Effect of Direct-Fed Microbial Dosage on the Fecal Concentrations of Enterohemorrhagic Escherichia coli in Feedlot Cattle

Brandon E. Luedtke, Joseph M. Bosilevac, Dayna M. Harhay, Terrance M. Arthur

Research output: Contribution to journalArticle

Abstract

Contamination of beef products by Shiga toxin-producing Escherichia coli is a concern for food safety with a particular subset, the enterohemorrhagic E. coli (EHEC), being the most relevant to human disease. To mitigate food safety risks, preharvest intervention strategies have been implemented with the aim to reduce EHEC in cattle. One class of interventions that has been widely used in feedlots is direct-fed microbials (DFMs), which can contain various dosing rates of probiotic bacteria. Here we compare the use of two different doses of a commercially available DFM on total EHEC load in a commercial feedlot setting. The DFMs used were the standard 109 Propionibacterium freudenreichii and 106 Lactobacillus acidophilus colony forming units (CFUs)/head/day dose of Bovamine® (Nutrition Physiology Company, Guymon, OK) and the higher dose, Bovamine Defend™ (Nutrition Physiology Company), which is dosed at 109 P. freudenreichii and 109 Lactobacillus acidophilus CFUs/head/day. To analyze the total EHEC fecal concentration, 2200 head of cattle were assigned a DFM feed regimen lasting approximately 5 months. At harvest, 480 head of cattle were sampled using rectoanal mucosal swabs. A quantitative polymerase chain reaction assay targeting ecf1 was used to enumerate the total EHEC fecal concentration for 240 head fed the low-dose DFM and 240 head fed the high-dose DFM. No significant difference (p > 0.05) in the fecal concentration of total EHEC was observed between the two doses. This suggests that using an increased dosage provides no additional reduction in the total EHEC fecal concentration of feedlot cattle compared to the standard dosage.

Original languageEnglish (US)
Pages (from-to)190-195
Number of pages6
JournalFoodborne pathogens and disease
Volume13
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

Enterohemorrhagic Escherichia coli
enterohemorrhagic Escherichia coli
feedlots
cattle
dosage
Nutritional Physiological Phenomena
Head
Lactobacillus acidophilus
nutrition physiology
Food Safety
Propionibacterium freudenreichii
Stem Cells
food safety
Shiga-Toxigenic Escherichia coli
Probiotics
Shiga toxin-producing Escherichia coli
human diseases
probiotics
Bacteria
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

Cite this

Effect of Direct-Fed Microbial Dosage on the Fecal Concentrations of Enterohemorrhagic Escherichia coli in Feedlot Cattle. / Luedtke, Brandon E.; Bosilevac, Joseph M.; Harhay, Dayna M.; Arthur, Terrance M.

In: Foodborne pathogens and disease, Vol. 13, No. 4, 01.04.2016, p. 190-195.

Research output: Contribution to journalArticle

@article{1dace8c103e3466b914d069b1423a1ca,
title = "Effect of Direct-Fed Microbial Dosage on the Fecal Concentrations of Enterohemorrhagic Escherichia coli in Feedlot Cattle",
abstract = "Contamination of beef products by Shiga toxin-producing Escherichia coli is a concern for food safety with a particular subset, the enterohemorrhagic E. coli (EHEC), being the most relevant to human disease. To mitigate food safety risks, preharvest intervention strategies have been implemented with the aim to reduce EHEC in cattle. One class of interventions that has been widely used in feedlots is direct-fed microbials (DFMs), which can contain various dosing rates of probiotic bacteria. Here we compare the use of two different doses of a commercially available DFM on total EHEC load in a commercial feedlot setting. The DFMs used were the standard 109 Propionibacterium freudenreichii and 106 Lactobacillus acidophilus colony forming units (CFUs)/head/day dose of Bovamine{\circledR} (Nutrition Physiology Company, Guymon, OK) and the higher dose, Bovamine Defend™ (Nutrition Physiology Company), which is dosed at 109 P. freudenreichii and 109 Lactobacillus acidophilus CFUs/head/day. To analyze the total EHEC fecal concentration, 2200 head of cattle were assigned a DFM feed regimen lasting approximately 5 months. At harvest, 480 head of cattle were sampled using rectoanal mucosal swabs. A quantitative polymerase chain reaction assay targeting ecf1 was used to enumerate the total EHEC fecal concentration for 240 head fed the low-dose DFM and 240 head fed the high-dose DFM. No significant difference (p > 0.05) in the fecal concentration of total EHEC was observed between the two doses. This suggests that using an increased dosage provides no additional reduction in the total EHEC fecal concentration of feedlot cattle compared to the standard dosage.",
author = "Luedtke, {Brandon E.} and Bosilevac, {Joseph M.} and Harhay, {Dayna M.} and Arthur, {Terrance M.}",
year = "2016",
month = "4",
day = "1",
doi = "10.1089/fpd.2015.2063",
language = "English (US)",
volume = "13",
pages = "190--195",
journal = "Foodborne Pathogens and Disease",
issn = "1535-3141",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Effect of Direct-Fed Microbial Dosage on the Fecal Concentrations of Enterohemorrhagic Escherichia coli in Feedlot Cattle

AU - Luedtke, Brandon E.

AU - Bosilevac, Joseph M.

AU - Harhay, Dayna M.

AU - Arthur, Terrance M.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Contamination of beef products by Shiga toxin-producing Escherichia coli is a concern for food safety with a particular subset, the enterohemorrhagic E. coli (EHEC), being the most relevant to human disease. To mitigate food safety risks, preharvest intervention strategies have been implemented with the aim to reduce EHEC in cattle. One class of interventions that has been widely used in feedlots is direct-fed microbials (DFMs), which can contain various dosing rates of probiotic bacteria. Here we compare the use of two different doses of a commercially available DFM on total EHEC load in a commercial feedlot setting. The DFMs used were the standard 109 Propionibacterium freudenreichii and 106 Lactobacillus acidophilus colony forming units (CFUs)/head/day dose of Bovamine® (Nutrition Physiology Company, Guymon, OK) and the higher dose, Bovamine Defend™ (Nutrition Physiology Company), which is dosed at 109 P. freudenreichii and 109 Lactobacillus acidophilus CFUs/head/day. To analyze the total EHEC fecal concentration, 2200 head of cattle were assigned a DFM feed regimen lasting approximately 5 months. At harvest, 480 head of cattle were sampled using rectoanal mucosal swabs. A quantitative polymerase chain reaction assay targeting ecf1 was used to enumerate the total EHEC fecal concentration for 240 head fed the low-dose DFM and 240 head fed the high-dose DFM. No significant difference (p > 0.05) in the fecal concentration of total EHEC was observed between the two doses. This suggests that using an increased dosage provides no additional reduction in the total EHEC fecal concentration of feedlot cattle compared to the standard dosage.

AB - Contamination of beef products by Shiga toxin-producing Escherichia coli is a concern for food safety with a particular subset, the enterohemorrhagic E. coli (EHEC), being the most relevant to human disease. To mitigate food safety risks, preharvest intervention strategies have been implemented with the aim to reduce EHEC in cattle. One class of interventions that has been widely used in feedlots is direct-fed microbials (DFMs), which can contain various dosing rates of probiotic bacteria. Here we compare the use of two different doses of a commercially available DFM on total EHEC load in a commercial feedlot setting. The DFMs used were the standard 109 Propionibacterium freudenreichii and 106 Lactobacillus acidophilus colony forming units (CFUs)/head/day dose of Bovamine® (Nutrition Physiology Company, Guymon, OK) and the higher dose, Bovamine Defend™ (Nutrition Physiology Company), which is dosed at 109 P. freudenreichii and 109 Lactobacillus acidophilus CFUs/head/day. To analyze the total EHEC fecal concentration, 2200 head of cattle were assigned a DFM feed regimen lasting approximately 5 months. At harvest, 480 head of cattle were sampled using rectoanal mucosal swabs. A quantitative polymerase chain reaction assay targeting ecf1 was used to enumerate the total EHEC fecal concentration for 240 head fed the low-dose DFM and 240 head fed the high-dose DFM. No significant difference (p > 0.05) in the fecal concentration of total EHEC was observed between the two doses. This suggests that using an increased dosage provides no additional reduction in the total EHEC fecal concentration of feedlot cattle compared to the standard dosage.

UR - http://www.scopus.com/inward/record.url?scp=84964573694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964573694&partnerID=8YFLogxK

U2 - 10.1089/fpd.2015.2063

DO - 10.1089/fpd.2015.2063

M3 - Article

C2 - 26974651

AN - SCOPUS:84964573694

VL - 13

SP - 190

EP - 195

JO - Foodborne Pathogens and Disease

JF - Foodborne Pathogens and Disease

SN - 1535-3141

IS - 4

ER -