Effect of caffeine on radiation-induced apoptosis in TK6 cells

Weining Zhen, A. T M Vaughan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Apoptosis has been measured in cells of the human TK6 lymphoblastoid cell line by recording the release of endonuclease-digested DNA from affected cells using flow cytometry. In asynchronously dividing cells, DNA degradation characteristic of apoptosis was first seen 12 h after irradiation as a defined DNA fluorescent peak of sub-G1-phase content, reaching a maximum of 30-50% of the population by 24-72 h. Treating cells with 2 mM caffeine either before or up to 3 h after irradiation eliminated the degradation of DNA entirely. In addition, the percentage of cells in which apoptosis could be detected microscopically decreased from 62.4 ± 0.95% to 16.7± 1.5% 72 h after caffeine treatment. Delaying caffeine treatment for 12 h after irradiation reduced DNA degradation by approximately 50% compared to cells receiving radiation alone. DNA degradation induced by serum deprivation was unaffected by caffeine treatment. These data support the contention that irradiation of TK6 cells produces a long-lived cellular signal which triggers apoptosis. Apoptosis produced by serum deprivation does not operate through the same pathway.

Original languageEnglish (US)
Pages (from-to)170-175
Number of pages6
JournalRadiation Research
Volume141
Issue number2
DOIs
StatePublished - Apr 5 1995

Fingerprint

caffeine
apoptosis
Caffeine
Radiation
Apoptosis
deoxyribonucleic acid
radiation
cells
DNA
irradiation
deprivation
degradation
serums
cytometry
Deoxyribonuclease I
G1 Phase
Serum
cultured cells
interphase
flow cytometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Effect of caffeine on radiation-induced apoptosis in TK6 cells. / Zhen, Weining; Vaughan, A. T M.

In: Radiation Research, Vol. 141, No. 2, 05.04.1995, p. 170-175.

Research output: Contribution to journalArticle

Zhen, Weining ; Vaughan, A. T M. / Effect of caffeine on radiation-induced apoptosis in TK6 cells. In: Radiation Research. 1995 ; Vol. 141, No. 2. pp. 170-175.
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