Effect of CD80 and CD86 on t cell cytokine production

Thomas M Petro, Swey Shen A Chen, Robert B. Panther

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Conjugation of the T cell receptor (TCR) with antigen/MHC proteins must be accompanied by conjugation of T cell counterreceptors (CD28 or CTLA-4) with costimulatory molecules CD80 or CD86 (B7-1 or B7-2) on antigen presenting cells (APC) to avert T cell anergy, and to provide essential signals for T cell activation and cytokine production. However, T cells and APC express changing patterns of counterreceptors and costimulatory molecules during the immune response. To determine the involvement of CD80 and CD86 in costimulation of T cell cytokine production, T cells were incubated with peritoneal exudate macrophages, which express CD80 and CD86, and stimulated in vitro for 48 or 72 hrs with anti-CD3 in the presence or absence of blocking antibody to CD80 or CD86. Alternatively, enriched anti-CD3 stimulated T cells were costimulated with antibody to CD28 and CTLA-4. Production of T cell IL-2, IL-4, and IL-5 was depressed in the presence of anti-CD86 but not anti-CD80. Production of IFN-γ was significantly blocked by either anti-CD80 and anti-CD86. Anti-CD28 was a potent costimulator of IFN-γ and IL-2 production, but a less potent costimulator of IL-4 and IL-5 production. The data suggest that T cell counterreceptors and APC costimulatory molecules act with varying efficacies at stimulating production of T cell cytokines.

Original languageEnglish (US)
Pages (from-to)965-976
Number of pages12
JournalImmunological Investigations
Volume24
Issue number6
DOIs
StatePublished - Jan 1 1995

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Cytokines
T-Lymphocytes
Antigen-Presenting Cells
Viral Tumor Antigens
Interleukin-5
Interleukin-4
Interleukin-2
CD86 Antigens
Blocking Antibodies
Peritoneal Macrophages
Exudates and Transudates
T-Cell Antigen Receptor
Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Effect of CD80 and CD86 on t cell cytokine production. / Petro, Thomas M; Chen, Swey Shen A; Panther, Robert B.

In: Immunological Investigations, Vol. 24, No. 6, 01.01.1995, p. 965-976.

Research output: Contribution to journalArticle

Petro, Thomas M ; Chen, Swey Shen A ; Panther, Robert B. / Effect of CD80 and CD86 on t cell cytokine production. In: Immunological Investigations. 1995 ; Vol. 24, No. 6. pp. 965-976.
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