Effect of culture conditions on Escherichia coli O157:H7-mediated attaching-effacing lesions in a bovine large intestinal mucosal explant model

Angela A. Baehler, Rodney A Moxley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effects of culture conditions on the extent of Escherichia coli O157:H7 attaching-effacing (A/E) adherence in an adult bovine large intestinal mucosal explant model were assessed by three different morphometric methods. Measurement of the percent of tissue sections with A/E adherence and the number of foci of A/E adherence mm-1 of surface epithelium was more sensitive than measurement of the percent of surface epithelium with A/E adherent bacteria for detection of treatment effects. Culture of bacterial inoculum in tryptic soy broth, incubation of explants in 5% CO2, and rocking of explants on a platform rocker at 18 cycles min-1 provided optimal conditions for A/E adherence. In future studies, the model may be used for preliminary testing of intervention strategies aimed at reduction of E. coli O157:H7 intestinal colonization of cattle.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalFEMS Microbiology Letters
Volume212
Issue number1
DOIs
StatePublished - Jun 18 2002

Fingerprint

Escherichia coli O157
Epithelium
Bacteria
trypticase-soy broth

Keywords

  • Attaching-effacing
  • Cattle
  • Escherichia coli O157:H7
  • Explant
  • Large intestine
  • Morphometry

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

@article{610cb33c43e441fc9a7a78650448293e,
title = "Effect of culture conditions on Escherichia coli O157:H7-mediated attaching-effacing lesions in a bovine large intestinal mucosal explant model",
abstract = "The effects of culture conditions on the extent of Escherichia coli O157:H7 attaching-effacing (A/E) adherence in an adult bovine large intestinal mucosal explant model were assessed by three different morphometric methods. Measurement of the percent of tissue sections with A/E adherence and the number of foci of A/E adherence mm-1 of surface epithelium was more sensitive than measurement of the percent of surface epithelium with A/E adherent bacteria for detection of treatment effects. Culture of bacterial inoculum in tryptic soy broth, incubation of explants in 5{\%} CO2, and rocking of explants on a platform rocker at 18 cycles min-1 provided optimal conditions for A/E adherence. In future studies, the model may be used for preliminary testing of intervention strategies aimed at reduction of E. coli O157:H7 intestinal colonization of cattle.",
keywords = "Attaching-effacing, Cattle, Escherichia coli O157:H7, Explant, Large intestine, Morphometry",
author = "Baehler, {Angela A.} and Moxley, {Rodney A}",
year = "2002",
month = "6",
day = "18",
doi = "10.1016/S0378-1097(02)00718-8",
language = "English (US)",
volume = "212",
pages = "107--110",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Effect of culture conditions on Escherichia coli O157:H7-mediated attaching-effacing lesions in a bovine large intestinal mucosal explant model

AU - Baehler, Angela A.

AU - Moxley, Rodney A

PY - 2002/6/18

Y1 - 2002/6/18

N2 - The effects of culture conditions on the extent of Escherichia coli O157:H7 attaching-effacing (A/E) adherence in an adult bovine large intestinal mucosal explant model were assessed by three different morphometric methods. Measurement of the percent of tissue sections with A/E adherence and the number of foci of A/E adherence mm-1 of surface epithelium was more sensitive than measurement of the percent of surface epithelium with A/E adherent bacteria for detection of treatment effects. Culture of bacterial inoculum in tryptic soy broth, incubation of explants in 5% CO2, and rocking of explants on a platform rocker at 18 cycles min-1 provided optimal conditions for A/E adherence. In future studies, the model may be used for preliminary testing of intervention strategies aimed at reduction of E. coli O157:H7 intestinal colonization of cattle.

AB - The effects of culture conditions on the extent of Escherichia coli O157:H7 attaching-effacing (A/E) adherence in an adult bovine large intestinal mucosal explant model were assessed by three different morphometric methods. Measurement of the percent of tissue sections with A/E adherence and the number of foci of A/E adherence mm-1 of surface epithelium was more sensitive than measurement of the percent of surface epithelium with A/E adherent bacteria for detection of treatment effects. Culture of bacterial inoculum in tryptic soy broth, incubation of explants in 5% CO2, and rocking of explants on a platform rocker at 18 cycles min-1 provided optimal conditions for A/E adherence. In future studies, the model may be used for preliminary testing of intervention strategies aimed at reduction of E. coli O157:H7 intestinal colonization of cattle.

KW - Attaching-effacing

KW - Cattle

KW - Escherichia coli O157:H7

KW - Explant

KW - Large intestine

KW - Morphometry

UR - http://www.scopus.com/inward/record.url?scp=0037129836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037129836&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(02)00718-8

DO - 10.1016/S0378-1097(02)00718-8

M3 - Article

VL - 212

SP - 107

EP - 110

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -