercapnia
  • LPS
  • Lung
  • TLR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Effect of elevated carbon dioxide on bronchial epithelial innate immune receptor response to organic dust from swine confinement barns. / Schneberger, D.; Cloonan, D.; Devasure, J. M.; Bailey, Kristina L; Romberger, Debra; Wyatt, Todd A.

In: International Immunopharmacology, Vol. 27, No. 1, 3667, 25.04.2015, p. 76-84.

Research output: Contribution to journalArticle

@article{cbb8093b2a8c471bb9865d822b47ba6e,
title = "Effect of elevated carbon dioxide on bronchial epithelial innate immune receptor response to organic dust from swine confinement barns",
abstract = "Abstract Hypercapnia is known to have immunoregulatory effects within the lung. Cell culture systems demonstrate this in both macrophages and alveolar cell lines, suggesting that the alveoli are affected by changes in CO2 levels. We hypothesized that hypercapnia would also modulate human bronchial epithelial cell immune responses. Innate immune responses to Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand) and a complex innate immune stimulus, an extract from the organic dust of swine confinement barns (barn dust extract or BDE), were tested in a human bronchial epithelial cell line, BEAS-2B. Both TLR ligands showed a decrease in IL-6 and IL-8 production, and an increase in MCP-1 in response to elevated CO2 indicating an enhancement in cytokine production to hypercapnia. This change was not reflected in expression levels of TLR receptor RNA which remained unchanged in response to elevated CO2. Interestingly, barn dust showed an increase in IL-6, IL-8 and MCP-1 response at 9{\%} CO2, suggesting that elevated CO2 exerts different effects on different stimuli. Our results show that airway epithelial cell immune responses to barn dust respond differently to hypercapnic conditions than individual TLR ligands.",
keywords = "Barn dust, Bronchial epithelium, Carbon dioxide, Hypercapnia, LPS, Lung, TLR",
author = "D. Schneberger and D. Cloonan and Devasure, {J. M.} and Bailey, {Kristina L} and Debra Romberger and Wyatt, {Todd A}",
year = "2015",
month = "4",
day = "25",
doi = "10.1016/j.intimp.2015.04.031",
language = "English (US)",
volume = "27",
pages = "76--84",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Effect of elevated carbon dioxide on bronchial epithelial innate immune receptor response to organic dust from swine confinement barns

AU - Schneberger, D.

AU - Cloonan, D.

AU - Devasure, J. M.

AU - Bailey, Kristina L

AU - Romberger, Debra

AU - Wyatt, Todd A

PY - 2015/4/25

Y1 - 2015/4/25

N2 - Abstract Hypercapnia is known to have immunoregulatory effects within the lung. Cell culture systems demonstrate this in both macrophages and alveolar cell lines, suggesting that the alveoli are affected by changes in CO2 levels. We hypothesized that hypercapnia would also modulate human bronchial epithelial cell immune responses. Innate immune responses to Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand) and a complex innate immune stimulus, an extract from the organic dust of swine confinement barns (barn dust extract or BDE), were tested in a human bronchial epithelial cell line, BEAS-2B. Both TLR ligands showed a decrease in IL-6 and IL-8 production, and an increase in MCP-1 in response to elevated CO2 indicating an enhancement in cytokine production to hypercapnia. This change was not reflected in expression levels of TLR receptor RNA which remained unchanged in response to elevated CO2. Interestingly, barn dust showed an increase in IL-6, IL-8 and MCP-1 response at 9% CO2, suggesting that elevated CO2 exerts different effects on different stimuli. Our results show that airway epithelial cell immune responses to barn dust respond differently to hypercapnic conditions than individual TLR ligands.

AB - Abstract Hypercapnia is known to have immunoregulatory effects within the lung. Cell culture systems demonstrate this in both macrophages and alveolar cell lines, suggesting that the alveoli are affected by changes in CO2 levels. We hypothesized that hypercapnia would also modulate human bronchial epithelial cell immune responses. Innate immune responses to Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand) and a complex innate immune stimulus, an extract from the organic dust of swine confinement barns (barn dust extract or BDE), were tested in a human bronchial epithelial cell line, BEAS-2B. Both TLR ligands showed a decrease in IL-6 and IL-8 production, and an increase in MCP-1 in response to elevated CO2 indicating an enhancement in cytokine production to hypercapnia. This change was not reflected in expression levels of TLR receptor RNA which remained unchanged in response to elevated CO2. Interestingly, barn dust showed an increase in IL-6, IL-8 and MCP-1 response at 9% CO2, suggesting that elevated CO2 exerts different effects on different stimuli. Our results show that airway epithelial cell immune responses to barn dust respond differently to hypercapnic conditions than individual TLR ligands.

KW - Barn dust

KW - Bronchial epithelium

KW - Carbon dioxide

KW - Hypercapnia

KW - LPS

KW - Lung

KW - TLR

UR - http://www.scopus.com/inward/record.url?scp=84929149510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929149510&partnerID=8YFLogxK

U2 - 10.1016/j.intimp.2015.04.031

DO - 10.1016/j.intimp.2015.04.031

M3 - Article

VL - 27

SP - 76

EP - 84

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 1

M1 - 3667

ER -