Effect of adhesive agent and fissure morphology on the in vitro microleakage and penetrability of pit and fissure sealants

Derek Marks, Barry M. Owens, William W. Johnson

Research output: Contribution to journalArticle

15 Scopus citations


Objective: To evaluate the effect of adhesive agents and fissure morphology on the microleakage and penetrability of pit and fissure sealants. Method and Materials: Sealants used in this study included Aegis (Bosworth), conseal f (Southern Dental Industries), and Admira Seal (Voco). Adhesive agents included Optibond Solo Plus (sds/Kerr) and Clearfil S3Bond (Kuraray). Ninety extracted permanent molars were randomly assigned to 9 groups combining sealant and adhesive agent materials. A sealant and control group (phosphoric acid etch only) was also included. Dye penetration (microleakage), penetrability, and fissure morphology assessment was performed for the treatment groups through microscopic evaluation. Results: Significant (P = .003) differences in microleakage were exhibited, with the Aegis + control and Aegis + Optibond Solo Plus groups displaying less leakage, while significant (P = .03) differences were also noted between groups regarding penetrability. Fissure morphology was not a significant (P = .82) factor affecting microleakage; however, fissure type did significantly (P < .001) impact penetrability. No correlation was found between the extent of microleakage and penetrability. Conclusion: Application of sealants using phosphoric acid as a conditioning agent revealed superior results, while the use of adhesives was found to be unnecessary.

Original languageEnglish (US)
Pages (from-to)763-772
Number of pages10
JournalQuintessence international
Issue number9
Publication statusPublished - Jan 1 2009



  • Adhesive
  • Fissure morphology
  • Microleakage
  • Pit and fissure sealant

ASJC Scopus subject areas

  • Dentistry(all)

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