Effect of dose on the induction of urothelial proliferation by N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide and its relationship to bladder carcinogenesis in the rat

R. Hasegawa, Samuel Monroe Cohen, M. St John, M. Cano, L. B. Ellwein

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect on urothelial proliferation of a urinary bladder carcinogen, N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide (FANFT), fed to male F344 rats at doses of 0.2, 0.1, 0.05, 0.01, 0.005 and 0.001% of diet for 4 or 10 weeks was evaluated by autoradiography, using (3H-methyl)thymidine, and by histopathology. At week 4, hyperplasia was induced in 10/11 and 6/11 rats given 0.2% and 0.1% FANFT, respectively. The dose-related increase of labeling index in the bladder epithelium was significant for the groups given 0.01% or higher doses of FANFT. At week 10, histopathologic lesions were observed in groups given 0.05% or higher doses of FANFT. This was accompanied by a significant increase in labeling index for these groups. The results are consistent with the long-term carcinogenicity studies conducted with the same dose levels of FANFT. The interrelationships between numbers of cells (hyperplasia), cell proliferation (labeling index) and cancer induction are discussed utilizing a computerized model of bladder carcinogenesis.

Original languageEnglish (US)
Pages (from-to)633-636
Number of pages4
JournalCarcinogenesis
Volume7
Issue number4
DOIs
StatePublished - Dec 1 1986

Fingerprint

FANFT
Carcinogenesis
Urinary Bladder
Hyperplasia
Inbred F344 Rats
Autoradiography
Carcinogens
Computer Simulation
Thymidine
Epithelium
Cell Count
Cell Proliferation
formamide
Diet

ASJC Scopus subject areas

  • Cancer Research

Cite this

Effect of dose on the induction of urothelial proliferation by N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide and its relationship to bladder carcinogenesis in the rat. / Hasegawa, R.; Cohen, Samuel Monroe; St John, M.; Cano, M.; Ellwein, L. B.

In: Carcinogenesis, Vol. 7, No. 4, 01.12.1986, p. 633-636.

Research output: Contribution to journalArticle

@article{c8384903675042189aa35e0b99e4ce50,
title = "Effect of dose on the induction of urothelial proliferation by N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide and its relationship to bladder carcinogenesis in the rat",
abstract = "The effect on urothelial proliferation of a urinary bladder carcinogen, N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide (FANFT), fed to male F344 rats at doses of 0.2, 0.1, 0.05, 0.01, 0.005 and 0.001{\%} of diet for 4 or 10 weeks was evaluated by autoradiography, using (3H-methyl)thymidine, and by histopathology. At week 4, hyperplasia was induced in 10/11 and 6/11 rats given 0.2{\%} and 0.1{\%} FANFT, respectively. The dose-related increase of labeling index in the bladder epithelium was significant for the groups given 0.01{\%} or higher doses of FANFT. At week 10, histopathologic lesions were observed in groups given 0.05{\%} or higher doses of FANFT. This was accompanied by a significant increase in labeling index for these groups. The results are consistent with the long-term carcinogenicity studies conducted with the same dose levels of FANFT. The interrelationships between numbers of cells (hyperplasia), cell proliferation (labeling index) and cancer induction are discussed utilizing a computerized model of bladder carcinogenesis.",
author = "R. Hasegawa and Cohen, {Samuel Monroe} and {St John}, M. and M. Cano and Ellwein, {L. B.}",
year = "1986",
month = "12",
day = "1",
doi = "10.1093/carcin/7.4.633",
language = "English (US)",
volume = "7",
pages = "633--636",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Effect of dose on the induction of urothelial proliferation by N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide and its relationship to bladder carcinogenesis in the rat

AU - Hasegawa, R.

AU - Cohen, Samuel Monroe

AU - St John, M.

AU - Cano, M.

AU - Ellwein, L. B.

PY - 1986/12/1

Y1 - 1986/12/1

N2 - The effect on urothelial proliferation of a urinary bladder carcinogen, N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide (FANFT), fed to male F344 rats at doses of 0.2, 0.1, 0.05, 0.01, 0.005 and 0.001% of diet for 4 or 10 weeks was evaluated by autoradiography, using (3H-methyl)thymidine, and by histopathology. At week 4, hyperplasia was induced in 10/11 and 6/11 rats given 0.2% and 0.1% FANFT, respectively. The dose-related increase of labeling index in the bladder epithelium was significant for the groups given 0.01% or higher doses of FANFT. At week 10, histopathologic lesions were observed in groups given 0.05% or higher doses of FANFT. This was accompanied by a significant increase in labeling index for these groups. The results are consistent with the long-term carcinogenicity studies conducted with the same dose levels of FANFT. The interrelationships between numbers of cells (hyperplasia), cell proliferation (labeling index) and cancer induction are discussed utilizing a computerized model of bladder carcinogenesis.

AB - The effect on urothelial proliferation of a urinary bladder carcinogen, N-(4-(5-nitro-2-furyl)-2-thiazolyl)formamide (FANFT), fed to male F344 rats at doses of 0.2, 0.1, 0.05, 0.01, 0.005 and 0.001% of diet for 4 or 10 weeks was evaluated by autoradiography, using (3H-methyl)thymidine, and by histopathology. At week 4, hyperplasia was induced in 10/11 and 6/11 rats given 0.2% and 0.1% FANFT, respectively. The dose-related increase of labeling index in the bladder epithelium was significant for the groups given 0.01% or higher doses of FANFT. At week 10, histopathologic lesions were observed in groups given 0.05% or higher doses of FANFT. This was accompanied by a significant increase in labeling index for these groups. The results are consistent with the long-term carcinogenicity studies conducted with the same dose levels of FANFT. The interrelationships between numbers of cells (hyperplasia), cell proliferation (labeling index) and cancer induction are discussed utilizing a computerized model of bladder carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0023034115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023034115&partnerID=8YFLogxK

U2 - 10.1093/carcin/7.4.633

DO - 10.1093/carcin/7.4.633

M3 - Article

VL - 7

SP - 633

EP - 636

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 4

ER -