Effect of C/N ratios on nitrogen removal and microbial communities in the anaerobic baffled reactor (ABR) with an anammox-coupling-denitrification process

Chongjun Chen, Min Zhang, Xuliang Yu, Juan Mei, Ying Jiang, Yaoqi Wang, Tian C Zhang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Effects of different C/N (NO 2 - N) ratios on nitrogen removal and microbial community structure were investigated using an anaerobic baffled reactor (ABR). Results indicated that the C/N ratio exerted an important effect on nitrogen removal in the anammox-coupling-denitrification process associated with the ABR. When the C/N ratio was 1.29, the ABR could achieve the highest total nitrogen (TN) removal efficiency of 99.9%. Most of TN was removed in the 1st and 2nd compartment, accounting for about 81.0-97.6% of total TN removal. The nitrogen removal resulted from the interaction among anammox, heterotrophic denitrificans, and other microbes within the ABR. The contribution of anammox to nitrogen removal varied from 6.8% to 32.4%. High-throughput MiSeq sequencing analyses revealed that the C/N ratio was one of the most important factors regulating the microbial community structure, and the predominant phylum changed from Proteobacteria to Chloroflexi with the elevated C/N ratio. In addition, the Candidatus Brocadia was the major anammox bacterium, and its percentage varied from 1.0-2.9% at day 9 to 2.8-9.1% at day 46.

Original languageEnglish (US)
Pages (from-to)2338-2348
Number of pages11
JournalWater Science and Technology
Volume78
Issue number11
DOIs
StatePublished - Dec 28 2018

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Nitrogen removal
Denitrification
denitrification
microbial community
nitrogen
community structure
effect
removal
reactor
Bacteria
Throughput
Nitrogen
bacterium

Keywords

  • Anaerobic baffled reactor (ABR)
  • Anammox
  • C/N ratio
  • Denitrification
  • Microbial community structure
  • MiSeq sequencing analysis

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

Cite this

Effect of C/N ratios on nitrogen removal and microbial communities in the anaerobic baffled reactor (ABR) with an anammox-coupling-denitrification process. / Chen, Chongjun; Zhang, Min; Yu, Xuliang; Mei, Juan; Jiang, Ying; Wang, Yaoqi; Zhang, Tian C.

In: Water Science and Technology, Vol. 78, No. 11, 28.12.2018, p. 2338-2348.

Research output: Contribution to journalArticle

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abstract = "Effects of different C/N (NO 2 - N) ratios on nitrogen removal and microbial community structure were investigated using an anaerobic baffled reactor (ABR). Results indicated that the C/N ratio exerted an important effect on nitrogen removal in the anammox-coupling-denitrification process associated with the ABR. When the C/N ratio was 1.29, the ABR could achieve the highest total nitrogen (TN) removal efficiency of 99.9{\%}. Most of TN was removed in the 1st and 2nd compartment, accounting for about 81.0-97.6{\%} of total TN removal. The nitrogen removal resulted from the interaction among anammox, heterotrophic denitrificans, and other microbes within the ABR. The contribution of anammox to nitrogen removal varied from 6.8{\%} to 32.4{\%}. High-throughput MiSeq sequencing analyses revealed that the C/N ratio was one of the most important factors regulating the microbial community structure, and the predominant phylum changed from Proteobacteria to Chloroflexi with the elevated C/N ratio. In addition, the Candidatus Brocadia was the major anammox bacterium, and its percentage varied from 1.0-2.9{\%} at day 9 to 2.8-9.1{\%} at day 46.",
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