Effect of avanafil on rat and human corpus cavernosum

Serap Gur, S. C. Sikka, E. A. Pankey, G. F. Lasker, S. Chandra, P. J. Kadowitz, W. J.G. Hellstrom

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We compared the activity of a new phosphodiesterase-5 inhibitor (PDE5i) avanafil with sildenafil and tadalafil in human and rat corpus cavernosum (CC) tissues. The effect of avanafil with several inhibitors and electrical field stimulation (EFS) was evaluated on CC after pre-contraction with phenylephrine. With the PDE5i, sildenafil and tadalafil, concentration-response curves were obtained and cyclic guanosine monophosphate (cGMP) levels were measured in tissues. Avanafil induced relaxation with maximum response of 74 ± 5% in human CC. This response was attenuated by NOS inhibitor and soluble guanylate cyclase (sGC) inhibitor. Avanafil potentiated relaxation responses to acetylcholine and EFS in human CC and enhanced SNP-induced relaxation and showed 3-fold increase in cGMP levels. When compared with sildenafil, avanafil and tadalafil were effective at lower concentrations in human CC. In addition, Sprague-Dawley rats underwent in vivo intracavernosal pressure (ICP) and mean arterial pressure (MAP) measurements. Avanafil increased ICP/MAP that was enhanced by SNP and cavernous nerve (CN) stimulation in rat CC tissues. Also avanafil showed maximum relaxation response of 83 ± 7% in rat CC with 3-fold increase in cGMP concentration. Taken together, these results of our in vivo and in vitro studies in human and rat suggest that avanafil promotes the CC relaxation and penile erection via NO-cGMP pathway.

Original languageEnglish (US)
Pages (from-to)897-903
Number of pages7
JournalAndrologia
Volume47
Issue number8
DOIs
StatePublished - Oct 1 2015

Fingerprint

Cyclic GMP
Phosphodiesterase 5 Inhibitors
Electric Stimulation
Single Nucleotide Polymorphism
Arterial Pressure
Penile Erection
Pressure
avanafil
Phenylephrine
Acetylcholine
Sprague Dawley Rats
Tadalafil
Sildenafil Citrate

Keywords

  • Avanafil
  • Human and rat corpus cavernosum
  • Nitric oxide
  • Relaxation
  • cGMP-electrical field stimulation

ASJC Scopus subject areas

  • Endocrinology
  • Urology

Cite this

Gur, S., Sikka, S. C., Pankey, E. A., Lasker, G. F., Chandra, S., Kadowitz, P. J., & Hellstrom, W. J. G. (2015). Effect of avanafil on rat and human corpus cavernosum. Andrologia, 47(8), 897-903. https://doi.org/10.1111/and.12344

Effect of avanafil on rat and human corpus cavernosum. / Gur, Serap; Sikka, S. C.; Pankey, E. A.; Lasker, G. F.; Chandra, S.; Kadowitz, P. J.; Hellstrom, W. J.G.

In: Andrologia, Vol. 47, No. 8, 01.10.2015, p. 897-903.

Research output: Contribution to journalArticle

Gur, S, Sikka, SC, Pankey, EA, Lasker, GF, Chandra, S, Kadowitz, PJ & Hellstrom, WJG 2015, 'Effect of avanafil on rat and human corpus cavernosum', Andrologia, vol. 47, no. 8, pp. 897-903. https://doi.org/10.1111/and.12344
Gur S, Sikka SC, Pankey EA, Lasker GF, Chandra S, Kadowitz PJ et al. Effect of avanafil on rat and human corpus cavernosum. Andrologia. 2015 Oct 1;47(8):897-903. https://doi.org/10.1111/and.12344
Gur, Serap ; Sikka, S. C. ; Pankey, E. A. ; Lasker, G. F. ; Chandra, S. ; Kadowitz, P. J. ; Hellstrom, W. J.G. / Effect of avanafil on rat and human corpus cavernosum. In: Andrologia. 2015 ; Vol. 47, No. 8. pp. 897-903.
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AU - Kadowitz, P. J.

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